Novel oligosaccharides isolated from Fusarium oxysporum L. rapidly induced PAL activity in Rubus cells

• Autorzy: Nita-Lazar M. , Heyraud A. , Gey C. , Braccini I. , Lienart Y.
• Języki publikacji: EN

Abstrakty

EN

Activation of the phenolic pathway is known to be part of a defense response against cell wall-derived elicitors from pathogens. Many examples of a defense re­sponse by increasing the synthesis of phenolic compound against the elicitor were demonstrated in the past, but the elicitor structure has so far been poorly character­ized. Our results indicate that a disaccharide fraction containing the following structure: a-D-mannopyranosyl (1=>2)a/ß-D-glucopyranosyl and a-D-mannopyranosyl (1=>x) ino­sitol, isolated from Fusarium oxysporum L., promotes rapid and transient phenylalanine ammonia lyase activity in Rubus fructicosus cells at nanomolar con­centration. The disaccharides were isolated by size-exclusion chromatography di­rectly from extracts obtained by alkaline treatment of F. oxysporum mycelium. Their structure was determined by 500-MHz-1H-NMR spectroscopy combined with methylation analysis and fast atom bombardment mass spectrometry.

Słowa kluczowe

EN

phenylalanine ammonia lyase; Rubus fructicosus; Fusarium oxysporum; fungi; oligosaccharide elicitor; Fusarium

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2004
• Tom: 51
• Numer: 3
• Opis fizyczny: p.625-634,fig.,ref.

Twórcy

autor

Nita-Lazar M.

• Centre de Recherches sur les Macromolecules Vegetales, Grenoble, France

autor

Heyraud A.

autor

Gey C.

autor

Braccini I.

autor

Lienart Y.

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