Collagen type II modification by hypochlorite

• Autorzy: Olszowski S. , Mak P. , Olszowska E. , Marcinkiewicz J.
• Języki publikacji: EN

Abstrakty

EN

Oxidation of proteins is a common phenomenon in the inflammatory process medi­ated by highly reactive agents such as hypochlorite (HOCl/OCl-) produced by acti­vated neutrophils. For instance, in rheumatoid arthritis hypochlorite plays an impor­tant role in joint destruction. One of the major targets for HOCl/OCl- is collagen type II (CII) — the primary cartilage protein. In our study, HOCl/OCl- mediated collagen II modifications were tested using various methods: circular dichroism (CD), HPLC, ELISA, dynamic light scattering (DLS), fluorimetry and spectrophotometry. It was shown that hypochlorite action causes deamination with consecutive carbonyl group formation and transformation of tyrosine residues to dichlorotyrosine. Moreover, it was shown that ammonium chloramine (NH^Cl) formed in the reaction mixture reacts with CII. However, in this case the yield of carbonyl groups and dichlorotyrosine is lower than that observed for HOCl/OCl- by 50%. CD data revealed that collagen II ex­ists as a random coil in the samples and that chlorination is followed by CII fragmenta­tion. In the range of low HOCl/OCl- concentrations (up to 1 mM) 10-90 kDa peptides are predominant whereas massive production of shorter peptides was observed for high (5 mM) hypochlorite concentration. DLS measurements showed that chlorina- tion with HOCl/OCl- decreases the radius of collagen II aggregates from 30 to 6.8 nm. Taking into account the fact that chlorinated collagen is partially degraded, the DLS results suggest that smaller micelles are formed of the 10-90 kDa peptide fraction. Moreover, collagen chlorination results in epitope modification which affects CII rec­ognition by anti-CII antibodies. Finally, since in the synovial fluid the plausible hypochlorite concentration is smaller than that used in the model the change of size of molecular aggregates seems to be the best marker of hypochlorite-mediated collagen oxidation.

Słowa kluczowe

EN

hypochlorite; highly reactive agent; oxidation; taurine chloramine; neutrophil; inflammatory process; protein; oxidative modification; collagen; rheumatoid arthritis; modification

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2003
• Tom: 50
• Numer: 2
• Opis fizyczny: p.471-479,fig.

Twórcy

autor

Olszowski S.

• Collegium Medicum, Jagiellonian University, Czysta 18, 31-121 Krakow, Poland

autor

Mak P.

autor

Olszowska E.

autor

Marcinkiewicz J.

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