Differential response of antioxidant enzymes to cadmium stress in tolerant and sensitive cell line of cucumber [Cucumis sativus L.]

• Autorzy: Gzyl J. , Rymer K. , Gwozdz E.A.
• Języki publikacji: EN

Abstrakty

EN

Previously, a stable cell suspension of cucumber tolerant to 100 µM CdCl2 was obtained (Gzyl & Gwóźdź, 2005, Plant Cell Tissue Organ Cult 80: 59-67). In this study, the relationship between the activity of antioxidant enzymes and cadmium tolerance of cucumber cells was analyzed. A cadmium-sensitive and the cadmium-tolerant cell lines were exposed to 100 µM and 200 µM CdCl2 and the activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APOX) and guaiacol peroxidase (POX) were determined. In the sensitive cell line, a decrease of total activity of SOD and POX was observed, whereas the activity of CAT and APOX significantly increased in metal-supplemented medium. By contrast, in the tolerant cells, the total activity of antioxidant enzymes decreased (SOD, CAT) or was maintained at approximately the same level (APOX, POX). Moreover, a different pattern of isoenzyme activity was observed in the tolerant and sensitive cells. These results suggest that an enhanced activity of antioxidant enzymes is not directly involved in the increased tolerance to cadmium of the selected cucumber cell line.

Słowa kluczowe

EN

ascorbate peroxidase; cadmium; catalase; antioxidative enzyme; cadmium stress; cadmium-tolerant cell line; tolerance; guaiacol peroxidase; superoxide dismutase; cucumber; Cucumis sativus; sensitive cell line; cell suspension

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2009
• Tom: 56
• Numer: 4
• Opis fizyczny: p.723-727,fig.,ref.

Twórcy

autor

Gzyl J.

• Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland

autor

Rymer K.

autor

Gwozdz E.A.

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