Okreslenie sekwencji kodujacej region VP1 wirusa pryszczycy typu O

• Autorzy: Paprocka G , Plucienniczak G. , Plucienniczak A. , Kesy A. , Niedbalski W. , Fitzner A.
• Języki publikacji: PL

Abstrakty

EN

A Polish strain of foot-and-mouth disease virus type О from the laboratory virus collection was used. The virus was propagated in BHK-21 monolayer cells. Total RNA was extracted by the Chomczyński and Sacchi method. A synthesis of cDNA was performed with AMV-reverse transcriptase. The first and second cDNA strands were used as a template for the amplification by PCR of the 672 bp of the VP1 coding sequence. The amplified fragment of cDNA was cloned in the phagemid pBS (+). The first DNA strand expressed in the phage M13K07 was sequenced according to the Sanger method. The nucleotide sequences were compared to the earlier data published. Genetic similarities to the Brasilian strain of FMDV O1 Campos 58 were found.

Słowa kluczowe

PL

wirus pryszczycy typu O; serotypy; region VP1; wirusy; lancuchowa reakcja polimerazy; sekwencja nukleotydow; DNA komplementarny; zmiennosc antygenowa

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 1996
• Tom: 52
• Numer: 02
• Opis fizyczny: s.111-113,fot.,bibliogr.

Twórcy

autor

Paprocka G

• Zaklad Pryszczycy Panstwowego Instytutu Weterynaryjnego, ul. Wodna 7, 98-220 Zdunska Wola

autor

Plucienniczak G.

autor

Plucienniczak A.

autor

Kesy A.

autor

Niedbalski W.

autor

Fitzner A.

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