Characterisation of Staphylococcus aureus strains isolated from cows' milk

• Autorzy: Bystron J , Bania J. , Lis E. , Molenda J. , Bednarski M.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to characterise the Staphylococcus aureus strains isolated from unprocessed cow's milk for staphylococcal superantigenic toxin genes (sea-selu and tst), to examine the presence of mecA gene and to determine the type of staphylococcal protein A (spa) gene. The type of the gene was determined for all 68 Staphylococcus aureus strains, based on the polymorphism of a number of repeated motifs found in the region X of spa. The genes forming the egc 1 cluster, i.e. seg, sei, selm, seln, and selo were found to be the most prevalent (29.4%). Three isolates (4.41%) carried seh and one (1.47%) the seb/selk genes. No tst and mecA genes were found in the examined S. aureus. Spa types containing three and six to 14 repeats were found in the studied S. aureus population. Strains harbouring eight, ten, and twelve repeats were most frequent (33.82%, 29.41%, and 13.23%, respectively).

Słowa kluczowe

EN

isolation; enterotoxin gene; determination; mecA gene; cow milk; milk; tst gene; Staphylococcus aureus

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2009
• Tom: 53
• Numer: 1
• Opis fizyczny: p.59-63,fig.,ref.

Twórcy

autor

Bystron J

• Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland

autor

Bania J.

autor

Lis E.

autor

Molenda J.

autor

Bednarski M.

Bibliografia

• 1. Akineden Ö., Hassan A.A., Schneider E., Usleber E.: Enterotoxigenic properties of Staphylococcus aureus isolated from goat's milk cheese. Int J Food Microbiol 2008, 124, 211-216.
• 2. Anon.: Infectious diseases and poisonings in Poland in 2005-2006. Bull Nat Inst Hygiene, Warsaw, 2008.
• 3. Asperger H., Zangerl P.: Staphylococcus aureus. In: Encyclopedia of Dairy Sciences, vol. 4, edited by Roginski H., Fuquay J.W., Fox P.F., Academic Press and Elsevier Science, 2003, pp. 2563-2569.
• 4. Balaban N., Rasooly A.: Staphylococcal enterotoxins. Int J Food Microbiol. 2000, 61, 1-10.
• 5. Bania J., Dąbrowska A., Bystroń J., Korzekwa K., Chrzanowska J., Molenda J.: Distribution of newly described enterotoxin-like genes in Staphylococcus aureus from food. Int J Food Microbiol 2006, 108, 36-41.
• 6. Bania J., Dąbrowska A., Korzekwa K., Żarczyńska A., Bystroń J., Chrzanowska J., Molenda J.: The profiles of enterotoxin genes in Staphylococcus aureus from nasal carriers. Lett Appl Microbiol 2006, 42, 315-320.
• 7. Boye K., Bartels M.D., Andersen I.S., Møller J.A., Westh H.: A new multiplex PCR for easy screening of methicilin-resistant Staphylococcus aureus SCCmec types I-V. Clin Microbiol Infect 2007, 13, 725-727.
• 8. Colburn K.K., Wong L.G., Ashman R.F., Wistar R.Jr, Weisbart R.H.: Effect of immunoglobulin G on the responsiveness of human neutrophils to soluble staphylococcal protein A-induced neutrophil migration inhibition factor from T-lymphocytes. Infect Immun 1980, 30, 674-677.
• 9. Dalla Pozza M.C., Ricci A., Vicenzoni G.: Protein A gene polymorphism analysis in Staphylococcus aureus strains isolated from bovine subclinical mastitis. J Dairy Res 1999, 30, 674-677.
• 10. Dinges M., Orwin P.M., Schlievert P.M.: Exotoxins of Staphylococcus aureus. Clin Microbiol Rev 2000, 13, 16- 34.
• 11. Frénay H.M., Theelen J.P., Schouls L.M., Vandenbroucke-Grauls C.M., Verhoef J., van Leeuwen W.J., Moi F.R.: Discrimination of epidemic and nonepidemic methicilin-resistant Staphylococcus aureus strains on the basis of protein A gene polymorphism. J Clin Microbiol 1994, 32, 846-847.
• 12. Haveri M., Roslöf A., Rantala L., Pyörälä S.: Virulence genes of bovine Staphylococcus aureus from persistent and nonpersistent intramammary infections with different clinical characteristics. J Appl Microbiol 2007, 103, 993-1000.
• 13. Jablonsky L.M., Bohach G.: Staphylococcus aureus. In: Food Microbiology Fundamentals and Frontiers. Edited by Doyle M.P., Beuchat L.R., Montevile T.J., ASM Press, Washington DC 1997, pp. 353-357.
• 14. Jarraud S., Peyrat M.A., Lim A., Tristan A., Bes M., Mougel C., Etienne J., Vandenesch F., Bonneviille M., Lina G.: egc, a highly prevalent operon of enterotoxin gene, forms a putative nursery of superantigenes in Staphylococcus aureus. J Immunol 2001, 166, 669-677.
• 15. Jorgensen HJ., Mork T., Hogasen H.R., Rovik L.M.: Enterotoxigenic Staphylococcus aureus in bulk milk in Norway. J Appl Microbiol 2005, 99, 158-166.
• 16. Kuźma K., Malinowski E., Lassa H., Kłossowska A.: Analysis of protein A gene polymorphism in Staphylococcus aureus isolates from bovine mastitis. Bull Vet Inst Pulawy 2005, 49, 41-44.
• 17. Kwon N.H., Park K.T., Moon J.S., Jung W.K., Kim S.H., Kim J.M., Hong S.K., Koo H.C., Joo Y.S., Park Y.H.: Staphylococcal cassette chromosome mec (SCCmec) characterization and molecular analysis for methicilin-resistant Staphylococcus aureus and novel SCCmec subtype IVg isolated from bovine milk in Korea. J Antimicrob Chemother 2005, 56, 624-632.
• 18. Lee J.H.: Methicilin (oxacillin)-resistant Staphylococcus aureus strains isolated from major food animals and their potential transmission to humans. Appl Environ Microbiol 2003, 69, 6489-6494.
• 19. Letertre C., Perelle S., Dilasser F., Fach P.: Identification of a new putative enterotoxin SEU encoded by the egc cluster of Staphylococcus aureus. J Appl Microbiol 2003, 95, 38-43.
• 20. Lowy F.D.: Staphylococcus aureus infections. N Engl J Med 1998, 339, 520-532.
• 21. Malinowski E., Lassa H., Kłossowska A., Markiewicz H., Kaczmarowski M., Smólski S.: Relationship between mastitis agents and somatic cell count in foremilk samples. Bull Vet Inst Pulawy 2006, 50, 349-352.
• 22. Martin M.C., Gonzales-Hevia M.A., Mendoza M.C.: Usefulness of a two-step PCR procedure for detection and identification of enterotoxigenic staphylococci of bacterial isolates and food samples. Food Microbiol 2003, 20, 605-610.
• 23. Molenda J., Bania J., Bystroń J., Twardoń J.: Importance of newly detected staphylococcal enterotoxins for food safety. Pol J Vet Sci 2008, 11, 79-82.
• 24. Montesinos I., Salido E., Delgado T., Cuervo M., Sierra A.: Epidemiologic genotyping of methicilin-resistant Staphylococcus aureus by pulsed-field gel electrophoresis at a University Hospital and comparison with antibiotyping and protein A and coagulase gene polymorphisms. J Clin Microbiol 2002, 40, 2119-2125.
• 25. Musher D.M., Verbrugh H.A., Verhoef J.: Suppression of phagocytosis and Chemotaxis by cell wall components of Staphylococcus aureus. J Immunol 1981, 127, 84-88.
• 26. Normanno G., Corrente M., La Salandra G., Dambrosio N.C., Quaglia N.C., Parisi A., Greco G., Bellacicco A.L., Virgilio S., Celano G.V.: Methicilin-resistant Staphylococcus aureus (MRSA) in foods of animal origin product in Italy. Int J Food Microbiol 2007, 117, 219-222.
• 27. Normanno G., La Salandra G., Dambrosio N.C., Quaglia N.C., Corrente M., Parisi A., Santagada G., Firinu A., Crisetti E., Celano G.V.: Occurrence, characterization and antimicrobial resistance of enterotoxigenic Staphylococcus aureus isolated from meat and dairy products. Int J Food Microbiol 2007, 115, 290-296.
• 28. Omoe K., Hu D.L., Takahashi-Omoe H., Natane A., Shinagawa K.: Identification and characterization of a new staphylococcal enterotoxin-related putative toxin encoded by two kinds of plasmids. Infect Immun 2003, 71, 6088-6094.
• 29. Orwin P.M., Leung D.Y.M., Donahue H.L., Novick R.P., Schliviert P.M.: Biochemical and biological properties of staphylococcal enterotoxin K. Infect Immun 2001, 69, 360-366.
• 30. Peles F., Wagner M., Varga L., Hein I., Rieck P., Gutser K., Keresztúri P., Kardos G., Turcsányi I., Béri B., Szabó A.: Characterization of Staphylococcus aureus strains isolated from bovine milk in Hungary. Int J Food Microbiol 2007, 118, 186-193.
• 31. Rall V.L.M., Vieira F.P., Rall R., Vieitis R.L., Fernandes Jr A., Candeias J.M.G., Cardoso K.F.G., Araújo Jr J.P.: PCR detection of staphylococcal enterotoxin genes in Staphylococcus aureus strains isolated from raw and pasteurized milk. Vet Microbiol 2008, 132, 408-413.
• 32. Sharma N.K., Ress C.E., Dod C.E.: Development of a single-reaction multiplex PCR toxin typing assay for Staphylococcus aureus strains. Appl Environ Microbiol 2000, 66, 1347-1353.
• 33. Scherrer D., Corti S., Muehlherr J.E., Zweifel C., Stephan R.: Phenotypic and genotypie characteristics of Staphylococcus aureus isolates from raw bulk-tank milk samples of goats and sheep. Vet Microbiol 2004, 101, 101-107.
• 34. Vandenesch F., Naimi T., Enright M.C., Lina G., Nimmo G.R., Heffernan H., Liassine N., Bes M., Greenland T., Reverdy M.E., Etienne J.: Community-acquired methicilin-resistant Staphylococcus aureus carrying Panton-Valentine leukocidine genes: worldwide emergence. Emerg Infect Dis 2003, 9, 978-984.
• 35. Van Loo I., Diederen B., Savelkoul P., Woudenberg J., Roosendaal R., Van Belkum A., Den Toom N., Verhulst C., Van Keulen P., Kluytmans J.: Methicillin-resistant Staphylococcus aureus in meat products, the Netherlands. Emerg Infect Dis 2007, 13, 1753-1755.
• 36. Yarwood J.M., McCormick J.K., Paustian M.L., Orwin P.M., Kapur V., Schlievert P.M.: Characterization and expression analysis of Staphylococcus aureus pathogenicity island 3. J Biol Chem 2002, 277, 13138-13147.