High molecular mass dextran sulfate increases expression of HIV-1 coreceptor CCR-5 in macrophage-monocytes in culture

• Autorzy: Jagodzinski P. P. , Trzeciak W.H.
• Języki publikacji: EN

Abstrakty

EN

It has been reported that high molecular mass dextran sulfate (HMDS) enhances the infection of monocyte-macrophages by HIV-1. We observed that in monocyte-macrophages maintained in the presence of HMDS the expression of HIV-1 coreceptor CCR-5 was increased approximately 5-fold at the transcriptional level. We postulate that the increased expression of CCR-5 might be responsible for HMDS-enhanced infectivity of monocyte-macrophages by HIV-1.

Słowa kluczowe

EN

high molecular dextran sulphate; macrophage; HIV virus; low molecular mass dextran sulphate; AIDS; 1,3-beta-glucan sulphate; immunodeficiency

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1998
• Tom: 45
• Numer: 1
• Opis fizyczny: p.241-244,fig.

Twórcy

autor

Jagodzinski P. P.

• University of Medical Sciences, H.Swiecickiego 6, 60-781 Poznan, Poland

autor

Trzeciak W.H.

Bibliografia

• 1. Sattentau, Q.J. & Weiss, R.A. (1988) TheCD4 antigen: Physiological ligand and HIV recep­tor. Cell 52, 631-633.
• 2. Maddon, P.J. (1986) The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brain. Cell 47, 333-348.
• 3. Koito, A., Stamatatos, L. & Cheng-Mayer, C. (1995) Small amino acid sequence changes within the V2 domain can affect the function of a T-cell line-tropic human immunodefi­ciency virus type 1 envelope gpl20. Virology 206, 878-884.
• 4. Ashorn, P.A., Berger, E.A. & Moss, B.J. (1990) Human immunodeficiency virus enve­lope glycoprotein/CD4-mediated fusion of nonprimate cells with human cells. J. Virol 64, 2149-2156.
• 5. Feng, Y., Broder, C.C., Kennedy, P. & Berger, E.A. (1996) HIV-1 entry cofactor: Functional cDNA cloning of a seven-transmembrane G protein-coupled receptor. Science 272, 872- 876.
• 6. Alkhatib, G., Combadiere, C., Broder, C.C., Feng, Y., Kennedy, P.E.. Murphy, P.M. & Berger, E.A. (1996) CCCKR5: a RANTES, MIP-lö, MIP-1/? receptor as a fusion cofactor for macrophage-tropic HIV-1. Science 272, 1955-1958.
• 7. Bleul, C.C., Farzan, M., Choe, H., Parolin, C., Lewis, C.I., Sodrowski, J. & Springer, T.A. (1996) The lymphocyte chemoattractant SDF- 1 is a ligand for LESTR/fusin and blocks H1V- 1 entry. Nature (London) 382. 829-832.
• 8. Mcylan, P.R.A., Kornbluth. R.S., Zbinden, I. & Richman, D.D. (1994) Influence of host cell type and V3 loop of the surface glycoprotein on susceptibility of human immunodeficiency virus type 1 to polyanion compounds. Antimi- crob. Agents Chemother. 35, 2910-2916.
• 9. Toohey, K., Wehrly, K., Nishio, J., Ferry- man, S. &Chesebro, B. (L995) Human immu­nodeficiency virus envelope VI and V2 regions influence replication efficiency in macro­phages by affecting virus spread. Virology 213, 70-79.
• 10. Chomczynski, P. & Sacchi, N. (1985) Single- step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extrac­tion. Anal. Biochem. 162, 156-159.
• 11. Sugawara, I. & Ishizaka, S. (1984) Polysaccha­rides with sulfate groups are human T cell mi­togens and murine polyclonal B cell activators (PBAs). II. Cellulose sulfate and dextran sul­fate with two different lower molecular weights. Microbiol Immunol. 28, 831-839.