Distribution of the polymorphic variants of genes RYR1, LEP, GH, MYOG, MYF5, and GDF8 in wild boars from North-East of Poland

• Autorzy: Kuryl J , Zurkowski M. , Urbanski P. , Wyszynska-Koko J.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to characterize the polymorphism of genes RYR1, LEP, GH, MYOG, MYF5 and GDF8 in wild boar population inhabiting North-East of Poland. The above genes are considered candidate genes for carcass quality traits in domestic pigs. Ninety animals were tested. Genes RYR1, LEP and MYF5 appeared to be monomorphic, whereas genes MYOG, GH and GDF8 showed polymorphism similar to that observed in European pig breeds. However, no PCR product was obtained for several animals when MYOG and GDF8 gene fragments were amplified, suggesting the difference between wild boars tested and domestic pig in sequence of the gene regions where primers annealed. Moreover,this suggests a possibility of identification of new variants of these genes in wild boar.

PL

Celem badań była charakterystyka polimorfizmu wybranych genów (RYR1, LEP, GH, MYOG, MYF5,GDF8) u dzików zamieszkujących północno-wschodni rejon Polski. Przebadano 90 zwierząt. Wybrane geny określane są mianem genów kandydujących (candidate genes) dla cech jakości tuszy świń ze względu na ich potencjalne oddziaływanie na te cechy. Trzy z nich (RYR1, LEP i MYF5) okazały się monomorficzne, podczas gdy pozostałe (MYOG, GH i GDF8) wykazały polimorfizm podobny do obserwowanego w europejskich rasach świń. Dla niektórych jednak zwierząt nie uzyskano produktu PCR w wyniku reakcji amplifikacji (powielania) fragmentów genów MYOG i GDF8, co sugeruje możliwość obecności mutacji w rejonach przyłączania starterów, które uniemożliwiły prawidłowy przebieg tego etapu reakcji PCR i w konsekwencji dalszych jej etapów. Można wnioskować zatem o możliwości identyfikacji u dzika nowych wariantów tych dwóch genów.

Słowa kluczowe

EN

pig; MYF5 gene; GDF8 gene; animal genetics; gene polymorphism; wild boar; GH gene; LEP gene; boar; MYOG gene; animal breeding; RYR1 gene

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2004
• Tom: 22
• Numer: 3
• Opis fizyczny: p.271-278,ref.

Twórcy

autor

Kuryl J

• Polish Academy of Sciences, Jastrzebiec, 05-552 Wolka Kosowska, Poland

autor

Zurkowski M.

autor

Urbanski P.

autor

Wyszynska-Koko J.

Bibliografia

• 1. ANDERSSON-EKLUND L., MARKLUND L., LUNDSTRÖM K., HALEY C.S., ANDERSSON K., HANSSON I., MOLLER M., ANDERSSON L., 1998 – Mapping quantitative trait loci for carcass and meat quality traits in a Wild Boar × Large White intercross. Journal of Animal Science 76, 694-700.
• 2. BUITKAMP J., ANTES R., WAGNER V., 1999 – DNA profilling in veterinary medicine. In: DNA profilling and DNA fingerprinting (Jörg T. Eppeln and Thomas Lubjuhn, Eds.), Birkhäuser Varlag, Basel-Boston-Berlin, pp.53-70.
• 3. CEPICA S., REINER G., BARTENSCHLAGER H., MOSER G., GELDERMANN H., 2003 – Linkage and QTL mapping for Sus scrofa chromosome X. Journal of Animal Breeding and Genetics 120 (Suppl. 1), 144-151.
• 4. CIEŚLAK D., KAPELAŃSKI W., BLICHARSKI T., PIERZCHAŁA M., 2000 – Restriction fragment length polymorphisms in myogenin and myf3 genes and their influence on lean meat content in pigs. Journal of Animal Breeding and Genetics 117, 43-55.
• 5. CIEŚLAK D., KURYŁ J., KAPELAŃSKI W., PIERZCHAŁA M., GRAJEWSKA S., BOCIAN M., 2002 – A relationship between genotypes at MYOG, MYF3 and MYF5 loci and carcass meat and fat deposition traits in pigs. Animal Science Papers and Reports 20, 77-92.
• 6. DRAGOS-WENDRICH M., STRATIL A., HOJNY J., MOSER G., BARTENSCHLAGER H.,REINER G., GELDERMANN H., 2003 – Linkage and QTL mapping for Sus scrofa chromosome 18.Journal of Animal Breeding and Genetics 120 (Suppl. 1), 138-143.
• 7. EPSTEIN H., 1984 – Pig. In: evolution of Domesticated Animals (I.L. Mason, Ed.) Longman, London,pp. 145-162.
• 8. ERNST M., KUCIEL J., URBAN T., 2003 - Analysis of genetic variation of eight candidate genes in two wild boar subspecies. Czech Journal of Animal Sciences 48, 533-539.
• 9. GELDERMANN H., MÜLLER E., MOSER G., REINER G., BARTENSCHLAGER H., CEPICA S., STRATIL A., KURYL J., MORAN C., DAVOLI R., BRUNSCH C., 2003 – Genome-wide linkage and QTL mapping in porcine F2 families generated from Pietrain, Meishan and Wild Boar crosses. Journal of Animal Breeding and Genetics 120, 363-393.
• 10. GIUFFRA E., KIJAS J.M.H., AMARGER V., CARLBORG Ő., JEON J.-T., ANDERSSON L., 2000 – The origin of the domestic pig: independent domestication and subsequent introgression. Genetics 154, 1785-1791.
• 11. GROBET L., PONCELET D., ROYO L.J., BROUWERS B., PIROTTIN D., MICHAUX C.,MENISSIER F., ZANOTTI M., DUNNER S., GEORGES M., 1998 – Molecular definition of an allelic series of mutations disrupting the myostatin function and causing double-muscling in cattle.Mammalian Genome 9, 210-213.
• 12. HARTL G.B., MARKOV G., RUBIN A., FINDO S., LANG G., WILLING R., 1993 – Allozyme diversity within and among populations of three ungulate species (Cervus elaphus, Capreolus capreolus, Sus scrofa) of Southeastern and Central Europe. Zeitschrift für Säugetierkunde 58, 352-361.
• 13. HEERE W., ROHRS M., 1977 – Zoological considerations on the origins of farming and domestication. In: Origins of Agriculture (Cinthia A. Reed, Ed.), Mouton, The Hague, pp. 245-279.
• 14. HUANG Y.-F., SHI X.-W., ZHANG Y.-P., 1999 – Mitochondrial genetic variation in Chinese pigs and wild boars. Biochemical Genetics 37, 335-343.
• 15. KAMIŃSKI S., RUŚĆ A., WOJTASIK K., 2001 – Jednoczesna identyfikacja genotypów RYR1 i ESR metodą multiplex PCR-RFLP u świń rasy wbp i pbz. (Simultaneous identification of RYR1 and ESR genotypes by multiplex PCR-RFLP in Polish Large White and Polish Landrace pigs). XIV Conference of the Polish Genetic Society, Poznań, 11-13 June 2001), Book of Abstracts, p. 54.
• 16. KIRKPATRICK B.W., 1992 – HaeII and MspI polymorphisms are detected in the second intron of the porcine growth hormone gene. Animal Genetics 23, 180-181.
• 17. KURYŁ J., 2000 - Geny cech ilościowych zwierząt gospodarskich – aktualny stan badań (The current state of research on the quantitative traits loci in farm animals – a review). Prace i Materiały Zootechniczne 56, 7-50.
• 18. KURYŁ J., KAPELAŃSKI W., PIERZCHAŁA M., BOCIAN M., GRAJEWSKA S., 2003 – A relationship between genotypes at the GH and LEP loci and carcass meat and fat deposition in pigs. Animal Science Papers and Reports 21, 15-26.
• 19. KURYŁ J., ŻURKOWSKI M., 1988 – Genetic markers in blood plasma of wild pigs identified by horizontal and 2-D polyacrylamide electrophoresis. XXIst International Conference on Animal Blood Groups and Biochemical Polymorphisms, Turin (Italy), Abstracts p. 59.
• 20. LEE S.S., CHEN Y., MORAN C., STRATIL A., REINER G., BARTENSCHLAGER H., MOSER G., GELDERMANN H., 2003 – Linkage and QTL mapping for Sus scrofa chromosome 5. Journal of Animal Breeding and Genetics 120 (Suppl. 1), 38-44.
• 21. MARKLUND L., 1997 – Genome Analysis of quantitative trait loci in the pig. Ph.D. Thesis, Swedish University of Agricultural Sciences, Uppsala, Sweden.
• 22. MÜLLER E., MOSER G., BARTENSCHLAGER H., GELDERMANN H., 2000 – Traits values of growth, carcass and meat quality in Wild Boar, Meishan and Pietrain pigs as well as thair crossbred generations. Journal of Animal Breeding and Genetics 117, 189-202.
• 23. OTT J., 1992 – Strategies for characterizing highly polymorphic markers in human gene mapping. American Journal of Human Genetics 51, 283-290.
• 24. PIERZCHAŁA M., KORWIN-KOSSAKOWSKA A., ZWIERZCHOWSKI L., ŁUKASZEWICZ M.,ZIĘBA G., KURYŁ J., 1999 – HaeII and MspI polymorphism of growth hormone gene in pigs and its association with production traits. Czech Journal of Animal Science 44, 441-445.
• 25. REMESAR X., RAFECAS I., FERNANDEZ-LOPEZ J.A., ALEMANY M., 1997 – Leptin. Medicinal Research Reviews 17, 225-234.
• 26. SOUMILLION A., ERKENS J.H.F., LENSTRA J.A., RETTENBERGER G., TE PAS M.F.W.,1997 – Genetic variation in the porcine myogenin gene locus. Mammalian Genome 8, 564-568.
• 27. STRATIL A., KOPEČNY m., 1999 – Genomic organization, sequence and polymorphism of the porcine myostatin (GDF8; MSTN) gene. Animal Genetics 30, 468-470.
• 28. STRATIL A., PEELMAN L., VAN POUCKE M., ČEPICA S., 1997 – A HinfI PCR-RFLP at the porcine leptin (LEP) gene. Animal Genetics 28, 371-372.
• 29. TE PAS M.F.W., HARDERS F.L., A., BORN L., BUIST W., MEUWISSEN T.H.E., 1999 – Genetic variation at the porcine MYF-5 locus. Lack of association with meat production traits. Mammalian Genome 10, 123-127.
• 30. TE PAS M.F.W., VISSCHER A.H., 1994 – Genetic regulation of meat production by embryonic muscle formation – a review. Journal of Animal Breeding and Genetics 111, 404-412.
• 31. TIKHONOV V.N., BOBOVICH V.E., 1997 – Peculiarities of wild and domestic European and Asian pigs. Some aspects of application for gene mapping of markers of pre- and postnatal viability and for conservation genofound. Proceedings of the International Conference on Animal Biotechnology,Peking (People’s Republic of China), pp. 254-258.
• 32. WATANOBE T., OKUMURA N., ISHIGURO N., NAKANO M., MATSUI A., SAHARA S.,KOMATSU M., 1999 - Genetic relationship and distribution of the Japanese wild boar (Sus scrofa laucomystax) and Ryukyu wild boar (Sus scrofa riukiuanus) analysed by mitochondrial DNA. Molecular Ecology 8, 1509-1512.
• 33. YUE G., SCHRÖFFEL J. JR, MOSER G., BARTENSCHLAGER H., REINER G., GELDERMANN H., 2003 – Linkage and QTL mapping for Sus scrofa chromosome 12. Journal of Animal Breeding and Genetics 120 (Suppl. 1), 95-102.