Synergistic effect of DFMO and 2,4-D on regeneration of Tabernaemontana persicariaefolia Jacq in vitro

• Autorzy: Kodja H , Robene-Soustrade I. , Figier J.
• Języki publikacji: EN

Abstrakty

EN

Callus cultures of Tabernaemontana persicariaefolia, (Apocynaceae), an endangered species endemic to the Mascarene Islands, were established from leaf explants on MS medium containing either 5 mg·l⁻¹ 2,4-D and 0.5 mg·l⁻¹ BA or 5 mg·l⁻¹ 2,4-D, 0.5 mg·l⁻¹ BA and 200 mg·l⁻¹ DFMO. Histological studies showed regenerating nodules resembling globular embryos in calli after 4 weeks on the DFMO medium. Green shoot formation was achieved by sequential subculture of the induced calli on media with gradually decreasing 2,4-D concentrations (5→1→0 mg·l⁻¹). Regeneration was greatly stimulated in the presence of DFMO. The first emergence of shoots occured 3 weeks earlier than in untreated callus cultures.

Słowa kluczowe

EN

polyamine; Apocynaceae; regeneration; in vitro; Tabernaemontana persicariaefolia; callus induction

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 1997
• Tom: 19
• Numer: 3
• Opis fizyczny: p.359-366,fig.

Twórcy

autor

Kodja H

• Universite de la Reunion, 15, avenue Rene Cassin, BP 7151, 97715 St.Denis Messag. cedex 9, Ile de La Reunion, France

autor

Robene-Soustrade I.

autor

Figier J.

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