New genetic map of rye composed of PCR-based molecular markers and its alignment with reference map of the DS2 x RXL10 intercross

• Autorzy: Milczarski P , Banek-Tabor A. , Lebiecka K. , Stojalowski S. , Myskow B. , Masojc P.
• Języki publikacji: EN

Abstrakty

EN

A new genetic map of rye, developed by using the 541 × Otl-3 F₂ intercross, consists of 148 marker loci, including 99 RAPDs, 18 SSRs, 14 STSs, 9 SCARs and 7 ISSRs, and spans the distance of 1401.4 cM. To the 7 rye chromosomes, 8 linkage groups were assigned and compared with the reference map of the DS2 × RXL10 F₂ intercross by using 24 common markers. The 2 combined maps contain altogether 611 marker loci (70-109 per chromosome) and constitute a substantial source of information useful for further genomic studies in rye. From 21 to 37 RAPD marker loci are distributed randomly along each chromosome length and their total number for all 7 rye chromosomes is 177. This abundance of RAPD marker loci in the rye genetic map can be exploited for development of SCARs in regions containing important genes or QTL.

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2007
• Tom: 48
• Numer: 1
• Opis fizyczny: p.11-24,fig.,ref.

Twórcy

autor

Milczarski P

• University of Agriculture in Szczecin, Slowackiego 17, 71-434 Szczecin, Poland

autor

Banek-Tabor A.

autor

Lebiecka K.

autor

Stojalowski S.

autor

Myskow B.

autor

Masojc P.

Bibliografia

• Bednarek PT, Masojć P, Lewandowska R, Myśków B, 2003. Saturating rye genetic map with amplified fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) markers. JAppl Genet 44: 21-33.
• Börner A, Korzun V, 1998. A consensus linkage map of rye (Secale cereale L.) including 374 RFLPs, 24 isozymes and 15 gene loci. Theor Appl Genet 97: 1279-1288.
• Camacho MV, Matos M, Gonzales C, Perez-Flores V, Pernauta B, Pinto-Carnida O, Benito C. 2005. Secale cereale inter-microsatellites (SCIMs): chromosomal location and genetic inheritance. Genetica 123: 303-311.
• Devos KM, Atkinson MD, Chinoy CN, Francis HA, Harcourt RL, Koebner RMD, et al. 1993. Chromosomal rearrangements in the rye genome relative to that of wheat. Theor Appl Genet 85: 673-680.
• Gonzales C, Camacho MV, Benito C, 2002. Chromosomal location of 46 new RAPD markers in rye (Secale cereale L.). Genetica 115: 205-211.
• Hackauf B, Wehling P, 2002. Identification of microsatellite polymorphism in an expressed portion of the rye genome. Plant Breed 121: 17-25.
• Hackauf B, Wehling P, 2003. Development of microsatellite markers in rye: map construction. Plant Breed Seed Sci 48: 143-151.
• Khlestkina EK, Than MHM, Pestsova EG, Röder MS, Malyshev SV, Korzun V, Börner A, 2004. Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags. Theor Appl Genet 109: 725-732.
• Korzun V, Malyshev S, Kartel N, Westermann T, Weber WE, Börner A, 1998. A genetic linkage map of rye (Secale cereale L.). Theor Appl Genet 96: 203-208.
• Korzun V, Malyshev S, Voylokov AV, Börner A, 2001. A genetic map of rye (Secale cereale L.) combining RFLP, isozyme, protein, microsatellite and gene loci. Theor Appl Genet 102: 709-717.
• Lander ES, Green P, Abramson J, Barlow A, Daly MJ, Lincoln SE, Newburg L, 1987. MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1: 174-181.
• Loarce Y, Hueros G, Ferrer E, 1996. A molecular linkage map of rye. Theor Appl Genet 93: 1112-1118.
• Ma X-F, Wanous MK, Houchins K, Rodriguez Milla MA, Goicoechea PG, Wang Z, et al. 2001. Molecular linkage mapping in rye (Secale cereale L.). Theor Appl Genet 102: 517-523.
• Mago R, Miah H, Lawrence GJ, Wellings CR, Spielmeyer W, Bariana HS, et al. 2005. High-resolution mapping and mutation analysis separate the rust resistance genes Sr31, Lr26 and Yr9 on the short arm of rye chromosome 1. Theor Appl Genet 112: 41-50.
• Mago R, Spielmeyer W, Lawrance GJ, Lagudah ES, Ellis JG, Pryor A, 2002. Identification and mapping of molecular markers linked to rust resistance genes located on chromosome IRS of rye using wheat-rye translocation lines. Theor Appl Genet 104: 1317-1324.
• Malyshev SV, Kartel NA, Voylokov AV, Korzun V, Börner A, 2003. Comparative analysis of QTLs affecting agronomical traits in rye and wheat. EWAC-Newsl 12: 120-122.
• Masojć P, Gale MD, 1990. The factor modifying α-amylase isozyme pattern from rye endosperm is an endogenous α-amylase inhibitor. Hereditas 113: 151-155.
• Masojć P, Myśków B, Milczarski P, 2001. Extending a RFLP-based genetic map of rye using random amplified polymorphic DNA (FLAPD) and isozyme markers. Theor Appl Genet 102: 1273-1279.
• Masojć P, Milczarski P, 2005. Mapping QTLs for alpha-amylase activity in rye grain. J Appl Genet 46: 115-123.
• Myśków B, Masojć P, Banek-Tabor A, Szołkowski A, 2001. Genetic diversity of rye inbred lines evaluated by RAPD analysis. J Appl Genet 42: 1-14.
• Nagy ED, Lelley T, 2003. Genetic and physical mapping of sequence-specific amplified polymorphic (SSAP) markers on the 1RS chromosome arm of rye in a wheat background. Theor Appl Genet 107: 1271-1277.
• Parida SK, Kumar KAR, Dalai V, Singh NK, Mohapatra T, 2006. Unigene derived microsatellite markers for the cereal genomes. Theor Appl Genet 112: 808-817.
• Philipp U, Wehling P, Wricke G, 1994. A linkage map of rye. Theor Appl Genet 88: 243-248.
• Rozen S, Skaletsky HJ, 2000. Primer 3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S, eds. Bioinformatics methods and protocols: Methods in molecular biology. Humana Press, Totowa, NJ: 365-386.
• Saal B, Wricke G, 1999. Development of simple sequence repeat markers in rye (Secale cereale L.). Genome 42: 964-972.
• Saal B, Wricke G, 2002. Clustering of amplified fragment length polymorphism markers in a linkage map of rye. Plant Breed 121: 117-123.
• Sambrook J, Russell DW, 2001. Molecular cloning: A laboratory manual, Vol 2. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
• Senft P, Wricke G, 1996. An extended genetic map of rye (Secale cereale L.). Plant Breed 115: 508-510.
• Stojałowski S, Jaciubek M, Masojć P, 2005. Rye SCAR markers for male fertility restoration in the P cytoplasm are also applicable to marker-assisted selection in the C cytoplasm. J Appl Genet 46: 371-373.
• Stojałowski S, Milczarski P, Masojć P, 2004. Usefulness of ISSR markers for identification of inbred lines and mapping of rye genome. Biul Inst Hod Aklim Rosl 231:237-246.
• Stracke S, Schilling AG, Förster J, Weiss C, Glass C, Miedaner T, Geiger HH, 2003. Development of PCR-based markers linked to dominant genes for male-fertility restoration in Pampa CMS of rye (Secale cereale L.) Theor Appl Genet 106: 1184-1190.
• Westermeier R, 1993. Electrophoresis in practice. VCH, Weinheim 107-108.