Development of photosynthetic apparatus and respiration in pea seedlings during greening as influenced by toxic concentration of lead

• Autorzy: Lukaszek M , Poskuta J.W.
• Języki publikacji: EN

Abstrakty

EN

Detached leaves of 14 day-old dark-grown pea seedlings were immersed with their cut ends either in water (control) or in 20 mM Pb(NO₃)₂ solution. They were exposed to continuous illumination during 24 and 48 h. The formation of PSII primary photochemistry in thylakoids was determined in vivo by measuring changes in values of parameters of chlorophyll a fast fluorescence kinetics: Fo, Fm, Fv, Fv/Fm and t 1/2. The amount of lead accumulation in leaves, content of chlorophylls and carotenoids and rates of CO₂ uptake in light and evolution in darkness (Pn-net photosynthesis and DR - dark respiration respectively) were determined. It has been found that with the exception of Fo, values of Fv, Fm and Fv/Fm were reduced by Pb²⁺. The values of t 1/2 were significantly larger in Pb²⁺ treated leaves. Decrease in the chlorophyll a fluorescence parameters was paralleled with the strong inhibition by this metal the biosynthesis of chlorophyll a and b but less of the carotenoids. Pb²⁺ drastically reduced Pn but had a stimulatory action on DR after 24 h and small inhibition of DR after 48 h exposure of leaves to this metal. As a consequence, after 48 h of greening the ratio of DR/Pn of control leaves was 0.45 whereas in Pb²⁺ treated leaves 2.7. It is proposed that DR in leaves plays a protective role against damage of Pn by Pb²⁺. Protection can be due to the supply the respiratory derived reductant and ATP to carry out cell metabolism upon reduced photosynthesis.

Słowa kluczowe

EN

photosynthetic apparatus; photosystem II; pea; chlorophyll; lead; Pisum sativum; photosynthesis; respiration

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 1998
• Tom: 20
• Numer: 1
• Opis fizyczny: p.35-40,fig.

Twórcy

autor

Lukaszek M

• University of Warsaw, Krakowskie Przedmiescie 26-28, 00-927 Warsaw, Poland

autor

Poskuta J.W.

Bibliografia

• Baker, N. R. and Butler W. L. 1976. Development of the primary photochemical apparatus of photosynthesis during greening of etiolated beaves. Plant Physiol. 58, 526–529.
• Baker, N. R. 1991. A possible role of photosystem II in environmental perturbation of photosynthesis. Physiol. Plant. 81, 536–570.
• Ballantyne, D. J. 1992. Effect of fluoride on photosynthetic and respiration rates in azalea (Rhododendron) cultivars, Photosynthetica 27, 637–641.
• Bjorkman, O. and Demmig B. 1987. Photon yield of oxygen evolution and chlorophyll fluorescence characteristics at 77 K among vascular plants of diverse origins. Planta 170, 489–504.
• Burzyński, M. and Buczek J. 1994a. The influence of Cd, Pb, Cu and Ni on NO− 3 uptake by cucumber seedlings. I. Nitrate uptake and respiration of cucumber seedlings roots treated with Cd, Pb, Cu and Ni. Acta Physiol. Plantarum 16, 291–296.
• Burzyński, M. and Buczek J. 1994b. The influence of Cd, Pb, Cu and Ni on NO− 3 uptake by cucumber seedlings. II. In vitro and in vivo effects of Cd, Cu, Pb and Ni on the plasmalemma ATPase and oxidoreductase from cucumber seedlings roots. Acta Physiol. Plantarum 16, 297–302.
• Genty, B. J., Briantais M. and Baker N. R. 1989. The relationship between the quantum yield of photosynthetic electron transport and quenches of chlorophyll fluorescence. Biochim. Biophys. Acta 990, 87–92.
• Greger, M. and Ögren E. 1991. Direct and indirect effects of Cd2+ on photosynthesis in sugar beet (Beta vulgaris). Physiol. Plantarum 83, 129–135.
• Havaux, M. and Lannoye R. 1985. In vivo chlorophyll fluorescence and delayed light emission as rapid screening techniques for stress tolerance in crop plants. Z. Pflanzenhchtg. 95, 1–13.
• Hurry, V., Tobiaeson M., Krömer S., Gardeström P. and Öquist G. 1995. Mitochondria contribute to increased photosynthetic capacity of leaves of winter rye (Secale cereale L.) following cold-hardening. Plant Cell Environ. 18, 69–76.
• Koeppe, D. E. and Miller R. J. 1970. Lead effects on corn mitochondrial respiration. Science 167, 1376–1377.
• Krause, G. H. and Santarius K. A. 1975. Relative thermostability of the chloroplast envelope. Planta 127, 285–299.
• Krause, G. H. and Weis E. 1984. Review: Chlorophyll fluorescence as a tool in plant physiology. II. Interpretation of fluorescence signals. Photosynth. Res. 5, 139–157.
• Krause, G. H. and Weis E. 1988. The photosynthetic apparatus and chlorophyll fluorescence. An introduction. In: Lichtenthaler H. K. (ed): Application of chlorophyll fluorescence in photosynthesis research, stress physiology, hydrobiology and remote sensing. Kluwer Academic Publishers, The Netherlands, 3–11.
• Krömer, S. and Heldt H. 1991. Respiration of pea leaf mitochondria and redox transfer between the mitochondrial and extramitochondrial compartment. Biochem. Biophys. Acta 1057, 42–50.
• Krömer, S., Malmberg G. and Gardeström P. 1993. Mitochondrial contribution to photosynthetic metabolism. A study with barely (Horedum vulgare L.) leaf protoplasts at different light intensities and CO2 concentrations. Plant Physiol. 102, 947–955.
• Lamoreaux, R. J. and Chaney W. R. 1978. The effect of cadmium on net photosynthesis, transpiration, and dark respiration of excised silver maple leaves. Physiol. Plant. 43, 231–236.
• Lichtenthaler, H. K. and Wellburn R. R. 1983. Determination of total carotenoids and chlorophylls a and b in leaf extracts in different solvents. Biochem. Soc. Trans. 603, 591–592.
• Mathis, P. and Paillotin G. 1981. Primary processes of photosynthesis. In: M. D. Hatch and N. K. Boardman (eds) The biochemistry of Plants 8, 97–161, Academic Press, New York, London.
• Moustakas, M., Lanaras T., Symeonidis L. and Karataglis S. 1994. Growth and some photosynthetic characteristics of field grown Avena sativa under copper and lead stress. Photosynthetica 30, 389–396.
• Oberhuber, W. and Edwards G. E. 1993. Temperature dependence of the linkage of quantum yield of PSII to carbon dioxide fixation in C-4 and C-3 plants. Plant. Physiol. 101, 507–512.
• Öquist, G. and Wass R. 1988. A portable, microprocessor operated instrument for measuring chlorophyll fluorescence kinetics in stress physiology. Physiol. Plantarum 73, 211–217.
• Parys, E., Romanowska E., Lechowicz W. and Poskuta J. 1989. Postillumination burst of CO2 in maize and reed grass leaves determined in a closed gas exchange system. Plant Physiol. Biochem. 27, 27–34.
• Parys, E., Romanowska E., Siedlecka M. and Poskuta J. W. 1997. The effect of lead on photosynthesis and respiration in detached pea leaves and in their mesophyll protoplasts (in press).
• Poskuta, J., Mikulska M., Faltynowicz M., Bielak B. and Wróblewska B. 1974. The effect of oxygen concentration on the development of photosynthetic capacity by etiolated bean seedlings upon illumination. Z. Pflanzenphysiol. 73, 387–393.
• Poskuta, J. W., Parys E. and Romanowska E. 1987. The effects of lead on the gaseous exchange and photosynthetic carbon metabolism of pea sedlings. Acta Soc. Bot. Pol. 56, 127–137.
• Poskuta, J. W., Parys E., Romanowska E., Gajdzis-Gujdan H. and Wróblewska B. 1988. The effect of lead on photosynthesis 14C distribution among photoassimilates and transpiration of maize seedlings. Acta Soc. Bot. Pol. 57, 149–155.
• Poskuta, J. W. and Waclawczyk-Lach E. 1995. In vivo responses of primary photochemistry of photosystem II and CO2 exchange in light and in darkness of tall fescue genotypes to lead toxicity. Acta Physiol. Plantarum 17, 233–240.
• Poskuta, J. W., Parys E. and Romanowska E. 1996. Toxicity of lead to photosynthesis, accumulation of chlorophyll, respiration and growth of Chlorella pyrenoidosa. Protective role of dark respiration. Acta Physiol. Plant. 18, 165–171.
• Sgardelis, S., Cook C. M., Pantis J. D. and Lanaras T. 1994. Comparison of chlorophyll fluorescence and some heavy metal concentrations in Sonchus spp. and Taraxacum spp. along urban pollution gradient. Sci. Total Environ. 158, 157–164.
• Schreiber, U. and Berry J. A. 1977. Heat-induced changes of chlorophyll fluorescence in intact leavs correlated with damage of the photosynthetic apparatus. Planta 136, 233–238.
• Woźny, A. and Krzesłowska M. 1993. Plant cell responses to lead. Acta Soc. Bot. Pol. 62, 101–105.
• Wożny, A. Schneider J. and Gwóźdź E. A. 1995. The effect of lead and kinetin on greening barely leaves. Biol. Plant. 37, 542–552.
• Wróblewska, B., Mostowska A. and Poskuta J. W. 1994a. The effect of oxygen concentration on the chloroplast development, chlorophyll synthesis and starch accumulation in etiolated bean seedlings upon illumination. Environ. Exp. Bot. 34, 153–163.
• Wróblewska, B., Siedlecka M. and Poskuta J. W. 1994b. The action of 2, 21 and 100% of oxygen on primary photochemistry of photosystem II (PS II), biosynthesis of pigments and carbon dioxide fixation by etiolated bean leaves during greening. Proc. Royal Soc. of Edinburgh 102B, 75–80.