High levels of bulky DNA adducts in human sperm correlate with impaired fertility

• Autorzy: Horak S. , Polanska J. , Widlak P.
• Języki publikacji: EN

Abstrakty

EN

Progressive decline in fertility and sperm quality has been reported over the last few decades, especially in industrialized nations. It has been proposed that exposure to factors that induce damage in DNA of spermatogenic cells may significantly contribute to impaired fertility. Here, the 32P-postlabelling method was used to analyze the levels of bulky DNA adducts in sperm cells in a group of 179 volunteers, either healthy subjects or patients with an impaired fertility. The levels of DNA adducts were 1.35-fold higher in the infertile group as compared to healthy individuals (P = 0.012). Similarly, a significant negative correlation between the levels of DNA adducts and measures of semen quality (sperm concentration and motility) has been observed (P < 0.001). In addition, the levels of bulky DNA adducts in sperm cells positively corre­lates with amounts of leukocytes in semen, which were significantly higher in semen of infertile subjects.

Słowa kluczowe

EN

sperm quality; man; fertility; spermatozoon; human sperm; DNA damage

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2003
• Tom: 50
• Numer: 1
• Opis fizyczny: p.197-203,fig.

Twórcy

autor

Horak S.

• Silesian Medical Academy, Bytom, Poland

autor

Polanska J.

autor

Widlak P.

Bibliografia

• Beach, AC, Gupta, RC. (1992) Carcinogenesis.; 13: 1053-74.
• Gallagher JE, Vine MF, Schramm MM, Lewtas J, George MH, Hulka B, Everson RB. (1993) Cancer Epidemiol BiomarkersPrev.; 2: 581-5.
• Ginsberg GL, Atherholt B. (1989) Carcinogenesis.; 10: 673-9.
• Giwercman A, Bonde JP. (1998) EndocrinolMetab Clin North Am.; 27: 807-30.
• Grzybowska E, Hemminki K, Szeliga J, Chorazy M. (1993) Carcinogenesis.; 14: 2523-6.
• Gupta RC, Reddy MV, Randerath K. (1982) Carcinogenesis.; 3: 1081-92.
• Harris CC. (1986) Environ Health Perspect.; 62: 185-91.
• Hemminki K, Grzybowska E, Widlak P, Chorazy M. (1996) Acta Biochim Polon.; 43: 305-12. Hruska KS, Furth PA, Seifer DB, Sharara FI, Flaws JA. (2000) Clin Obstet Gynecol.; 43: 821-29. Lahdetie J. (1995) J Occup Environ Med.; 37: 922-30.
• Lewtas J, Mumford J, Everson RB, Hulka B, Wilcosky T, Kozumbo W, Thompson C, George M, Dobias L, Sram R, Li X, Gallagher JE. (1993) Environ Health Perspect.; 99: 89-97.
• Perera FP, Hemminki K, Grzybowska E, Motykiewicz G, Michalska J, Santella RM, Young TL, Dickey C, Brandt-Rauf P, DeVivo I, Blaner W, Tsai WY, Chorazy M. (1992) Nature.; 360: 256-8.
• Randerath K, Randerath E, Zhou GD, Li D. (1999) MutatRes.; 424: 183-94.
• Roy D, Liehr JG. (1999) Mutat Res.; 424: 107-15.
• Tas S, Lauwerys R, Lison D. (1996) Crit Rev Toxicol.; 26: 261-307.
• Vine MF. (1996) Int J Androl.; 19: 323-37.
• Widlak P, Grzybowska E, Hemminki K, Santella, R, Chorazy M. (1996) Chem Biol Interact.; 99: 99-107. World Health Organization (1987) Fertil Steril.; 47: 964-8.
• World Health Organization (1992) WHO laboratory manual for the examination of human semen and sperm-cervical mucus interactions. 3rd edn. Cambridge University Press, Cambridge.
• Zenzes MT, Bielecki R, Reed TE. (1999) Fertil Steril.; 72: 330-5.