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1997 | 44 | 4 |
Tytuł artykułu

Phosphorylation of sucrose synthase from maize seedlings

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Two isoforms of sucrose synthase (SS1 and SS2) from maize (Zea mays, var. Mona) seedlings co-purified with a calcium and phospholipid dependent protein kinase. The enzymatic preparation obtained gave a positive reaction with the antibody against mammalian protein kinase C. Maize sucrose synthase was phosphorylated by the endogenous protein kinase. Also, mammalian protein kinases (protein kinase C and protein kinase A) were able to phosphorylate the 86 kDa subunit of sucrose synthase. When excised seedlings were fed [32P]orthophosphate, sucrose synthase was also phosphorylated. Microsequencing of in vivo labelled enzyme has shown phosphorylation of Ser-15 in SS2. The present work provides evidence that maize sucrose synthase is the physiological substrate of the endogenous calcium and phospholipid dependent protein kinase(s).
Wydawca
-
Rocznik
Tom
44
Numer
4
Opis fizyczny
p.809-817,fig.
Twórcy
autor
  • Biomedical Centre, Box 575, S-75123 Uppsala, Sweden
autor
autor
autor
autor
Bibliografia
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-article-956d8764-53ab-4ed7-9e77-2514ba781b5d
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