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1993 | 40 | 4 |
Tytuł artykułu

Effect of glucose and deoxyglucose on cytoplasmic concentration of free Ca2 in Ehrlich ascites tumour: studies on single cells

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Concentration of free cytoplasmic Ca2+ ([Ca2+]i) in Ehrlich ascites tumour cells loaded with fura-2 was measured in single cells applying a video imaging system. In resting cells [Ca2+li amounted to 60 - 340 nM and was increased after addition of 10 mM D-glucose or D-2-deoxyglucose by 80 - 200 nM. This increase occurred within 30 - 60 s following addition of the sugars and lasted for several minutes. Pretreatment of the cells with thapsigargin resulted in a much smaller [Ca2+]i increase after addition of glucose or deoxyglucose and, vice versa, thapsigargin added after the sugars mobilized less Ca2+ than when added before. A possible relation of the [Ca2+]i rise evoked by glucose and deoxyglucose to the Crabtree effect is discussed.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
40
Numer
4
Opis fizyczny
p.539-544,fig.
Twórcy
autor
  • M.Nencki Institute of Experimental Biology, L.Pasteura 3, 02-093 Warsaw, Poland
autor
autor
autor
autor
Bibliografia
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  • 4. Landry, Y. & Lehninger, A.L. (1976) Transport of calcium ions by Ehrlich ascites-tumour cells. Biochem.]. 158,427 - 438.
  • 5. Hinnen, R., Miyamoto, H. & Racker, E. (1979) Ca2+ translocation in Ehrlich ascites tumor cells. /. Membr. Biol. 49,309 - 324.
  • 6. Cittadini, A., Bossi, D., Dani, A.M., Calviello, G., Wolf, F. & Terranova, T. (1981) Lack of effect of the Ca2+ ionophore A23187 on tumour cells. Biochim. Biophys. Acta 645,177 -182.
  • 7. Cittadini, A., Dani, A.M., Wolf, P., Bossi, D. & Calviello, G. (1982) Calcium permeability of Ehrlich ascites tumour cell plasma membrane in vivo. Biochim. Biophys. Acta 686, 27 - 35.
  • 8. Arslan, P., Di Virgilio, F., Beltrame, M., Tsien, R.Y. & Pozzan, T. (1985) Cytosolic Ca2+ homeostasis in Ehrlich and Yoshida carcinomas. A new, membrane-permeant chelator of heavy metals reveals that these ascites tumor cell lines have normal cytosolic free Ca2+. J. Biol. Chem. 260, 2719 - 2727.
  • 9. Teplova, V.V., Bogucka, K., Czyż, A., Evtodienko, Yu.V., Duszynski, J. & Wojtczak, L. (1993) Effect of glucose and deoxyglucose on cytoplasmic [Ca *] in Ehrlich ascites tumor cells. Biochem. Biophys. Res. Commun. in press.
  • 10. Bogucka, K., Wroniszewska, A., Bednarek, M., Duszyński, J. & Wojtczak, L. (1990) Energetics of Ehrlich ascites mitochondria: membrane potential of isolated mitochondria and mitochondria within digitonin-permeabilized cells. Biochim. Biophys. Acta 1015,503 - 509.
  • 11. Dubyak, G.R. (1986) Extracellular ATP activates polyphosphoinositide breakdown and Ca2+ mobilization in Ehrlich ascites tumor cells. Arch. Biochem. Biophys. 245,84 - 95.
  • 12. Grynkiewicz, G., Poenie, M. & Tsien, R.Y. (1985) A new generation of Ca2+ indicators with greatly improved fluorescence properties. /. Biol. Chem. 260,3440 - 3450.
  • 13. Thastrup, O., Cullen, P.J., Dr0bak, B.K., Hanley, M R. & Dawson, A.P. (1990) Thapsigargin, a tumor promoter, discharges intracellular Ca* stores by specific inhibition of the endoplasmic Ca2+-ATPase. Proc. Natl. Acad. Sci. U.S.A. 87, 2466 - 2470.
  • 14. Mick, G.J., Lee, J. & McCormick, K.L. (1991) Selective stimulation of in situ intermediary metabolism by free calcium in permeabilized rat adipocytes. Biochim. Biophys. Acta 1133,73 - 80.
  • 15. Thorne, R.F.W. & Bygrave, F.L. (1973) Energy-linked functions of tightly coupled mitochondria isolated from Ehrlich ascites tumor cells. Cancer Res. 33,2562 - 2567.
  • 16. Thome, R.F.W. & Bygrave, F.L. (1974) The role of mitochondria in modifying the cellular ionic environment. Calcium-induced respiratory activities in mitochondria isolated from various tumour cells. Biochetn. J. 144,551 - 558.
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  • 18. Villalobo, A. & Lehninger, A.L. (1980) Inhibition of oxidative phosphorylation in ascites tumor mitochondria and cells by intramitochondrial Ca2+. /. Biol. Chain. 255,2457 - 2464.
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  • 20. Kun, E., Talalay, P. & Williams-Ashman, H.G. (1951) Studies on the Ehrlich ascites tumor. I. The enzymic and metabolic activities of the ascitic cells and the ascitic plasma. Cancer Res. 11,855-863.
  • 21. Chance, B. & Hess, B. (1956) On the control of metabolism in ascites tumor cell suspensions. Ann. N. Y. Acad. Sci. 63,1008 - 1016.
  • 22. Ibsen, K.H., Coe, E.L. & McKee, R.W. (1958) Interrelationships of metabolic pathways in the Ehrlich ascites carcinoma cells. I. Glycolysis and respiration (Crabtree effect). Biochim. Biophys. Acta 30,384 - 400.
  • 23. Wu, R. & Racker, E. (1959) Regulatory mecha­nisms in carbohydrate metabolism. IV. Pasteur effect and Crabtree effect in ascites tumor cells. J. Biol. Chem. 234,1036 -1041.
  • 24. Ibsen, K.H. (1961) The Crabtree effect: a review. Cancer Res. 21,829-841.
  • 25. Lardy, H.A. & Phillips, PH. (1941) The interrelation of oxidative and glycolytic proces­ses as source of energy for bull spermatozoa. Am. J. Physiol. 133,602 - 609.
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  • 29. Guppy, M., Greiner, E. & Brand, K. (1993) The Crabtree effect and an endogenous fuel in the energy metabolism of resting and proliferating thymocytes. Eur. J. Biochem. 212,95 - 99.
Typ dokumentu
Bibliografia
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Identyfikator YADDA
bwmeta1.element.agro-article-953e2da8-8f08-4677-b4de-f6be1d825dda
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