Lack of correlation between X region spa polymorphism and virulence of methicillin resistant and methicillin sensitive Staphylococcus aureus strains

• Autorzy: Kurlenda J. , Grinholc M. , Szweda P.
• Języki publikacji: EN

Abstrakty

EN

Staphylococcus aureus is an etiological factor of severe infections in both hospital and ambulatory environments. As methicillin resistant Staphylococcus aureus strains spread quickly across healthcare centers resulting in life-threatening infections with increased mortality, they are considered more virulent than MSSA strains. Protein A, encoded by the spa gene, is one of the virulence factors involved in the staphylococcal pathogenesis. It has been suggested that the number of 24-bp tandem repeat units along the X region of the spa gene correlates with the virulence level of the strains. The current work analyzed the relationships between the virulence of MRSA and MSSA strains with region X polymorphism. No obvious correlation was observed.

Słowa kluczowe

EN

polymorphism; protein A; virulence; methicillin resistance; Staphylococcus aureus; methicillin-resistant strain; methicillin-sensitive strain; infection; human disease

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2010
• Tom: 57
• Numer: 1
• Opis fizyczny: p.135-138,ref.

Twórcy

autor

Kurlenda J.

• Department of Clinical Bacteriology in Provincial Hospital, Koszalin, Poland

autor

Grinholc M.

autor

Szweda P.

Bibliografia

• Aathithan S, Dybowski R, French GL (2001) Highly epidemic strains of methicillin-resistant Staphylococcus aureus not distinguished by capsule formation, protein A content or adherence to HEp-2 cells. Eur J Clin Microbiol Infect Dis 20: 27-32. 
• Bignardi GE, Woodford N, Chapman A, Johnson AP, Speller DCE (1996) Detection of the mec-A gene and phenotypic detection of resistance in Staphylococcus aureus isolates with borderline or low-level methicillin resistance. J Antimicrob Chemother 37: 53-63. 
• Collins RJ (2007) Community-acquired methicillin-resistant Staphylococcus aureus in a group home setting. Consult Pharm 22: 763-767. 
• Deurenberg RH, Vink C, Kalenic S, Friedrich AW, Bruggeman CA, Stobberingh EE (2007) The molecular evolution of methicillin-resistant Staphylococcus aureus. Clin Microbiol Infect 13: 222-235. 
• El-Sayed A, Alber J, Lammler C, Bonner B, Huhn A, Kaleta EF, Zschock M (2005) PCR-based detection of genes encoding virulence determinants in Staphylococcus aureus from birds. J Vet Med B Infect Dis Vet Public Health 52: 38-44. 
• Frenay HM, Bunschoten AE, Schouls LM, van Leeuwen WJ, Vandenbroucke-Grauls CM, Verhoef J, Mooi FR (1996) Molecular typing of methicillin-resistant Staphylococcus aureus on the basis of protein A gene polymorphism. Eur J Clin Microbiol Infect Dis 15: 60-64. 
• Frenay HM, Theelen JP, Schouls LM, Vandenbroucke-Grauls C, Verhoef J, van Leeuwen WJ, Mooi FR (1994) Discrimination of epidemic and nonepidemic methicillin-resistant Staphylococcus aureus strains on the basis of protein A gene polymorphism. J Clin Microbiol 32: 846-847. 
• Gosbell IB (2005) Epidemiology, clinical features and management of infections due to community methicillin-resistant Staphylococcus aureus (cMRSA). Intern Med J 35 (Suppl 2): S120-S135. 
• Grinholc M, Węgrzyn G, Kurlenda J (2007) Evaluation of biofilm production and prevalence of the icaD gene in methicillin resistant and methicillin-sensitive Staphylococcus aureus strains isolated from patients with nosocomial infections and carriers. FEMS Immunol Med Microbiol 50: 375-379. 
• Huang YH, Tseng SP, Hu JM, Tsai JC, Hsueh PR, Teng LJ (2007) Clonal spread of SCCmec type IV methicillin-resistant Staphylococcus aureus between community and hospital. Clin Microbiol Infect 13: 717-724. 
• Jakubczak A, Szweda P, Lukaszewska K, Kur J (2007) Molecular typing of Staphylococcus aureus isolated from cows with mastitis in the east of Poland on the basis of polymorphism of genes coding protein A and coagulase. Pol J Vet Sci 10: 199-205. 
• Kuint J, Barzilai A, Regev-Yochay G, Rubinstein E, Keller N, Maayan-Metzger A (2007) Comparison of community-acquired methicillin-resistant Staphylococcus aureus bacteremia to other staphylococcal species in a neonatal intensive care unit. Eur J Pediatr 166: 319-325. 
• Kurlenda J, Grinholc M, Jasek K, Wegrzyn G (2007) RAPD typing of methicillin-resistant Staphylococcus aureus: a 7-year experience in a Polish hospital. Med Sci Monit 13: MT13-MT18. 
• Kurlenda J, Grinholc M, Krzyszton-Russjan J, Wisniewska K (2009) Epidemiological investigation of nosocomial outbreak of staphylococcal skin diseases in neonatal ward. Antonie Van Leeuwenhoek 95: 387-394. 
• Kurlenda J, Grinholc M, Węgrzyn G (2008) Presence of cna, emp and pls genes and pathogenicity of methicillin-resistant Staphylococcus aureus strains. World J Microbiol Biotechnol 24: 591-594.
• Kuzma K, Malinowski E, Lassa H, Klossowska A (2005) Analysis of protein A gene polymorphism in Staphylococcus aureus isolates from bovine mastitis. Bull Vet Inst Pulawy 49: 41-44.
• Ladhani S, Garbash M (2005) Staphylococcal skin infections in children: rational drug therapy recommendations. Paediatr Drugs 7: 77-102. 
• Lee LY, Liang X, Hook M, Brown EL (2004) Identification and characterization of the C3 binding domain of the Staphylococcus aureus extracellular fibrinogen-binding protein (Efb). J Biol Chem 279: 50710-50716. 
• Melzer M, Eykyn SJ, Gransden WR, Chinn S (2003) Is methicillin-resistant Staphylococcus aureus more virulent than methicillin-susceptible S. aureus? A comparative cohort study of British patients with nosocomial infection and bacteremia. Clin Infect Dis 37: 1453-1460. 
• Nashev D, Toshkova K, Salasia SI, Hassan AA, Lammler C, Zschock M (2004) Distribution of virulence genes of Staphylococcus aureus isolated from stable nasal carriers. FEMS Microbiol Lett 233: 45-52. 
• Performance standards for antimicrobial susceptibility testing; eighteen informational supplement (2008) CLSI M100-S18, vol. 28, No. 1.
• Sabat A, Krzyszton-Russjan J, Strzalka W, Filipek R, Kosowska K, Hryniewicz W, Travis J, Potempa J (2003) New method for typing Staphylococcus aureus strains: multiple-locus variable-number tandem repeat analysis of polymorphism and genetic relationships of clinical isolates. J Clin Microbiol 41 1801-1804. 
• Sola C, Gribaudo G, Vindel A, Patrito L, Bocco JL (2002) Identification of a novel methicillin-resistant Staphylococcus aureus epidemic clone in Cordoba, Argentina, involved in nosocomial infections. J Clin Microbiol 40: 1427-1435. 
• Tang J, Zhou R, Shi X, Kang M, Wang H, Chen H (2008) Two thermostable nucleases coexisted in Staphylococcus aureus: evidence from mutagenesis and in vivo expression. FEMS Microbiol Lett 284: 176-183. 
• Vimercati C, Cremonesi P, Castiglioni B, Pisoni G, Boettcher PJ, Vicenzoni G, Moroni P (2006) Molecular typing of Staphylococcus aureus isolated from cows, goats and sheep with intramammary infection on the basis of gene polymorphisms and toxin genes. J Vet Med B Infect Dis Vet Public Health 53: 423-428. 
• Voyich JM, Braughton KR, Sturdevant DE, Whitney AR, Said-Salim B, Porcella SF, Long RD, Dorward DW, Gardner DJ, Kreiswirth BN, Musser JM, DeLeo FR (2005) Insights into mechanisms used by Staphylococcus aureus to avoid destruction by human neutrophils. J Immunol 175: 3907-3919. 
• Walker J, Borrow R, Edwards-Jones V, Oppenheim BA, Fox AJ (1998) Epidemiological characterization of methicillin-resistant Staphylococcus aureus isolated in the North West of England by protein A (spa) and coagulase (coa) gene polymorphisms. Epidemiol Infect 121: 507-514.