Isolation of the DNA fragment reflecting the open reading frame II of the I-18 C gene of Chironomus tentans by the polymerase chain reaction. II. The technology for the preparation of the template

• Autorzy: Borowicz B P
• Języki publikacji: EN

Abstrakty

EN

The technology for the preparation of the template in the research aimed to isolate the DNA fragment reflecting the Open Reading Frame II of the I-18 C gene is presented i.e. the linearisation of the template by Bam H I digestion, the purification of the template and the estimation of the template concentration.

PL

Przedstawiono technologię przygotowywania matrycy DNA w badaniach zmierzających do izolacji Otwartej Ramy Odczytu II genu I-18 C Chironomus tentans. Technologia ta obejmowała linearyzację DNA matrycy poprzez trawienie Bam H I oraz oczyszczenie matrycy i oznaczenie stężenia matrycowego DNA.

Słowa kluczowe

EN

gene expression; I-18 C gene; Chironomus tentans; polymerase chain reaction; DNA fragment

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 1997
• Tom: 37
• Numer: 1-2
• Opis fizyczny: p.145-148

Twórcy

autor

Borowicz B P

• Institute of Plant Protection, Miczurina 20, 60-318 Poznan, Poland

Bibliografia

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