The differential expression of ribosomal 18S RNA paralog genes from the chaetognath Spadella cephaloptera

• Autorzy: Barthelemy R -M , Grino M. , Pontarotti P. , Casanova J.-P. , Faure E.
• Języki publikacji: EN

Abstrakty

EN

Chaetognaths constitute a small marine phylum of approximately 120 species. Two classes of both 18S and 28S rRNA gene sequences have been evidenced in this phylum, even though significant intraindividual variation in the sequences of rRNA genes is unusual in animal genomes. These observations led to the hypothesis that this unusual genetic characteristic could play one or more physiological role(s). Using in situ hybridization on the frontal sections of the chaetognath Spadella cephaloptera, we found that the 18S Class I genes are expressed in the whole body, with a strong expression throughout the gut epithelium, whereas the expression of the 18S Class II genes is restricted to the oocytes. Our results could suggest that the paralog products of the 18S Class I genes are probably the “housekeeping” 18S rRNAs, whereas those of class II would only be essential in specific tissues. These results provide support for the idea that each type of 18S paralog is important for specific cellular functions and is under the control of selective factors.

Słowa kluczowe

EN

18S gene sequence; 28S rRNA gene sequence; phylum; gene expression; chaetognath; Spadella cephaloptera; in situ hybridization; duplication; expression pattern

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2007
• Tom: 12
• Numer: 4
• Opis fizyczny: p.573-583,fig.,ref.

Twórcy

autor

Barthelemy R -M

• Biodiversity and Environment, case 18, 3 Place V.Hugo, Universite de Provence, 13331 Marseille cedex 3, France

autor

Grino M.

autor

Pontarotti P.

autor

Casanova J.-P.

autor

Faure E.

Bibliografia

• 1. Casanova, J.-P. Chaetognatha. in: South Atlantic Zooplankton (Boltovskoy, D., Ed.), Backhuys Publishers, Leiden, 1999, 1353-1374.
• 2. Feigenbaum, D.L. and Maris, R.C. Feeding in chaetognatha. Oceanogr. Mar. Biol. Ann. Rev. 22 (1984) 343-392.
• 3. Matus, D.Q., Copley, R.R., Dunn, C.W., Hejnol, A., Eccleston, H., Halanych, K.M., Martindale, M.Q. and Telford, M.J. Broad taxon and gene sampling indicate that chaetognaths are protostomes. Curr. Biol. 16 (2006) R575-576.
• 4. Marletaz, F., Martin, E., Perez, Y., Papillon, D., Caubit, X., Lowe, C.J., Freeman, B., Fasano, L., Dossat, C., Wincker, P., Weissenbach, J. and Le Parco, Y. Chaetognath phylogenomics: a protostome with deuterostome-like development. Curr. Biol. 16 (2006) R577-R578.
• 5. Casanova, J.-P., Duvert, M. and Perez, Y. Phylogenetic interest of the chaetognath model. Mésogée 59 (2001) 27-31.
• 6. Jean, S., De Jong, L. and Moreau, X. Chaetognaths: a useful model for studying heat shock proteins. Effect of wound healing. J. Exp. Marine Biol. Ecol. 312 (2004) 319-332.
• 7. Takada, N, Goto, T. and Satoh, N. Expression pattern of the Brachyury gene in the arrow worm Paraspadella gotoi (chaetognatha). Genesis 32 (2002) 240-245.
• 8. Goto, T. and Yoshida, M. Growth and reproduction of the benthic arrowworm Paraspadella gotoi (Chateognatha) in laboratory culture. Invert. Reprod. Dev. 32 (1997) 201-207.
• 9. Prokopowich, C.D., Gregory, T.R. and Crease, T.J. The correlation between rDNA copy number and genome size in eukaryotes. Genome 46 (2003) 48-50.
• 10. Rooney, A.P. Mechanisms underlying the evolution and maintenance of functionally heterogeneous 18S rRNA genes in apicomplexans. Mol. Biol. Evol. 21 (2004) 1704-1711.
• 11. Ledee, D.R., Seal, D.V. and Byers, T.J. Confirmatory evidence from 18S rRNA gene analysis for in vivo development of propamidine resistance in a temporal series of Acanthamoeba isolates from a patient. Antimicrob. Agents Chemother. 42 (1998) 2144-2145.
• 12. Stothard, J.R., Frame, I.A., Carrasco, H.J. and Miles, M.A. Temperature gradient gel electrophoresis (TGGE) analysis of riboprints from Trypanosoma cruzi. Parasitology 117 (1998) 249-253.
• 13. Carranza, S, Baguna, J. and Riutort, M. Origin and evolution of paralogous rRNA gene clusters within the flatworm family Dugesiidae (Platyhelminthes, Tricladida). J. Mol. Evol. 49 (1999) 250-259.
• 14. Bonnaud, L., Saihi, A. and Boucher-Rodoni, R. Are 28SrDNA and 18SrDNA informative for cephalopod phylogeny? Bull. Mar. 71 (2003) 197-208.
• 15. Krieger, J. and Fuerst, P.A. Characterization of nuclear 18S rRNA gene sequence diversity and expression in an individual lake sturgeon (Acipenser fulvescens). J. Appl. Ichthyol. 20 (2004) 433-439.
• 16. Krieger, J., Hett, A.K., Fuerst, P.A., Birstein, V.J. and Ludwig, A. Unusual intraindividual variation of the nuclear 18S rRNA gene is widespread within the acipenseridae. J. Hered. 97 (2006) 218-225.
• 17. Papillon, D., Perez, Y., Caubit, X. and Le Parco, Y. Systematics of Chaetognatha under the light of molecular data, using duplicated ribosomal 18S DNA sequences. Mol. Phyl. Evol. 38 (2006) 621-634.
• 18. Telford, M.J. and Holland, P.W.H. Evolution of 28S ribosomal DNA in Chaetognaths: duplicate genes and molecular phylogeny. J. Mol. Evol. 44 (1997) 135-144.
• 19. Qari, S.H., Goldman, I.F., Pieniazek, N.J., Collins, W.E. and Lal, A.A. Blood and sporozoite stage-specific small subunit ribosomal RNA-encoding genes of the human malaria parasite Plasmodium vivax. Gene 150 (1994) 43-49.
• 20. Thompson, J., van Spaendonk, R.M., Choudhuri, R., Sinden, R.E., Janse, C.J. and Waters, A.P. Heterogeneous ribosome populations are present in Plasmodium berghei during development in its vector. Mol. Microbiol. 31 (1999) 253-360.
• 21. Grino, M and Zamora, A.J. An in situ hybridisation histochemistry technique allowing simultaneous visualization by the use of confocal microscopy of three cellular mRNA species in individual neurons. J. Histochem. Cytochem. 46 (1998) 753-759.
• 22. Gutell, R.R., Weibser, B., Woese, C.R. and Noller, H.F. Comparative anatomy of 16S-like ribosomal RNA. Prog. Nucleic. Acid. Res. Mol. Biol. 32 (1985) 155-216.
• 23. Shinn, G.L. Chaetognaths. in: Microscopic anatomy of invertebrates, Vol. 15, Hemichordates, Chaetognatha and the invertebrate chordates (Harrison, F.W. and Ruppert, E.E., Eds.), Wiley-Liss, New York, 1997, 103- 220.
• 24. Ghirardelli, E. Some aspects of the biology of the Chaetognaths. Adv. Mar. Biol. 6 (1968) 271-375.
• 25. Canipari, R., Pietrolucci, A. and Mangia, F. Increase of total protein synthesis during mouse oocyte growth. J. Reprod. Fertil. 57 (1979) 405- 413.
• 26. Mercereau-Puijalon, O., Barale, J.C. and Bischoff, E. Three multigene families in Plasmodium parasites: facts and questions. Int. J. Parasitol. 32 (2002) 1323-1344.
• 27. Komiya, H., Hasegawa, M. and Takemura, S. Differentiation of oocyte-type and somatic-type 5S ribosomal-RNAs in animals. J. Biochem. 100 (1986) 369-374.
• 28. Paillisson, A., Levasseur, A., Gouret, P., Callebaut, I., Bontoux, M., Pontarotti, P. and Monget, P. Bromodomain testis-specific protein is expressed in mouse oocyte and evolves faster than its ubiquitously expressed paralogs BRD2, -3, and -4. Genomics 89 (2007) 215-223.
• 29. Yang, J., Su, A.I. and Li, W.H. Gene expression evolves faster in narrowly than in broadly expressed mammalian genes. Mol. Biol. Evol. 22 (2005) 2113-2118.