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2008 | 64 | 06 |
Tytuł artykułu

Nowoczesne aspekty diagnostyki molekularnej brucelozy

Autorzy
Warianty tytułu
EN
New aspects of molecular diagnostics of brucellosis
Języki publikacji
PL
Abstrakty
EN
The diagnostics of brucellosis is primarily based on serological investigations. Since a serologically positive response can occur from antigenically cross-reactive bacteria, the isolation and identification of the pathogen from a potentially infected host remains the gold standard as a tool for the confirmation of infection. However, this process also has drawbacks. The tests are time consuming, complex and must be performed by highly skilled personnel. The zoonotic nature of most Brucella species is a potential hazard for laboratory personnel who must manipulate the infectious agent during testing. Finally, some of the characteristics are subjective and the results are not always definitive. Recently, progress in these aspects of diagnostics has been advanced through PCR-based technologies. PCR-based assays are simple to design and carry out, rapid, inexpensive to perform and are characterized by high diagnostic values: sensitivity and specificity. The following is a review of the major assays constituting excellent progress in the diagnostics of brucellosis.
Wydawca
-
Rocznik
Tom
64
Numer
06
Opis fizyczny
s.741-744,bibliogr.
Twórcy
autor
  • Panstwowy Instytut Weterynaryjny - Panstwowy Instytut Badawczy, Al.Partyzantow 57, 24-100 Pulawy
autor
Bibliografia
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  • 3. Bricker B. J., Ewalt D. R., Halling S. M.: Brucella "HOOF"-Prints: strain typing by multi-locus analysis of variable number tandem repeats (VNTRs). BMC Microbiol. 2003, 3, 15.
  • 4. Bricker B. J., Ewalt D. R., MacMillan A. P., Foster G., Brew S.: Molecular characterization of Brucella strains isolated from marine mammals. J. Clin. Microbiol. 2000, 38, 1258-1262.
  • 5. Bricker B. J., Halling S. M.: Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J. Clin. Microbiol. 1994, 32, 2660-2666.
  • 6. Cloeckaert A., Grayon M., Grepinet O., Boumedine K. S.: Classification of Brucella strains isolated from marine mammals by infrequent restriction sit-PCR and development of specific PCR identification tests. Microbes Infect. 2003, 5, 593-602.
  • 7. Cloeckaert A., Verger J. M., Grayon M., Grepinet O.: Restriction site polymorphism of the genes encoding the major 25 kDa and 26 kDa outer-membrane proteins of Brucella. Microbiology. 1995, 141, 2111-2121.
  • 8. Fekete A., Bantle J. A., Halling S. M., Sanborn M. R.: Preliminary development of a diagnostic test for Brucella using polymerase chain reaction. J. Appl. Bacteriol. 1990, 69, 216-227.
  • 9. Foster G., MacMillan A. P., Godfroid J., Howie F., Ross H. M., Cloeckaert A., Reid R. J., Brew S., Patterson I. A. P.: A review of Brucella sp. infection of sea mammals with particular emphasis on isolates from Scotland. Vet. Microbiol. 2002, 91, 563-580.
  • 10. Fox K. F., Fox A., Nagpal M., Steinberg P., Heroux K.: Identification of Brucella by ribosomal-spacer-region PCR and differentiation of Brucella canis from other Brucella spp. pathogenic for humans by carbohydrate profiles. J. Clin. Microbiol. 1998, 36, 3217-3222.
  • 11. Herman L., De Ridder H.: Identification of Brucella spp. by using the polymerase chain reaction. App. Environ. Microbiol. 1992, 54, 2099-2101.
  • 12. Leal-Klevezas D. S., Martinez-Vazquez I. O., Lopez-Merino A., Martinez-Soriano J. P.: Single-step PCR for detection of Brucella spp. from blood and milk of infected animals. J. Clin. Microbiol. 1995, 33, 3087-3090.
  • 13. Leyla G., Kadri G., Umran O.: Comparison of polymerase chain reaction and bacteriological culture for the diagnosis of sheep brucellosis using aborted fetus samples. Vet. Microbiol. 2003, 93, 53-61.
  • 14. Manterola L., Tejero-Garces A., Ficapal A., Shopayeva G., Blasco J. M., Marin C. M., Lopez-Goni I.: Evaluation of PCR test for the diagnosis of Brucella ovis infection in semen samples from rams. Vet. Microbiol. 2003, 92, 65-72.
  • 15. Matar G. M., Khneisser I. A., Abdelnoor A. M.: Rapid laboratory confirmation of human brucellosis by PCR. Analysis of a target sequence on the 31-kilodalton Brucella antigen DNA. J. Clin. Microbiol. 1996, 34, 477-478.
  • 16. Mercier E., Jumas-Bilak E., Allardet-Servent A., O'Callaghan D., Ramuz M.: Polymorphism in Brucella strains detected by studying distribution of two short repetitive DNA elements. J. Clin. Microbiol. 1996, 34, 1299-1302.
  • 17. Morata P., Queipo-Ortuno M. I., Reguera J. M., Garcia-Ordonez M. A., Pichardo C., de Dios Colmenero J.: Posttreatment Follow-up of brucellosis by PCR assay. J. Clin. Microbiol. 1999, 37, 4163-4166.
  • 18. Morata P., Queipo-Ortuno M. I., Reguera J. M., Garcia-Ordonez M. A., Cardenas A., Colmenero J. D.: Development and evaluation of a PCR-enzyme-linked immunosorbent assay for diagnosis of human brucellosis. J. Clin. Microbiol. 2003, 41, 144-148.
  • 19. Navarro E., Casao M. A., Solera J.: Diagnosis of human brucellosis using PCR. Exper. Rev. Mol. Diagn. 2004, 4, 115-123.
  • 20. Newby D. T., Hadfield T. L., Roberto F. F.: Real-time PCR detection of Brucella abortus: a comparative study of SYBR green I, 5 - exonuclease, and hybridization Probe assays. Appl. Environ. Microbiol. 2003, 69, 4753-4759.
  • 21. Queipo-Ortuno M. I., Morata P., Ocon P., Manchado P., de Dios Colmonero J.: Rapid diagnosis of human brucellosis by peripheral - blood PCR assay. J. Clin. Microbiol. 1997, 35, 2927-2930.
  • 22. Redkar R., Rose S., Bricker B., DelVecchio V.: Real-time detection of Brucella abortus, Brucella melitensis and Brucella suis. Mol. Cell Probes 2001, 15, 43-52.
  • 23. Richtzenhain L. J., Cortez A., Heinemann M. B., Soares R. M., Sakamoto S. M., Vasconcellos S. A., Higa Z. M. M., Scarcelli E., Genovez M. E.: A multiplex PCR for detection of Brucella spp. and Leptospira spp. DNA from aborted bovine fetuses. Vet. Microbiol. 2002, 87, 139-147.
  • 24. Rijpens N. P., Jannes G., van Asbroeck M., Rossau R., Herman L. M. F.: Direct detection of Brucella spp. in raw milk by PCR and reverse hybridization with 16S-23S rRNA spacer probes. Appl. Environ. Microbiol. 1996, 62, 1683-1688.
  • 25. Romero C., Gamazo C., Pardo M., Lopez-Goni I.: Specific detection of Brucella DNA by PCR. J. Clin. Microbiol. 1995, 33, 615-617.
  • 26. Romero C., Lopez-Goni I.: Improved method for purification of bacterial DNA from bovine milk for detection of Brucella spp. by PCR. Appl. Environ. Microbiol. 1999, 65, 3735-3737.
  • 27. Romero C., Pardo M., Grillo M. J., Diaz R., Blasco J. M., Lopez-Goni I.: Evaluation of PCR and indirect enzyme-linked immunosorbent assay on milk samples for diagnosis of brucellosis in dairy cattle. J. Clin. Microbiol. 1995, 33, 3198-3202.
  • 28. Tcherneva E., Rijpens N., Naydensky C., Herman L.: Differentiation of Brucella species by random amplified polymorphic DNA analysis. J. Appl. Microbiol. 2000, 88, 69-80.
  • 29. Whatmore A. M., Shankster S. J., Perrett L. L., Murphy T. J., Brew S. D., Thirlwall R .E., Cutler S. J., MacMillan A. P.: Identification and characterization of variable-number tandem-repeat markers for typing of Brucella spp. J. Clin. Microbiol. 2006, 44, 1982-1993.
  • 30. Zerva L., Bourantas K., Mitka S., Kansouzidou A., Legakis N. J.: Serum is the preffered clinical specimen for diagnosis of human brucellosis by PCR. J. Clin. Microbiol. 2001, 39, 1661-1664.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-article-913830a5-7400-4b96-839f-dd0325f70930
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