Nucleotide sequence polymorphisms in the promoter region of bovine growth hormone receptor gene [GHR] have no effect on its expression level in liver

• Autorzy: Maj A , Zwierzchowski L.

Warianty tytułu

PL

Polimorfizm sekwencji nukleotydowej w rejonie promotorowym genu receptora hormonu wzrostu [GHR] bydla nie wplywa na ekspresje tego genu w watrobie

• Języki publikacji: EN

Abstrakty

EN

The gene coding for bovine GHR consists of nine protein-coding exons and untranslated, alternative exons 1A, 1B, and 1C in its 5’-region. Distinct promoters regulate transcription from each of the alternative exons. The P1 promoter which drives growth hormone receptor expression in the liver is associated with exon 1A. Earlier the nucleotide sequence polymorphisms have been identified in the bovine GHR gene promoter region, including several single nucleotide polymorphisms (SNPs) and one TG repeat (microsatellite) of variable length. Using computer-aided analysis in TESS programme it has also been shown that the A/G transition at position -154 (RFLP-NsiI) and the C/T transition at position -1104 (Fnu4HI), both located upstream the exon 1A, co-localized with putative transcription factor-binding sites. In light of this the authors decided to study possible effects of these polymorphisms on GHR gene expression in cattle of different GHR genotypes, using Real-time PCR. Interestingly, no difference was found in GHR mRNA accumulation in liver between young Black-and-White (BW) bulls carrying (+/+), (+/-) or (-/-) genotypes at RFLP-NsiI site, (+/-) or (+/+) genotypes at RFLP-Fnu4HI site, and TG17/17 or TG21/21 alleles at TGn microsatellite, located within the P1 promoter of the bovine GHR gene.

PL

Gen kodujący receptor hormonu wzrostu bydła zbudowany jest z dziewięciu eksonów kodujących białko i trzech eksonów (1A, 1B, i 1C) nie podlegających translacji, których transkrypcja prowadzi do powstania trzech typów mRNA, różniących się długością rejonu 5’-UTR. Ekspresją transkryptu występującego w wątrobie kieruje promotor P1, położony „powyżej” eksonu 1A. W rejonie promotorowym genu GHR bydła występuje kilka polimorfizmów typu SNP, a także polimorficzna sekwencja mikrosatelitarna TGn. W prezentowanej tu pracy, w wyniku analizy komputerowej w programie TESS stwierdzono, że niektóre z tych mutacji leżą w miejscu wiązania czynników transkrypcyjnych. Postanowiono zatem poszukiwać zależności między polimorfizmem sekwencji nukleotydów w rejonie promotorowym genu GHR bydła a ekspresją tego genu w wątrobie. Używano techniki Real-time, a jako matrycę stosowano RNA, wyizolowany z wątroby młodych buhajów cb o różnych genotypach GHR. Nie wykazano różnic w poziomie mRNA GHR w wątrobie między buhajami o genotypach (+/+), (+/-) i (-/-) w odniesieniu do tranzycji A/G w pozycji ‑154 (RFLP-NsiI), o genotypach (+/-) i (+/+) dla tranzycji C/T w pozycji ‑1104 (RFLP-Fnu4HI) ani o genotypach TG17/17 i TG21/21 w odniesieniu do sekwencji mikrosatelitarnej w promotorze P1.

Słowa kluczowe

EN

growth hormone receptor gene; liver; gene expression; animal breeding; nucleotide sequence; cattle; genotype; polymorphism; growth hormone

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2008
• Tom: 26
• Numer: 1
• Opis fizyczny: p.5-15,fig.,ref.

Twórcy

autor

Maj A

• Polish Academy of Sciences, Institute of Genetics and Animal Breeding, Jastrzebiec, 05-552 Wolka Kosowska, Poland

autor

Zwierzchowski L.

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