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2004 | 09 | 1 |
Tytuł artykułu

A high frequency of apoptosis was found in cultures of lymphocytes isolated from the venous blood of children born with a low birth weight

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Children born with a low birth weight (below 2500g) exhibit a slower rate of development, and a greater tendency towards morbidity and mortality, together with a deficit of weight and height. One reason could be an increase in the level of cell elimination by apoptosis. The aim of this study was to evaluate and compare the incidence of apoptotic and necrotic (dead) cells in cultures of peripheral blood lymphocytes obtained from children born with a low birth weight and from children with a normal birth weight. Peripheral blood lymphocytes were obtained by venipuncture (10 ml) and isolated using the density gradient centrifugation method. The lymphocytes were cultured for 48 h in a culture medium containing low concentrations of fetal calf serum. A comparison study was performed between low birth weight children and normal birth weight children and the susceptibility of their lymphocytes to apoptosis and to necrosis in serum-deficient feeding culture conditions. The amount of apoptotic cells and the percentage of dead cells were significantly higher in cultures of lymphocytes obtained from low birth weight children than in cultures from normal birth weight children. The two estimated parameters inversely correlated with the concentration of fetal calf serum in the culture medium. Pulsed field gel electrophoresis showed increased DNA degradation patterns in the cultures of lymphocytes obtained from low birth weight children. Our results should be perceived as an indication that, under worse feeding conditions, the elimination of cells by apoptosis and by necrosis is significantly higher for lymphocytes of low birth weight children than for those of normal birth weight children. The enhanced elimination of lymphocytes is related to a greater susceptibility to infections, especially of the respiratory tract, as established in the retrospective analysis of the anamneses of the examined group of low birth weight children.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
09
Numer
1
Opis fizyczny
p.135-143,fig.,ref.
Twórcy
autor
  • Wroclaw Medical University, Wroclaw, Poland
autor
autor
autor
Bibliografia
  • 1. Darlow, B.A., Horwood, L.J. and Mogridge, N. Very low birth weight and asthma by age seven years in a national cohort. Pediat. Pulmonol. 30 (2000) 291-296.
  • 2. Botero, D. and Lifshitz, F. Interautrine growth retardation and long-term effects on growth. Curr. Opin. Pediat. 11 (1999) 340-347.
  • 3. Dennison, E., Fall, C., Cooper, C. and Barker, D. Prenatal factors influencing long-term outcome. Horm. Res. 48 suppl 1 (1997) 72-74.
  • 4. Holmes, R.P. and Soothill, P.W. Intrauterine growth retardation. Curr. Opin. Obstet. Gynecol. 8 (1996) 148-154.
  • 5. Barker, D.J. Outcome of low birthweight. Horm. Res. 42 (1994) 223-230.
  • 6. Gluckman, P.D., Cutfield, W., Harding, J.E., Milner, D., Woodhall, S., Gallaher, B., Bauer, M. and Breier BH. Metabolic consequences of intrauterine growth retardation. Acta Paediat. 417 suppl. (1996) 3-6.
  • 7. Engelbregt, M.J.T., Houdijk, M.E., Popp-Snijders, C. and Delemarrevan de Waal, H. The effects of intra-uterine growth retardation and postnatal undernutrition on onset of puberty in male and female rats. Pediat. Res. 48 (2000) 803-807.
  • 8. Albertsson-Wikland, K., Boguszewski, M. and Karlberg, J. Children born small-for-gestional age: postnatal growth and hormonal status. Horm. Res. 49 suppl 2 (1998) 7-13.
  • 9. Gluckman, P.D. and Harding, J.E. The physiology and pathophysiology of intrauterine growth retardation. Horm. Res. 48, suppl 1 (1997) 11-16.
  • 10. Albertsson-Wikland, K. and Karlberg, J. Natural growth in children born small for gestational age with and without catch-up growth. Acta Paediat. 399 suppl. (1994) 64-70.
  • 11. Frank, G.F., Cheung, P.-T., Horn, J.A., Alfaro, M.P., Smith, E.P. and Chernausek, S.D. Predicting the growth response to growth hormone in patients with intrauterine growth retardation. Clin. Endocrinol. 44 (1996) 679-685.
  • 12. Wollmann, H.A. Intrauterine growth restriction: definition and etiology. Horm. Res. 49 suppl 2 (1998) 1-6.
  • 13. Hofman, F.M., Kanesberg, B. and Smith, D. Stability of T- and B-cell numbers in human peripheral blood. Amer. J. Pathol. 77 (1982) 710-712.
  • 14. Duke, R.C. and Cohen, J.J. Morphological and biochemical assays of apoptosis. Curr. Prot. Immunol. 17 suppl 3 (1992) 1-16.
  • 15. Gąsiorowski, K., Brokos, B., Kulma, A., Ogorzałek, A. and Skórkowska, K. A comparison of the methods applied to detect apoptosis in genotoxically-damaged lymphocytes cultured in the presence of four antimutagens. Cell. Mol. Biol. Lett. 6 (2001) 141-159.
  • 16. Gąsiorowski, K., Brokos, B., Kulma, A., Ogorzałek, A. and Skórkowska, K. Impact of four antimutagens on apoptosis in genotoxically damaged lymphocytes in vitro. Cell. Mol. Biol. Lett. 6 (2001) 649-675.
  • 17. Dengler, W.A., Schulte, J., Berger, D.P., Mertelsmann, R. and Fiebig, H.H. Development of a propidium iodide fluorescence assay for proliferation and cytoxicity assays. Anti-Cancer Drug 6 (1995) 522-532.
  • 18. Markiewicz, E., Wilczynski, G., Filipski, J. and Szopa, J. Modification of the apoptotic-like effects of MBP protein overexpression in E.coli by fusion with 14-3-3 derived polypeptides. Cell Death Differ. 4 (1997) 272-275.
  • 19. Campbell, M.J. and Machin, D. Statistical inference. Correlation and regression. In: Medical Statistics. 2nd ed (Campbell, M.J. and Machin, D. Eds) John Willey and Sons Ltd., Chichester (1994) 69-104.
  • 20. Fernandez-Botran, R. and Vetvicka, V. Proliferation assays. In: Methods in Cellular Immunology (Fernandez-Botran, R., Ed.) CRC Press, Boca Raton, New York, London, Tokyo (1995) 47-51.
Typ dokumentu
Bibliografia
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Identyfikator YADDA
bwmeta1.element.agro-article-90846213-b388-47fa-bf5e-dcce8ed34fa4
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