Cloning of the DNA fragments reflecting the open reading frame I and II of the I-18 C gene of Chironomus tentans. VI. The DNA sequencing of the cloned inserts

• Autorzy: Borowicz B P
• Języki publikacji: EN

Abstrakty

EN

The technology used for the cloning of the open reading frame (ORF) I and II reflecting DNA fragments in regard to the DNA sequencing of the cloned inserts, is presented. The object of the DNA sequencing of the cloned inserts, was to finally confirm and prove the sequence of the inserts as the sequence of the ORF I and II reflecting DNA fragments of the I-18 C gene of Chironomus tentans.

PL

Przedstawiono technologię, zastosowaną do klonowania fragmentów DNA odzwierciedlających otwartą ramę odczytu I i II genu I-18 C Chironomus tentans w zakresie sekwencjonowania sklonowanych wstawek. Celem sekwencjonowania DNA sklonowanych wstawek było ostateczne potwierdzenie i udowodnienie, że sekwencje wklonowanych wstawek były sekwencjami fragmentów DNA odzwierciedlających otwartą ramę odczytu I i II genu I-18 C Chironomus tentans.

Słowa kluczowe

EN

DNA sequence; pET-3a vector; promoter system; cloning; I-18 C gene; T7 RNA polymerase; Chironomus tentans; polymerase chain reaction; expression; DNA fragment

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 1999
• Tom: 39
• Numer: 1
• Opis fizyczny: p.63-67

Twórcy

autor

Borowicz B P

• Institute of Plant Protection, Miczurina 20, 60-318 Poznan, Poland

Bibliografia

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