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1999 | 39 | 1 |
Tytuł artykułu

Cloning of the DNA fragments reflecting the open reading frame I and II of the I-18 C gene of Chironomus tentans. VI. The DNA sequencing of the cloned inserts

Autorzy
Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The technology used for the cloning of the open reading frame (ORF) I and II reflecting DNA fragments in regard to the DNA sequencing of the cloned inserts, is presented. The object of the DNA sequencing of the cloned inserts, was to finally confirm and prove the sequence of the inserts as the sequence of the ORF I and II reflecting DNA fragments of the I-18 C gene of Chironomus tentans.
PL
Przedstawiono technologię, zastosowaną do klonowania fragmentów DNA odzwierciedlających otwartą ramę odczytu I i II genu I-18 C Chironomus tentans w zakresie sekwencjonowania sklonowanych wstawek. Celem sekwencjonowania DNA sklonowanych wstawek było ostateczne potwierdzenie i udowodnienie, że sekwencje wklonowanych wstawek były sekwencjami fragmentów DNA odzwierciedlających otwartą ramę odczytu I i II genu I-18 C Chironomus tentans.
Wydawca
-
Rocznik
Tom
39
Numer
1
Opis fizyczny
p.63-67
Twórcy
autor
  • Institute of Plant Protection, Miczurina 20, 60-318 Poznan, Poland
Bibliografia
  • 1. Amrein K., Lutz B., Hertner T., Lezzi M., Dorsch-Haesler K. 1986. Complete DNA sequence of the ecdysteronc-controlled gene I-18 C of Chironomus tentans. Nucl. Acids Res., 14: 7807-7808.
  • 2. Borowicz B.P. 1995. Isolation of the DNA fragment reflecting the Open Reading Frame I of the I-18 C gene of Chironomus tentans by the Polymerase Chain Reaction. I. The technology for the preparation of the samples (in English). Prace Nauk. Inst. Ochr. Roślin 36 (1/2): 69-76.
  • 3. Borowicz B.P. 1995. Isolation of the DNA fragment reflecting the Open Reading Frame I of the I-18 C gene of Chironomus tentans by the Polymerase Chain Reaction. II. The technology required to obtain the DNA fragment (in English). Prace Nauk. Inst. Ochr. Roślin 36 (1/2): 77-82.
  • 4. Borowicz B.P. 1995. Isolation of the DNA fragment reflecting the Open Reading Frame I of the I-18 C gene of Chironomus tentans by the Polymerase Chain Reaction. III. The effect of the applied technologies (in English). Prace Nauk. Inst. Ochr. Roślin 36 (1/2): 83-93.
  • 5. Borowicz B.P. 1996. Isolation of the DNA fragment reflecting the Open Reading Frame II of the I-18 C gene of Chironomus tentans by the Polymerase Chain Reaction. I. The technology for the preparation of the primers. J. Plant Protection Res., 37 (1/2): 138-144.
  • 6. Borowicz B.P. 1996. Isolation of the DNA fragment reflecting the Open Reading Frame II of the I-18 C gene of Chironomus tentans by the Polymerase Chain Reaction. II. The technology for the preparation of the template. J. Plant Protection Res., 37 (1/2): 145-148.
  • 7. Borowicz B.P. 1996. lsolation of the DNA fragment reflecting the Open Reading Frame II of the I-18 C gene of Chironomus tentans by the Polymerase Chain Reaction. III. The DNA amplification technology. J. Plant Protection Res., 37 (1/2): 149-155.
  • 8. Borowicz B.P. 1996. Isolation of the DNA fragment reflecting the Open Reading Frame II of the I-18 C gene of Chironomus tentans by the Polymerase Chain Reaction. IV. The product of the applied technologies. J. Plant Protection Res., 37 (1/2): 156-164.
  • 9. Borowicz B. P. 1996. Isolation of the DNA fragment reflecting the Open Reading Frame II of the I-18 C gene of Chironomus tentans by the Polymerase Chain Reaction. V. Different mechanisms of regulation regarding the Open Reading Frames. J. Plant Protection Res., 37 (1/2): 165-171.
  • 10. Del Sal G.M., Manfioletti G., Schneider C. 1988. A one-tube plasmid DNA mini-preparation suitable for sequencing. Nucl. Acids Res., 16: 9878 pp.
  • 11. Hindley J. 1983. DNA scquencing. Elsevier Science Publishers.
  • 12. Lezzi M., Lutz B., Dorsch-Haesler K. 1989 b. The ecdysterone-controlled chromosome region I-18 C in C. tentans: from cytology to molecular biology. Acta Biol. Debr. Oecol. Hung., 2: 129-139.
  • 13. Sambrook J., Fritsch E.F., Maniatis T. 1989. Molecular Cloning. A laboratory manual. Second Edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, Books: 1-3.
  • 14. Sambrook J., Fritsch E.F., Maniatis T. 1989. Plasmid Vectors (chapter 1) in Molecular Cloning. A laboratory manual. Second Edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, Book 1: 1.2-1.110.
  • 15. Sambrook J., Fritsch E.F., Maniatis T. 1989. DNA Sequcncing (chapter 13) in Molecular Cloning. A laboratory manual. Second Edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, Book 2: 13.1-13.104.
  • 16. Sambrook J., Fritsch E.F., Maniatis T. 1989. Expression of Cloned Genes in Escherichia coli (chapter 17) in Molecular Cloning. A laboratory manual. Second Edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, Book 3: 17.2-17.44.
  • 17. Studier F.W., Rosenberg A.H., Dunn J.J., Dubendorff J.W. 1990. Use of T7 RNA Polymerase to direct expression of cloned genes. Methods in Enzymology, Goeddel D.V. (ed.), 1985: 60-89.
  • 18. Winnacker E.-L. 1987. From Genes to Clones. Introduction to Gene Technology (in English). VCH Verlags-gesellschaft mbH, D-6940 Weinheim, Germany: 1-634.
Typ dokumentu
Bibliografia
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Identyfikator YADDA
bwmeta1.element.agro-article-902c8a70-164f-4703-9b7e-08c1e1afa780
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