Immunocytochemical analysis of the tissue location of cytokines [IL-2 and IL-12] in neuroendocrine lung cancer

• Autorzy: Kasprzak A , Przewozna M. , Zabel M. , Pankowski J. , Surdyk-Zasada J.
• Języki publikacji: EN

Abstrakty

EN

The study aimed at immunocytochemical evaluation of the cellular expression of two cytokines, IL-2 and IL-12 in lung carcinoids (n = 10), following the earlier demonstration of two markers of endocrine tumours (chromogranin A and NSE — neuron-specific enolase). In the immunocytochemical studies the classical avidin-biotinylated peroxidase (ABC) technique was used. Results of the tests were semiquantitatively appraised employing the IRS scale. In 9/10 cases intense reaction (score: 6–12 points) was noted for both lung neuroendocrine markers. In all cases of carcinoma co-expression of IL-2 and IL-12 was demonstrated in cells of the tumours. The cytokines showed a cytoplasmic localisation of mean (score: 3–4 points) or high (score: 6–12 points) intensity of reaction. Our studies point to a possible role of the two cytokines in the proliferation of lung neuroendocrine carcinomas but more detailed analysis is required on a broader material.

Słowa kluczowe

EN

immunocytochemical analysis; tissue; cytokine; interleukin 12; interleukin 2; neuroendocrine; lung cancer; carcinoid

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2003
• Tom: 62
• Numer: 3
• Opis fizyczny: p.301-303,fig.,ref.

Twórcy

autor

Kasprzak A

• University of Medical Sciences, Swiecickiego 6, 60-781 Poznan, Poland

autor

Przewozna M.

autor

Zabel M.

autor

Pankowski J.

autor

Surdyk-Zasada J.

Bibliografia

• 1. Hsu S-M, Raine L, Fanger H (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques. J Histochem Cytochem, 29: 577–580
• 2. Lissoni P, Rovelli F, Giani L, Fumagalli L, Meregalli S, Comi G, Merlini D (1996) Correlation between IL-12 and IL-2 blood levels in the metastatic neoplastic disease: a possible inhibitory feedback system regulating their secretion. J Biol Regul Homeost Agents, 10: 92–94.
• 3. Remmele W, Stegner HE (1987) Vorschlag zur einheitlichen Definition eines immunoreactiven Score (IRS) fur den Immunohistochemichen Ostrogenrezeptor — Nachweis (ER-ICA) im Mammakarzinomgewebe. Pathologe, 8: 138–140.
• 4. Reichert TE, Watkins S, Stanson J, Johnson JT, Whiteside TL (1998) Endogenous IL-2 in cancer cells: a marker of cellular proliferation. J Histochem Cytochem, 46: 603–612.
• 5. Rydzewska-Rosołowska AE, Kasacka I, Sulewska A, Rudy A, Chyczewski L (2001) Pulmonary neuroendocrine cells in physiology and pathology. Folia Histochem Cytobiol, 39 (Suppl 2): 58–63.
• 6. Sheppard MN (2000) Specific markers for pulmonary tumours. Histopathology, 36: 273–276.
• 7. Yoshimura C, Nomura S, Kanazawa S, Kuwana M, Yamaguchi K, Fukuhura S (2002) Comparison of interleukin-12 with lung cancer and malignant lymphoma undergoing autologous peripheral blood stem cell transplantation. J Cancer Res Clin Oncol, 128: 29–36.