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2008 | 64 | 05 |
Tytuł artykułu

Identification of amplified fragment length polymorphism markers associated with freezability of boar semen - a preliminary study

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
In this study the amplified fragment length polymorphism (AFLP) technique, based on the analysis of genomic restriction fragments by polymerase chain reaction (PCR) amplifications, was utilized to identify genomic markers associated with differences in the freezability of boar semen. Collected from seven Polish Large White boars, semen was cooled in a lactose-lipoprotein fractions-glycerol extender (lac-LPFo-G), packaged into aluminum tubes and frozen using a programmable computer freezer. The DNA, isolated from each boar, was screened for AFLP markers using different primer pair combinations for selective amplifications. Sperm samples prior to and after freezing-thawing were analyzed for motility, mitochondrial function (Rhodamine 123), plasma membrane integrity (SYBR-14 positive) and DNA integrity using the single cell gel electrophoresis (neutral Comet assay). The authors’ preliminary findings have indicated that there were consistent inter-boar variations in terms of post-thaw sperm characteristics. Distinct differences in AFLP DNA patterns in each boar were detected using different primer combinations. Furthermore, amplified DNA fragments, with similar base pairs, were detected only in the DNA profile of boars with good semen freezability. The AFLP technique can be used to select reproductive boars that produce semen with good quality characteristics for freezing-thawing procedure.
Wydawca
-
Rocznik
Tom
64
Numer
05
Opis fizyczny
p.646-649,fig.,ref.
Twórcy
autor
  • Warmia and Mazury University, 10-718 Olsztyn, Poland
autor
autor
Bibliografia
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  • 2. Evenson D. P., Thompson L., Test L.: Flow cytometric evaluation of boar semen by the sperm chromatin structure assay as related to cryopreservation and fertility. Theriogenology 1994, 41, 637-651.
  • 3. Fraser L., Lecewicz M., Strze¿ek J.: Fluorometric assessments of viability and mitochondrial status of boar spermatozoa following liquid storage. Pol. J. Vet. Sci. 2002, 5, 85-92.
  • 4. Fraser L., Strzeżek J.: Effects of freezing-thawing on DNA integrity of boar spermatozoa assessed by the neutral comet assay. Reprod. Dom. Anim. 2005, 40, 530-536.
  • 5. Garner D. L., Johnson L. A.: Viability assessment of mammalian sperm using SYBR-14 and propidium iodide. Biol. Reprod. 1995, 53, 276-2284.
  • 6. Gil M. A., Roca J., Cremades T., Hernández M., Vázquez J. M., Rodriguez-Martínez H., Martinez E. A.: Does multivariate analysis of post-thaw sperm characteristics accurately estimate in vitro fertility of boar individual ejaculates? Theriogenology 2005, 64, 305-316.
  • 7. Holt W. V., Medrano A., Thurston L. M., Watson P. F.: The significance of cooling rates and animal variability for boar sperm cryopreservation: insights from the cryomicroscope. Theriogenology 2005, 63, 370-382.
  • 8. Johnson L. A., Weitze K. F., Fiser P., Maxwell W. M. C.: Storage of boar semen. Anim. Reprod. Sci. 2000, 62, 143-172.
  • 9. Medrano A., Watson P. F., Holt W. V.: Importance of cooling rate and animal variability for boar sperm cryopreservation: insights from the cryomicroscope. Reproduction 2002, 123, 315-322.
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  • 12. Strzeżek J.: Secretory activity of boar seminal vesicle glands. Reprod. Biol. 2002, 3, 243-266.
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  • 14. Strzeżek J., Lecewicz M., Dziekońska A., Fraser L.: A simple method of extraction of lipoprotein fractions from avian egg yolk – protective effect on cooled boar semen. Theriogenology 2005, 6, 496-497.
  • 15. Thurston L. M., Siggins K., Mileham A. J., Watson P. F., Holt W. V.: Identification of amplified restriction fragment polymorphism markers linked to genes controlling boar sperm viability following cryopreservation. Biol. Reprod. 2002, 66, 545-554.
  • 16. Thurston L. M., Watson P. F., Holt W. V.: Semen cryopreservation: a genetic explnantion for species and individual variation? CryoLetters 2002, 23, 255-262.
  • 17. Vos P., Hogers R., Bleeker M., Rijans M., Van de Lee T., Hornes M., Frijters A., Pot J., Peleman J., Kuiper M., Zabeau M.: AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res.1995, 23, 4407-4414.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-article-8f8457a3-007a-4c88-b5e4-579e53240a9b
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