Use of inter-simple sequence repeat markers to develop strain-specific SCAR markers for Flammulina velutipes

• Autorzy: Su H , Wang L , Liu L , Chi X , Zheng Y
• Języki publikacji: EN

Abstrakty

EN

In this paper, we report for the first time on authentication of Flammulina velutipes cultivare by using strain-specific sequence-characterized amplified region (SCAR) markers developed from inter-simple sequence repeat (ISSR) markers. The genomic DNA polymorphism was analyzed by the ISSR technique in 7 strains of F. velutipes presently cultivated in China on a commercial scale. Eight primers selected from 20 ISSR primers amplified 104 clear and stable bands, of which 81 bands were polymorphic. Among the selected primers, primer ISSR9 can distinguish strain No. 12 from the other 6 strains by amplifying a unique and reproducible band of approximately 750 bp. According to the sequence of the strain-specific fragment, a pair of SCAR primers was designed to diagnose strain No. 12 on the molecular level. The validity of the SCAR marker was confirmed by using DNA samples from another 12 strains of F. velutipes obtained from different parts of China. Our data provided the foundation for a precise and rapid PCR-based strain-diagnostic system for F. Velutipes.

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2008
• Tom: 49
• Numer: 3
• Opis fizyczny: p.233-235,fig.,ref.

Twórcy

autor

Su H

• College of Life Sciences, Ludong University, 186 Hongqizhong Rd., Yantai 264025, Yantai, China

autor

Wang L

• College of Life Sciences, Ludong University, 186 Hongqizhong Rd., Yantai 264025, Yantai, China

autor

Liu L

• College of Life Sciences, Ludong University, 186 Hongqizhong Rd., Yantai 264025, Yantai, China

autor

Chi X

• College of Life Sciences, Ludong University, 186 Hongqizhong Rd., Yantai 264025, Yantai, China

autor

Zheng Y

• College of Life Sciences, Ludong University, 186 Hongqizhong Rd., Yantai 264025, Yantai, China

Bibliografia

• Albani MC, Battey NH, Wilkinson MJ, 2003. The development of ISSR-derived SCAR markers around the SEASONAL FLOWERING LOCUS (SFL ) in Fragaria vesca. Theor Appl Genet 109: 571-579.
• Bornet B, Branchard M, 2001. Nonanchored inter simple sequence repeat (ISSR) markers: reproducible and specific tools for genome fingerprinting. Plant Mol Biol Rep 19: 209-215.
• Dnyaneshwar W, Preeti C, Kalpana J, Bhushan P, 2006. Development and application of RAPD-SCAR marker for identification of Phyllanthus emblica LINN. Biol Pharm Bull 29: 2313-2316.
• Esselman EJ, Li JQ, Crawford DJ, Winduss JL, Wolfe AD, 1999. Clonal diversity in the rare Calamagrostis porteri ssp. insperata (Poaceae): comparative results for allozymes and random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. Mol Ecol 8: 443-451.
• Fang DQ, Roose ML, 1997. Identification of closely related citrus cultivars with inter-simple sequence repeat markers. Theor Appl Genet 95: 408-417.
• Fernandez S, Costa AC, Katsuyama ÂM, Madeira AMBN, Gruber A, 2003. A survey of the inter- and intraspecific RAPD markers of Eimeria spp. of the domestic fowl and the development of reliable diagnostic tools. Parasitol Res 89: 437-445.
• He L, Wang SB, Miao XX, Wu H, Huang YP, 2007. Identification of necrophagous fly species using ISSR and SCAR markers. Forensic Sci Int 168: 148-153.
• Leifa F, Pandey A, Soccol CR, 2001. Production of Flammulina velutipes on coffee husk and coffee spent-ground. Braz Arch Biol Techn 44: 205-212.
• Nagaoka T, Ogihara Y, 1997. Applicability of inter- simple sequence repeat polymorphism in wheat for use as DNA markers in comparison to RFLP and RAPD markers. Theor Appl Genet 94: 597-602.
• Noël T, Labarére J, 1987. Isolation of DNA from Agrocybe aegerita for the construction of a genomic library in E. coli. Mushroom Sci 12: 187-201.
• Paran I, Michelmore RW, 1993. Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor Appl Genet 85: 985-993.
• Qin LH, Tan Q, Chen MJ, Pan YJ, 2006. Use of intersimple sequence repeats markers to develop strain-specific SCAR markers for Lentinula edodes. FEMS Microbiol Lett 257: 112-116.
• Rakoczy-Trojanowska M, Bolibok H, 2004. Characteristics and a comparison of three classes of microsatellite-based markers and their application in plants. Cell Mol Biol Lett 9: 221-238.
• Tsumura Y, Ohba K, Strsuss SH, 1996. Diversity and inheritance of inter-simple sequence repeat polymorphisms in Douglas-fir (Pseudotsuga menziesii) and sugi (Cryptomeria japonica). Theor Appl Genet 92: 40-45.
• Virk PS, Zhu J, Newbury HJ, 2000. Effectiveness of different classes of molecular marker for classifying and revealing variation in rice germplasm. Euphytica 112: 275-284.
• Zietkiewicz E, Rafalski A, Labuda D, 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20: 176-183.