PL
Badano oddziaływanie kwasu propionowego, adypinowego i mlekowego oraz ich soli na wzrost toksynotwórczego szczepu Penicillium expansum i dynamikę wytwarzania przez niego patuliny. Poziom toksyny oznaczano metodą chromatografii cienkowarstwowej i wysokociśnieniowej chromatografii cieczowej. Ustalono najniższe stężenia badanych środków konserwujących całkowicie hamujących rozwój mikrogrzyba i biosyntezę patuliny.
EN
Effect of propionic acid, adipic acid, lactic acid, and salts of these acids, on the growth of Penicillium expansum and the dynamics of patulin formation by this strain, was investigated. Toxin level was assayed by TLC and HPLC methods. Minimal concentration of preservatives inhibiting completely microfungus growth and patulin biosynthesis, was determined. Propionic acid and adipic acid at the concentration of 0.05 mol/dm3, and sodium propionate at the concentration of 0.2 mol/dm3 were found to inhibit completely the gain in weight of Penicillium expansum mycelium and the toxin formation. Calcium lactate at the concentration upwards of 0.25 mol/dm3, was noted to counteract the formation of patulin, and - at 0.5 mol/dm3 - to inhibit completely the fungus growth. Lactic acid was least active of all preservatives under test. At the concentration up. to 0.5 mol/dm3, this agent inhibited the strain growth only by 33%, and patulin biosynthesis - by 85%.