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1997 | 44 | 4 |
Tytuł artykułu

Continuous assay for acid phosphatase using 1-naphthyl phosphate as a substrate

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The described continuous acid phosphatase assay is based on kinetics of the release of 1-naphthol in the course of the enzyme-catalyzed hydrolysis of 1-naphthyl phosphate, measured at 320 nm in aqueous solution and at 322 nm in sodium-bis(2-ethylhexyl)sulfosuccinate isooctane-water reverse micelles in a broad pH range (1.0-8.2). The method allows precise determination of the initial rate of the reaction and therefore may be used in the steady-state and pre-steady-state studies on the phosphatase-catalyzed reaction. The kinetic parameters (Km and kcat) for human prostatic acid phosphatase in aqueous solution and in reverse micelles, at pH 3.8, 4.5 and 5.7, by the proposed 1-naphthyl phosphate assay have been determined.
Wydawca
-
Rocznik
Tom
44
Numer
4
Opis fizyczny
p.853-859,fig.
Twórcy
  • Collegium Medicum, Jagiellonian University, M.Kopernika 7, 31-034 Cracow, Poland
Bibliografia
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Typ dokumentu
Bibliografia
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Identyfikator YADDA
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