Differentiated Paju cells have increased resistance to toxic effects of potassium ionophores

• Autorzy: Teplova V. , Jaaskelainen E. , Salkinoja-Salonen M. , Saris N.E.L. , Serlachius M. , Li F.Y. , Andersson L.C.
• Języki publikacji: EN

Abstrakty

EN

In this study we have investigated the impact of differentiation of neuronal cells on their sensitivity to microbial toxins. We used the human neural crest-derived tumor cell line Paju, which can be induced to differentiation in vitro by treatment with phorbol 12-myristate 13-acetate. Addition of the highly toxic potassium ionophores cereulide (4.5 and 9.0 ng/ml) or valinomycin (20 ng/ml), to cultures of undifferenti­ated Paju cells caused collapse of the mitochondrial membrane potential — measured with the fluorescent probe 5,5',6,6'-tetrachloro-1,1',3,3'-tetrabenzimidazole carbo- cyanine iodide (JC-1) followed by detachment of the cells and their apoptotic death. After induced differentiation of the Paju cells, their mitochondria retained the mem­brane potential upon exposure to the toxins and the cells displayed increased resis­tance to apoptosis as compared with undifferentiated cells. This effect may be caused by an elevated expression of the anti-apoptotic protein Bcl-2 and of the neuroprotective factor, stanniocalcin, in differentiated cells.

Słowa kluczowe

EN

membrane potential; Paju cell; mitochondrion; neuronal cell; potassium ionophore; cereulide; cell differentiation; toxic effect; valinomycin

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2004
• Tom: 51
• Numer: 2
• Opis fizyczny: p.539-544,fig.,ref.

Twórcy

autor

Teplova V.

• Russian Academy of Sciences, RU-142290 Pushchino, Moscow Region, Russia

autor

Jaaskelainen E.

autor

Salkinoja-Salonen M.

autor

Saris N.E.L.

autor

Serlachius M.

autor

Li F.Y.

autor

Andersson L.C.

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