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2004 | 51 | 2 |
Tytuł artykułu

Differentiated Paju cells have increased resistance to toxic effects of potassium ionophores

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
In this study we have investigated the impact of differentiation of neuronal cells on their sensitivity to microbial toxins. We used the human neural crest-derived tumor cell line Paju, which can be induced to differentiation in vitro by treatment with phorbol 12-myristate 13-acetate. Addition of the highly toxic potassium ionophores cereulide (4.5 and 9.0 ng/ml) or valinomycin (20 ng/ml), to cultures of undifferenti­ated Paju cells caused collapse of the mitochondrial membrane potential — measured with the fluorescent probe 5,5',6,6'-tetrachloro-1,1',3,3'-tetrabenzimidazole carbo- cyanine iodide (JC-1) followed by detachment of the cells and their apoptotic death. After induced differentiation of the Paju cells, their mitochondria retained the mem­brane potential upon exposure to the toxins and the cells displayed increased resis­tance to apoptosis as compared with undifferentiated cells. This effect may be caused by an elevated expression of the anti-apoptotic protein Bcl-2 and of the neuroprotective factor, stanniocalcin, in differentiated cells.
Wydawca
-
Rocznik
Tom
51
Numer
2
Opis fizyczny
p.539-544,fig.,ref.
Twórcy
autor
  • Russian Academy of Sciences, RU-142290 Pushchino, Moscow Region, Russia
autor
autor
Bibliografia
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-article-8e557117-2306-45ea-aaa3-4dfe084733ab
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