Uptake of photofrin II, a photosensitizer used in photodynamic therapy, by tumour cells in vitro

• Autorzy: Chwilkowska A. , Saczko J. , Modrzycka T. , Marcinkowska A. , Malarska A. , Bielewicz J. , Patalas D. , Banas T.
• Języki publikacji: EN

Abstrakty

EN

Photosensitizing dyes are used in fluorescence diagnostics and photodynamic ther­apy (PDT). These usually hematoporphyrin derivatives (HpD) accumulate preferen­tially within neoplastic tissues. HpD is a mixture of ether and ester linked porphyrins. Its partially purified form is known under the commercial name of photofrin II (PII). PII emission spectra were studied in a hydrophilic (PBS) and a lipophilic (PC liposomes) environment. Red shift was observed in their emission maxima from 615 nm in buffer solution to 635 nm in lipid. Identical red shift was obtained when the intracellular fluorescence of two cancer cell lines, MCF 7 and Jurkat, incubated with PII was investigated. Thus, intramembrane localization of PII may be suggested. As determined from the total fluorescence intensity, the uptake of PII was only slightly higher for Jurkat than for MCF 7 cells. Nevertheless the kinetics of the uptake was found to be different for both cell lines.

Słowa kluczowe

EN

fluorescence spectrum; hematoporphyrin derivative; tumour cell; treatment; neoplastic tissue; fluorescence diagnostics; photodynamic therapy; in vitro; cancer

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2003
• Tom: 50
• Numer: 2
• Opis fizyczny: p.509-513,fig.

Twórcy

autor

Chwilkowska A.

• Wroclaw Medical University, T.Chalubinskiego 10, 50-368 Wroclaw, Poland

autor

Saczko J.

autor

Modrzycka T.

autor

Marcinkowska A.

autor

Malarska A.

autor

Bielewicz J.

autor

Patalas D.

autor

Banas T.

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