Molecular analysis of bacterial community diversity in sequencing batch reactor [SBR] operating in autotrophic conditions

• Autorzy: Ciesielski S , Cydzik-Kwiatkowska A. , Turek J.
• Języki publikacji: EN

Abstrakty

EN

In the present study the bacterial community structure in a sequencing batch reactor (SBR) operating in autotrophic conditions was determined in relation to temporal variations of NH₄-N, NO₂-N, NO₃-N levels in the effluent. Bacterial richness and composition were determined by PCR-DGGE (Polymerase Chain Reaction - Denaturing Gradient Gel Electrophoresis) of 16S rRNA and amoA genes, and RISA (Ribosomal Intergenic Spacer Analysis) technique. The applied approaches revealed that the composition of bacterial population in the studied SBR varied in time. A positive correlation between Shannon index of ammonia-oxidizing bacteria (AOB) diversity and ammonia level in the effluent was observed. The variation of total bacterial diversity did not reflect the changes in nitrification efficiency.

Słowa kluczowe

EN

ammonia-oxidizing bacteria; sequencing batch reactor; bacteria; nitrification; polymerase chain reaction; bacterial community; denaturing gradient gel electrophoresis

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2007
• Tom: 56
• Numer: 1
• Opis fizyczny: p.45-51,fig.,ref.

Twórcy

autor

Ciesielski S

• University of Warmia and Mazury in Olsztyn, Sloneczna 45G, 10-718 Olsztyn, Poland

autor

Cydzik-Kwiatkowska A.

autor

Turek J.

Bibliografia

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