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2005 | 52 | 3 |

Tytuł artykułu

Nutritional properties of tubers of conventionally bred and transgenic lines of potato resistant to necrotic strain of potato virus Y [PVYn]

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The potential effect of genetic modification on nutritional properties of potatoes transformed to improve resistance to a necrotic strain of Potato virus Y was determined in a rat experiment. Autoclaved tubers from four transgenic lines were included to a diet in the amount of 40% and compared with the conventional cv. Irga. The experiment lasted 3 weeks and special attention was paid to nutritional properties of diets, caecal metabolism and serum indices. Genetic modification of potato had no negative effect on the chemical composition and nutritional properties of tubers, ecosystem of the caecum, activity of serum enzymes and non-specific defence mechanism of the rats. Obtained results indicate that transgenic potato with improved resistance to PVYN: line R1F (truncated gene coding for PVYN polymerase in sense orientation), R2P (truncated gene coding for PVYN polymerase in antisense orientation), and NTR1.16 (non-translated regions of PVYN genome in sense orientation) are substantial and nutritional equivalence to the non-transgenic cultivar. Tubers of transgenic line NTR2.27 (non-translated regions of PVYN genome in antisense orientation) increased the bulk of caecal digesta and the production of SCFA as compared to tubers of the conventional cultivar and the other transgenic clones. Taking into account some deviations, it seems reasonable to undertake a long-term feeding study to confirm the nutritional properties of tubers of transgenic lines.

Wydawca

-

Rocznik

Tom

52

Numer

3

Opis fizyczny

p.725-729,ref.

Twórcy

  • Polish Academy of Sciences, Olsztyn, Poland
autor
autor

Bibliografia

  • Aumaitre A, Aurlich K, Chesson A, Flachowsky G, Piva G (2000) New feeds from genetically modified plants: Substantial equivalence, nutritional equivalence, digestibility, and safety for animals and the food chain. Livest Prod Sci 74: 223–238.
  • Beever DE, Kemp CF (2000) Safety issues associated with DNA in animal feed derived from genetically modified crops. A review of scientific and regulatory procedures. Nutr Abstr Rev Ser B 70: 175–182.
  • Böhme H, Hommel B, Flachowsky G (2005) Nutritional assesment of silage from transgenic inulin synthesis potatoes for pigs. J Anim Feed Sci 14 (Suppl. 1): 333–336.
  • Broll H, Zagon J, Butschke A, Leffke A, Spiegelberg A, Böhme H, Flachowsky G (2005) The fate of DNA of transgenic inulin synthesizing potato in pigs. J Anim Feed Sci 14 (Suppl 1): 337–340.
  • Chachulska AM, Chrzanowska M, Flis B, Krzymowska M, Lipska-Dwużnik A, Robaglia C, Zagórski W (1997) Potato and tobacco cultivars transformation towards potato virus resistance. Biotechnologia 4: 48–54.
  • Chrzanowska M, Doroszewska T (1997) Comparison between PVY isolates obtained from potato and tobacco plants grown in Poland. Phytopathol Polon 8: 15–20.
  • Flachowsky G, Böhme H (2005) Proposal for nutritional assessment of feeds for genetically modifies plants. J Anim Feed Sci 14 (Suppl. 1): 49–70.
  • Flis B, Zimnoch-Guzowska E (2000) Field performance of transgenic clones obtained from potato cv. Irga. J Appl Genet 41: 81–90.
  • Gazendam I, Oelofse D, Berer DK (2004) High-level expression of apple PGIP1 is not sufficient to protect transgenic potato against Verticillum dahliae. Physiol Mol Plant 65: 145–155.
  • Hashimoto W, Momma K, Katsube T, Ohkawa Y, Ishige T, Kito M, Utsumi S, Murata K (1999a) Safety assessment of genetically engineered potatoes with designed soybean glycinin: compositional analyses of the potato tubers and digestibility of the newly expressed protein in transgenic potatoes. J Sci Food Agric 79: 1607–1612.
  • Hashimoto W, Momma K, Yoon HJ, Ozawa S, Ohkawa Y, Ishige T, Kito M, Utsumi S, Murata K (1999b) Safety assessment of transgenic potatoes with soybean glycinin by feeding studies in rats. Biosci Biotechn Biochem 63: 1942–1946.
  • Jondedijk E, De Schutter AAJM, Sollte T, van den Elzen PJM, Cornelissen BJC (1992) Increased resistance to potato virus X and preservation of cultivar properties in transgenic potato under field condition. Bio/Technology 10: 422–429.
  • Juśkiewicz J, Zduńczyk Z, Frejnagel S, Fornal J (2004) Caecal fermentation in rats fed diets containing tubers of transgenic potato. J Animal Feed Sci 13 (Suppl 2): 93–96.
  • Kosieradzka I, Sawosz E, Pastuszewska B, Zuk M, Szopa J, Bielecki W (2004) Effect of feeding potato tubers modified by 14-3-3 protein overexpession on metabolism and health status of rats. J Anim Feed Sci 13: 329–339.
  • Missiou A, Kalantidis K, Boutla A, Tzortzakaki S, Tabler M, Tsagris M (2004) Generation of transgenic potato plants highly resistant to Potato virus Y (PVY) through RNA silencing. Mol Breeding 14: 185–197.
  • Prescha A, Biernat J, Szopa J (2002) Quantitative and qualitive analysis of lipids in genetically modified potato tubers with varying rates of 14-3-3 protein synthesis. Nahrung 46: 179–183.
  • Rogan GJ, Bookout JT, Duncan DR, Fuchs RL, Lavrik PB, Love SL, Mueth M, Olson T, Owens ED, Raymond PJ, Zalewski J (2000) Compositional analysis of tubers from insect and virus resistant potato plants. J Agric Food Chem 48: 5936–5945.
  • Sanhoty L, EL-Rahman AA, Bolg KW (2004) Quaity and safety evaluation of genetically modified potatoes spunta with CryV gene: compositional analysis, determination of some toxins, antinutriens compounds and feeding study in rats. Nathrung 48: 8–13.
  • Zduńczyk Z (2001) In vivo experiments on the safety evaluation of GM components of feeds and foods. J Anim Feed Sci 10 (Suppl. 1): 195–210.
  • Zduńczyk Z, Frejnagel S, Fornal J, Flis M, Palacios MC, Flis B, Zagórski-Ostoja W (2005a) Biological response of rat fed diets with high tuber content of conventionally bred and transgenic potato resistant to necrotic strain of Potato virus Y (PVYN). Part. I. Chemical composition of tubers and nutritional value of diets. Food Control 16: 761–766.
  • Zduńczyk Z, Juśkiewicz J, Fornal J, Mazur-Gonkowska B, Koncicki A, Flis B, Zimnoch-Guzowska E, Zagórski-Ostoja W (2005b) Biological response of rat fed diets with high tuber content of conventionally bred and transgenic potato resistant to necrotic strain of Potato virus Y (PVYN). Part. II. Caecal metabolism, serum enzymes and indices of non-specific defence of rats. Food Control 16: 767–772.
  • Zuk M, Prescha A, Kępczyński J, Szopa J (2003) ADP ribosylation factor regulates matabolism and antioxidant capacity of transgenic potato tubers. J Agric Food Chem 51: 288–294.
  • Zuk M, Weber R, Szopa J (2005) 14-3-3 protein down-regulates key enzyme activities of nitrate and carbohydrate metabolism in potato plants. J Agric Food Chem 53: 3454–3460.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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