Enrichment, isolation and susceptibility profile of the growth substrate of bacterial strains able to degrade vinyl acetate

• Autorzy: Gren I , Gaszczak A , Szczyrba E , Labuzek S
• Języki publikacji: EN

Abstrakty

EN

Nowadays biodegradations of harmful xenobiotics seems to be the best and cheapest method of purification of the polluted environment. VOCs are represented by vinyl acetate, which is thought to be carcinogenic. The aim of these studies was to isolate and determine the susceptibility profile for vinyl acetate of bacterial strains. The source of microorganisms was soil sampled in the area of Synthos S.A. in Oświęcim, Poland. From among 41 isolates, 4 Gram-negative strains were chosen for further analyses. As the control, one laboratory strain of Pseudomonas fluorescens PCM 2123 from The Polish Collection of Microorganisms (Wrocław) was used. Simultaneously, a susceptibility profile to vinyl acetate was performed on Stenotrophomonas maltophilia KB2 strain, aromatic compounds’ degrader. Vinyl acetate used in concentration of 3,000 ppm inhibited growth of gram-positive bacteria, and 4,000 ppm was the lethal dose for microorganisms from mixed populations. A toxicity test showed susceptibility to vinyl acetate at concentrations of 2,000 ppm. Three weeks of pre-incubation with 400 ppm of vinyl acetate magnified the level of sensitivity to 3,000 ppm of vinyl acetate for almost all strains. Although decomposition of vinyl acetate was observed even in the presence of 4,000, 5,000 and 6,000 ppm of vinyl acetate, growth was not observed. It was due to enlarged concentration of acetaldehyde, a product of hydrolysis ester bond of vinyl acetate.

Słowa kluczowe

EN

vinyl acetate; volatile organic compound; environment pollution; biodegradation; isolation; susceptibility profile; bacterial strain; growth substrate; acetaldehyde; toxicity; Pseudomonas; Stenotrophomonas

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2009
• Tom: 18
• Numer: 3
• Opis fizyczny: p.383-390,fig.,ref.

Twórcy

autor

Gren I

• Department of Biochemistry, Faculty of Biology and Environment Protection, University of Silesia, Jagiellonska 28, 40-032 Katowice, Poland

autor

Gaszczak A

• Institute of Chemical Engineering, Polish Academy of Sciences, Baltycka 5, 44-100 Gliwice, Poland

autor

Szczyrba E

• Institute of Chemical Engineering, Polish Academy of Sciences, Baltycka 5, 44-100 Gliwice, Poland

autor

Labuzek S

• Department of Biochemistry, Faculty of Biology and Environment Protection, University of Silesia, Jagiellonska 28, 40-032 Katowice, Poland

Bibliografia

• 1. MIN K., ERGAS S.J. Volatilization and biodegradation of VOCs in membrane bioreactors. J. Water, Air, and Soil Pollution, 6, 83, 2006.
• 2. NIEDER M., SUNARKO B., MEYER O. Degradation of vinyl acetate by soil, sewage, sludge, and the newly isolated aerobic bacterium V2. Appl. Environ. Microbiol. 56, 3023, 1990.
• 3. HATANAKA Y., INOUE Y., MURATA K., KIMURA A. Isolation and characterization of carboxylesterase from vinyl acetate-assimilating bacterium isolated from soil. J. Ferment. Bioeng. 67(1), 14, 1989.
• 4. WALLACE R.J., CHAUDHARY L.C., MIYAGAWA E., MCKAIN N., WALKER N.D. Metabolic properties of Eubacterium pyruvativorans, a ruminal ‘hyper-ammonia producing’ anaerobe with metabolic properties analogous to those of Clostridium kluyveri. Microbiology. 150, 2921, 2004.
• 5. LIU S-Q., HOLLAND R., CROW V.L. Purification and properties of intracellular esterases from Streptococcus thermophilus. Int. Dairy J. 11, 27, 2001.
• 6. SMACCHI E., GOBBETTI M., ROSSI J., FOX P.F. Purification and characterization of an extracellular esterase from Arthrobacter nicotianae 9458. Lait. 80, 255, 2000.
• 7. ALMEIDA R.V., ALQUERES S.M.C., LARENTIS A.L., RÖSSLE S.C., CARDOSO A.M., ALMEIDA W.I., BISH P.M., ALVES T.L.M., MARTINS O.B. Cloning, expression, partial characterization and structural modeling of a novel esterase from Pyrococcus furiosus. Enzyme Microb. Technol. 39, 1128, 2006.
• 8. ATESLIER Z.B.B., METIN K. Production and partial characterization of a novel thermostable esterase from a thermophilic Bacillus sp. Enzyme Microb. Technol. 38, 628, 2006.
• 9. BOGDANFFY M.S., SARANGAPANI R., KIMBELL J.S., FRAME R., PLOWCHALK D.R. Analysis of vinyl acetate metabolism in rat and human nasal tissues by an in vitro gas uptake technique. Toxicol. Sci. 46, 235, 1998.
• 10. BOGDANFFY M.S., TAYLOR M.L. Kinetics of nasal carboxyloesterase- mediated metabolism of vinyl acetate. Drug Metab. Dispos. 21, 1107, 1993.
• 11. MORRIS J.B., SYMANOWICZ P., SARANGAPANI R. Regional distribution and kinetics of vinyl acetate hydrolysis in the oral cavity of the rat and mouse. Toxicol. Lett. 126, 31, 2002.
• 12. JAKOBY W.B., NARROD S.A. Aldehyde oxidation IV. An aldehyde buffer for growth studies. J. Bacteriol. 77, 410, 1958.
• 13. KASPERCZYK D., BARTELMUS G., KLEPACKA K., GĄSZCZAK A. Biological purification of air streams contaminated with vinyl acetate in the trickle bed reactor, Przem. Chem. 86, 968, 2007 [In Polish].
• 14. GUZIK U. Biochemical and genetic characterization of dioxygenases, involved in degradation of aromatic compounds, from bacterial strains. Doctoral thesis, Silesian University, Katowice, 2007 [In Polish].
• 15. http://esc.syrres.com/scripts/CHEMLISTcgi.exe
• 16. MARKIEWICZ Z. Structure and function of bacterial cell envelope. WN PWN: Warsaw, pp. 141-199, 1993 [In Polish].
• 17. BUITRON G., CAPDEVILLE B. Enhancement of the biodegradation activity by the acclimation of the inoculum. Environ. Technol. 16, 1175, 1995.
• 18. Van DER MEER J.R., VOS W.M., HARAYAMA S., ZEHDNER A.J.B. Molecular mechanisms of genetic adaptation to xenobiotic compounds. Microbiol. Rev. 56, 677, 1992.
• 19. CORNELISSEN G., SIJM D.T.H.M. An energy budget model for the biodegradation and cometabolism of organic substrate. Chemosphere. 33, 817, 1996.
• 20. INGUVA S., SHREVE G.S. Biodegradation kinetics of trichloroethylene and 1,2-dichloroethane by Burkholderia (Pseudomonas) cepacia PR131 and Xanthobacter autotrophicus GJ109. Int. Biodeterior. Biodegrad. 43, 57, 1999