The influence of culture conditions on adhesion of Listeria monocytogenes to hexadecane

• Autorzy: Dabrowski W. , Iwanski R. , Czeszejko K. , Medrala D.
• Języki publikacji: EN

Abstrakty

EN

Results of various culture conditions on adhesion of Listeria monocytogenes strains to hexadecane are presented. Listeria strains cultured in optimal conditions adhered to this hydrophobic carbohydrate to a small extent. It reveals that their cell walls have hydrophilic properties. Application of low-nutrient medium, incubation at low temperature, acidification of medium to pH 5 and addition of 8% of NaCl caused transformation of strains into hydrophobic. Growing in vacuum conditions produced the opposite effect – all strains were extremely hydrophilic. Changes of strain properties from hydrophilic into hydrophobic affected analysed strains to a different degree. One of tested strains continued to be hydrophilic regardless of culture conditions. The other – a standard strain isolated from patient – became hydrophobic most frequently.

Słowa kluczowe

EN

hydrophobicity; adhesion; Listeria monocytogenes; culture condition

• Wydawca: -
• Czasopismo: Electronic Journal of Polish Agricultural Universities. Series Food Science and Technology
• Rocznik: 2001
• Tom: 04
• Numer: 2
• Opis fizyczny: http://www.ejpau.media.pl

Twórcy

autor

Dabrowski W.

• Agricultural University of Szczecin, Papieza Pawla VI 3, 71-459 Szczecin, Poland

autor

Iwanski R.

autor

Czeszejko K.

autor

Medrala D.

Bibliografia

• Aarts H.J.M., Hakemulder L.E., Van Hoef A.M.A., 1999, Genomic typing of Listeria monocytogenes strains by automated laser fluorescence analysis of amplified fragment length polymorphism fingerprint patterns. Int. J. Food Microbiol. 49: 95-102.
• Arino S., Marchal R., Vandecasteele J.P., 1998, Involvement of a rhamnolipid-producing strain of Pseudomonas Aeruginosa in the degradation of polycyclic aromatic hydrocarbons by a bacterial community. J. Appl. Microbiol. 84: 769-776.
• Bayles D. O., Annous B. A., Wilkinson B. J., 1996, Cold stress proteins induced in Listeria monocytogenes in response to temperature downshock and growth at low temperatures. Appl. Env. Microbiol. 62: 1116-1119.
• Beachey E.H., 1981, J. Infect. Dis. 143: 325.
• Brosch R., Catimel B., Milon G., Buchrieser C., Vindel E., Rocourt J., 1993, Virulence heterogeneity of Listeria monocytogenes strains from various sources (food, human, animal) in immunocompetent mice and its association with typing characteristics. J. Food Prot. 56: 296-301.
• Dąbrowski W., Iwański R., Bednarska M., Mędrala D., 2001, The effect of culture condictions of hydrophobicity of strains of Listeria monocytogenes isolated from food. EJPAU.
• Dickson J.S., Koohmaraie M., 1989, Cell surface charge characteristics and their relationship to bacterial attachment to meat surfaces. Appl. Env. Microbiol. Apr. 55, 4: 832-836.
• Heinitz M.L., Johnson J.M., 1998, The incidence of Listeria spp., Salmonella spp. and Clostridium botulinum in smoked fish and shellfish. J. Food Prot. 61: 318-323.
• Hoštacka A., Šipošova E., 1998, Klebsiella pneumoniae clinical isolates: susceptibility to some antibiotics and surface hydrophobicity. Microbios 95: 101-107.
• Jankowski S., Sarowska J., Zarczyńska H., Cisowska A., 1997: Hydrophobic properties of Pseudomonas aeruginosa strains. Med. Dośw.. Mikrobiol. 49: 187-190.[in Polish]
• Kwiatek K., 1993, Appeareance of L. monocytogenes in meat and meat products, Życie Wet. 12: 304-306, [in Polish].
• Ławrynowicz I., Tylewska S., Hryniewicz W., 1985, Physicochemical properties of cell surfaces of Streptococcus agalactiae. Med. Dośw. Mikrobiol. 37: 131. [in Polish]
• Maisnier-Patin S., Richard J., 1996, Cell wall changes in nisin-resistant variants of Listeria innocua grown in the presence of high nisin concentrations. FEMS Microbiol. Lett., 140(1): 29-35.
• Miller A. L., Smith J. L., Somkuti G. A., 1990, Foodborne Listeriosis. ELSEVIER, 5: 25.
• Santiago N.I., Zipf A., Bhunia A.K., 1999, Influence of temperature and growth phase on expression of 104-kilodalton Listeria adhesion protein in Listeria monocytogenes. Appl. Environ. Microbiol. 65: 2765-2769.
• Takeuchi K., Matute C.M., Hassan A.N., Frank J.F., 2000, Comparison of attachment of Escherichia coli O157:H7, Listeria monocytogenes, Salmonella typhimurium, and Pseudomonas fluorescens to lettuce leaves. J. Food Prot. 63: 1433-1437.
• Van der Mei H.C., De Vries J., Busscher H.J., 1993, Hydrophobic and electrostatic cell surface properties of thermophilic sairy Streptococci. Appl. Env. Microbiol. 59, 12: 4305-4312.