Ultrastructural and autoradiographic study of Chara vulgaris Manubria

• Autorzy: Kwiatkowska M. , Wojtczak A. , Poplonska K.
• Języki publikacji: EN

Abstrakty

EN

Ultrastructural changes of manubria of Chara vulgaris during spermiogenesis were studied. The changes (as compared to the antheridial filament proliferation stage) involve thickening of the content of secretive vesicles and changes in their consistency, the disappearance of rough endoplasmic reticulum (ER) and appearance of smooth ER, increases in the number and size of plastoglobules in plastids, condensation of mitochondria, and significant cell vacuolization. Autoradiography with 3H-fucose pulse incubation showed that during spermatogenesis it was incorporated into the manubria. Their radioactivity decreased after 2 h postincubation in nonradioactive fucose during spermiogenesis. This was not observed during proliferation of antheridial filament cells. It is suggested that polysaccharide secretion into the internal antheridial space increases during spermiogenesis, perhaps connected with the thickening of secretive vesicle content observed by electron microscopy.

Słowa kluczowe

EN

alga; Chara vulgaris; ultrastructural change; Charophyta; antheridium; 3H-fucose; manubrium; spermatogenesis; ultrastructure; spermiogenesis

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2002
• Tom: 44
• Opis fizyczny: p.99-105,fig.,ref.

Twórcy

autor

Kwiatkowska M.

• University of Lodz, Pilarskiego 14, 90-231 Lodz, Poland

autor

Wojtczak A.

autor

Poplonska K.

Bibliografia

• Cheung AG, and WU HM. 1999. Arabinogalactan proteins in plant sexual reproduction. Protoplasma 208: 87-98.
• Creelman RA, and Mullet JE. 1997. Oligosaccharins, brassinolides and jasmonates: Nontraditional regulators of plant growth development and gene expression. The Plant Cell 9: 1211-1223.
• Eltzler ME. 1998. Oligosaccharide signaling of plant cells. Journal of Cell Biochemistry Suppl. 30/31: 123-128.
• Fry SC, Aldington S, Hetherington PR, and Aitken J. 1993. Oligosaccharides as signals and substances in the plant cell wall. Plant Physiology 103: 1-5.
• Gillet C, and Liners F. 1996. Changes in distribution of short pectic polysaccharides induced by monovalent ions in the Nitella cell wall. Canadian Journal of Botany 74: 26-30.
• Gori P. 1982. Accumulation of polysaccharides in the anther cavity of Allium sativum clone Piemonte. Journal of Ultrastructure Research 81: 152-163.
• Gosek A. 1993. Comparative studies on the amount and concentration of antheridial mucilage during the successive stages of the spermatogenesis in Chara tomentosa and Chara contraria. Bulletin of the Polish Academy of Sciences. Biological Sciences 41: 1-11.
• Gosek A. 1996. Autoradiographic studies on mucilage synthesis in Chara vulgaris antheridium with the use of 3H-fucose in total darkness and light. Folia Histochemica et Cytobiologica 34: 155-160.
• Gosek A, and Kwiatkowska M. 1991. Cytochemical studies on the antheridial mucilage and changes in its concentration and amount during the spermatogenesis in Chara vulgaris L. Folia Histochemica et Cytobiologica 29: 91-100.
• Gosek A, and Kwiatkowska M. 1993. Autoradiographic studies on 3H-fucose incorporation into manubria and its translocation to antheridial space during spermatogenesis in Chara vulgaris L. Folia Histochemica et Cytobiologica 31: 233-237.
• Kwiatkowska M, and Maszewski J. 1987a. Ultrastructural and autoradiographic studies of non-generative cells in the antheridium of Chara vulgaris L. I. Manubria. Folia Histochemica et Cytobiologica 25: 215-224.
• Kwiatkowska M, and Maszewski J. 1987b. Ultrastructural and autoradiographic studies of non-generative cells in the antheridium of Chara vulgaris L. II. Capitular cells. Folia Histochemica et Cytobiologica 25: 225-232.
• Kwiatkowska M, and Popłońska K. 2002. Further ultrastructural research of Chara vulgaris spermiogenesis: endoplasmic reticulum, structure of chromatin, 3H-lysine and 3H-arginine incorporation. Folia Histochemica et Cytobiologica 40: 1-13.
• Kwiatkowska M, and Żylińska K. 1988. Ultrastructural and autoradiographic studies of non-generative cells in the antheridium of Chara vulgaris L. III. Shield cells. Folia Histochemica et Cytobiologica 26: 225-236.
• Kwiatkowska M, Popłońska K, and Żylińska K. 1990. Biological role of endoreplication in the process of spermatogenesis in Chara vulgaris L. Protoplasma 155: 176-187.
• Langan KJ, and Nothnagel EA. 1997. Cell surface arabinogalactan-proteins and their relation to cell proliferation and viability. Protoplasma 196: 87-98.
• Morrison JC, Greeve LC, and Richmond PA. 1993. Cell wall synthesis during growth and maturation of Nitella internodal cells. Planta 189: 321-328.
• Olszewska MJ. 1978. Chromatin condensation in late G2 phase as a factor related to the earlier initiation of mitosis in successive generations of antheridial filaments of Chara vulgaris L. Microscopica Acta 80: 115-126.
• Olszewska MJ, Gernand D, Godlewski M, and Kunachowicz A. 1997. DNA - méthylation during antheridial filament development and spermiogenesis in Chara vulgaris (Charo- phycae) analysed by in situ nick-translation driven by methylation - sensitive restriction enzymes. European Journal of Phycology 32: 287-291.
• Olszewska MJ, and Godlewski M. 1972. An autoradiographic study of the synthesis of nucleic acids and protein during the cell cycle of synchronously dividing antheridial filaments in Chara vulgaris L. Folia Histochemica et Cytochemica 10: 245-256.
• Pickett-Heaps JD. 1968. Ultrastructure and differentiation in Chara sp. III Formation of the antheridium. Australian Journal of Biological Sciences 21: 255-274.
• Reynolds SS. 1963. The use of lead citrate of high pH as an electron-opaque stain in electron microscopy. Journal of Cell Biology 17: 208-212.
• Rougier M, and Chaboud A. 1985. Mucilages secreted by roots and their biological functions. Israel Journal of Botany 34: 129-146.
• Warneck H, and Seitz HV. 1993. Inhibition of gibberellic acid-induced elongation-growth of pea epicotyls by xyloglucan oligosaccharides. Journal of Experimental Botany 44: 1105-1109.