Immunofluorescent detection of CD15-fucosylated glycoconjugates in primary cerebellar cultures and their function in glial-neuronal adhesion in the central nervous system

• Autorzy: Sajdel-Sulkowska E. M.
• Języki publikacji: EN

Abstrakty

EN

Expression of CD15 antigen (also referred to as stage specific embryonic antigen, SSEA-1, or Lewisx) was analyzed in cerebellar cultures prepared from seven day old rats by double immunostaining with anti-CD15 mAb7A and cell-specific antibody glial fibrillary acidic protein (GFAP) and Vimentin. The immunocytochemical data suggest that the expression of CD15 antigen is restricted to some GFAP-positive cells with fibroblast-like morphology characteristic of Type-1 astrocytes. In order to explore the involvement of CD15 antigen in glial-neuronal interactions, the ability of mAb7A antibody to interfere with granule cell adhesion to a monolayer of astrocytes was tested in comparison with anti-GFAP. The adhesion of cerebellar granule cells to astrocytes, as determined by the number of bound cells, was decreased by 39% following preincubation with mAb7A. Anti-GFAP did not alter cell adhesion, indicating the specificity of the anti-CD15 antibody effect. These results are consistent with the hypothesis that CD15 antigen participates in glial-neuronal interactions in the developing cerebellum. Furthermore, it may be speculated that the modulation of cell-surface CD15 expression contributes to the altered strength of glial-neuronal interaction, facilitating cell migration and differentiation.

Słowa kluczowe

EN

glial-neuronal adhesion; astrocyte; glial-neuronal interaction; cerebellum; cerebellar cell; CD15 antigen; cell migration; rat; central nervous system; cerebellar culture; cell adhesion

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1998
• Tom: 45
• Numer: 3
• Opis fizyczny: p.781-790,fig.

Twórcy

autor

Sajdel-Sulkowska E. M.

• Brigham and Women's Hospital, Boston, MA 02115, USA

Bibliografia

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