Detection of the status of tumour-infiltrating CD4plus T-cell subpopulations in squamous cell carcinoma of the head and neck [HNSCC] using real-time RT-PCR detection of CD4 mRNA

• Autorzy: Smerkolj S , Volavsek M. , Glavac D.
• Języki publikacji: EN

Abstrakty

EN

Several studies have reported tumour infiltrating CD4+ T cells as a favourable prognostic factor in some types of cancer. We investigated 37 head and neck squamous cell carcinomas (HNSCC) at different stages, using immunohistochemical staining for CD4+ infiltrates and real-time reverse transcription polymerase chain reaction (RT-PCR) detection of CD4 mRNA. The CD4+ infiltrates were evaluated and expressed as a percentage according to the ratio of CD4+ T cells to epithelial cells in the cancer cell nests and to the overall inflammatory cell infiltrate in the tumor stroma. The CD4 mRNA expression level strongly correlated with the CD4+ infiltration score in the cancer epithelium (rs = 0.858, P < 0.001) and in the cancer stroma (rs = 0.797, P < 0.001). These results indicate that the real-time RT-PCR assay is a sensitive and reliable method for the detection of CD4 mRNA, and that it could be used to reassess CD4+ infiltration status in resected specimens from patients with HNSCC.

Słowa kluczowe

EN

mRNA detection; cancer cell; epithelial cell; gene expression; squamous cell; neck; real-time reverse transcription polymerase chain reaction; head; T cell; subpopulation; human disease; patient; carcinoma; head and neck squamous cell carcinoma; polymerase chain reaction; detection

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2007
• Tom: 12
• Numer: 2
• Opis fizyczny: p.308-316,fig.,ref.

Twórcy

autor

Smerkolj S

• University of Ljubljana, Ljubljana, Slovenia

autor

Volavsek M.

autor

Glavac D.

Bibliografia

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