Effect of natural phenols on the catalytic activity of cytochrome P450 2E1

• Autorzy: Mikstacka R. , Gnojkowski J. , Baer-Dubowska W.
• Języki publikacji: EN

Abstrakty

EN

The effect of protocatechuic acid, tannic acid and trans-resveratrol on the activity of p-nitrophenol hydroxylase (PNPH), an enzymatic marker of CYP2E1, was examined in liver microsomes from acetone induced mice. trans-Resveratrol was found to be the most potent inhibitor (IC50 = 18.5 ± 0.4 uM) of PNPH, while protocatechuic acid had no effect on the enzyme activity. Tannic acid with IC50 = 29.6 ± 3.3 uM showed mixed- and trans-resveratrol competitive inhibition kinetics (Ki = 1 uM and 2.1 uM, respec­tively). Moreover, trans-resveratrol produced a NADPH-dependent loss of PNPH ac­tivity, suggesting mechanism-based CYP2E1 inactivation. These results indicate that trans-resveratrol and tannic acid may modulate cytochrome P450 2E1 and influence the metabolic activation of xenobiotics mediated by this P450 isoform.

Słowa kluczowe

EN

xenobiotic; phenol; catalytic activity; resveratrol; mechanism-based inhibition; dietary polyphenol; protocatechuic acid; vegetable; fruit; tannic acid; P450 isoform; metabolic activity; inhibition kinetics; natural phenol; polyphenol; cytochrome P450

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2002
• Tom: 49
• Numer: 4
• Opis fizyczny: p.917-925,fig.

Twórcy

autor

Mikstacka R.

• University of Medical Sciences, Grunwaldzka 6, 60-780 Poznan, Poland

autor

Gnojkowski J.

autor

Baer-Dubowska W.

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