Regulation of expression of stromyelysin-1 by proinflammatory cytokines in mouse brain astrocytes

• Autorzy: Witek-Zawada B. , Koj A.
• Języki publikacji: EN

Abstrakty

EN

Stromelysin-1 belongs to matrix metalloproteinases responsible for proteolytic degradation of extracellular matrix in many tissues during various diseases, especially those involving inflammation. We studied the induced expression of stromelysin-1 in primary cultures of mouse brain astrocytes stimulated with various cytokines and cellular growth factors. Interleukin-1-beta (IL-1ß), tumor necrosis factor alpha (TNFalpha) and a mixture of IL-1, TNF and epidermal growth factor (EGF) significantly increased the level of stromelysin-1 mRNA in mouse astrocytes while interferon-gamma (IFN-) inhibited this response or was without effect. This accumulation of specific mRNA was preceded by activation of two examined transcription factors: NFB and AP-1. However, experiments with known inhibitors of activation of these transcription factors: pyrrolidine dithiocarbamate (PDTC), parthenolide and curcumin, indicate that NFB and AP-1 cannot be solely responsible for the cytokine induced expression of stromelysin-1 gene in mouse astrocytes.

Słowa kluczowe

EN

epidermal growth factor; interleukin 1; mice; brain; astrocyte; matrix metalloproteinase; stromyelysin-1; tumour necrosis factor; expression; mouse; cytokine; interferon; regulation; mRNA

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2003
• Tom: 54
• Numer: 4
• Opis fizyczny: p.489-496,fig.,ref.

Twórcy

autor

Witek-Zawada B.

• Jagiellonian University, Gronostajowa 7, 30-384 Krakow, Poland

autor

Koj A.