PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2009 | 65 | 01 |

Tytuł artykułu

Hodowle in vitro pierwotniakow Babesia izolowanych od zwierzat

Warianty tytułu

EN
In vitro cultivation of Babesia organisms isolated from animals

Języki publikacji

PL

Abstrakty

EN
Babesia organisms are hemoprotozoa isolated from people and animals. Their in vitro cultivation supplies information about the morphology and ultrastructure of these parasites. They make it possible to obtain the genetic material for diagnostic elaborations and for detections of phylogenetic relationships between various species of Babesia. Protozoa cultivated on red blood cells can be a source of parasite antigen or attenuated strains of protozoa which can be used for vaccinations. The cultures of Babesia species are kept in an erythrocytes host, taken from the animals from which Babesia is most frequently isolated, suspended in tissue culture media with the addition of sera, in microaerophil conditions at 37°C. The specific circumstances and factors that are needed to obtain an effective Babesia culture are very often the reason of the failure of these investigations.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

65

Numer

01

Opis fizyczny

s.12-14,tab.,bibliogr.

Twórcy

autor
  • Uniwersytet Przyrodniczy w Lublinie, ul.Gleboka 30, 20-612 Lublin
autor

Bibliografia

  • 1.Abramowicz B.: Zaburzenia funkcji nerek i wątroby w przebiegu babeszjozy psów. Ann. UMCS, sec. DD 2007, 62, 80-86.
  • 2.Adaszek Ł., Winiarczyk S.: Molecular characterization of Babesia canis canis isolates from naturally infected dogs in Poland. Vet. Parasitol. 2008, 152, 235-241.
  • 3.Bautista C. R., Kreier J. P.: Effect of immune serum on the growth of Babesia microti in hamster erythrocytes in short term culture. Infect. Immun. 1979, 25, 470-472.
  • 4.Carcy B., Precigout E., Schetters T., Gorenflot A.: Genetic basis for GPI-anchor merozoite surface antigen polimophism of Babesia and resulting antigenic diversity. Vet. Parasitol. 2006, 138, 33-49.
  • 5.Erp E. E., Smith R. D., Ristic M., Osorno B. M.: Optimization of the suspension culture forin vitro culivation of Babesia bovis. Am. J. Vet. Res. 1980, 41, 2059-2062.
  • 6.Golf W. L., Yunker C. E.: Babesia bovis: increased percentage parasitized erythrocytes in cultures and assessment of growth by incorporation of [3H] hypoxanthine. Exp. Parasitol. 1986, 62, 202-210.
  • 7.Grande N., Precigout E., Ancelin L., Moubri K., Carcy B., Lemesre J. L., Vial H., Gorenflot A.: Continous in vitro culture of Babesia divergens in serum free medium. Parasitol. 1997, 115, 81-89.
  • 8.Gundłach L. J., Sadzikowski A. B., Tomczuk K.: Babeszjoza psów. Medycyna Wet. 1995, 51, 584-588.
  • 9.Hailat N. Q., Lafi S. Q., al-Darraji A. M., al-Ani F. K.: Equine babesiosis associated with strenuous exercise: clinical and pathological studies in Jordan. Vet. Parasitol. 1997, 69, 1-8.
  • 10.Holman P. J., Chieves L., Frerichs M., Olson D., Wagner G. G.: Babesia equi erythrocytic stage continously cultured in an enriched medium. J. Parasitol. 1994, 31, 232-236.
  • 11.Holman P. J., Waldrup K. A., Wagner G. C.: In vitro cultivation of a Babesia isolated from a white-tailed deer (Odocoileus virginianus). J. Parasitol. 1988, 74, 111-115.
  • 12.Kandefer-Szerszeń M.: Ćwiczenia z wirusologii. Wydawnictwo UMCS, Lublin 1997.
  • 13.Konrad J., Canning E. U., Phipp L. P., Donelly J.: Maintenance of in vitro cultures of Babesia divergens and Babesia major at low temperatures. Z. Parasitenkde 1985, 71, 313-316.
  • 14.Lehtinen L. E., Birkenheuer A. J., Droleskey R. E., Holman P. J.: In vitro cultivation of a newly recognized Babesia sp. in dogs in North Carolina. Vet. Parasitol. 2008, 151, 150-157.
  • 15.Levy M. G., Ristic M.: Babesia bovis continuous cultivation in a microaerophilus stationary phase culture. Science 1980, 207, 1218-1220.
  • 16.Levy M. G., Ristic M.: Cultivation and in vitro studies on Babesia, [w:] Jensen J. B. (ed.): In vitro Cultivation of Protozoan Parasites CRC Press, Boca Raton 1983, 221-224.
  • 17.Schetters T., Kleuskens J., Scholtes N., Pasman J. W., Bos H. J.: Vaccination of dogs against Babesia canis infection using antigens from culture supernatants with emphasis on clinical babesiosis. Vet. Parasitol. 1994, 52, 219-233.
  • 18.Schetters Th. P. M., Kleuskens J. A. G. M., Scholtes N. C., Gorenflot A., Moubri K., Vermeulen A. N.: Vaccination of dogs against heterologous Babesia canis infection using antigen from culture supernatants. Vet. Parasitol. 2001, 100, 75-86.
  • 19.Schuster F. L.: Cultivation of Babesia and Babesia-like blood parasites: agents of an emerging zoonotic disease. Clin. Microbiol. Rev. 2002, 15, 365-373.
  • 20.Smith T., Kilborne F. L.: Investigations into the nature, causation, and prevention of Texas or southern cattle fever, 8th and 9th Repts. Bur. Anim. Industr., U.S. Dept. Agric. 1893, 177-304.
  • 21.Tassi P., Carelli G., Ceci L.: Tick-borne diseases (TBDs) of dairy cows in a Mediterranean environment: a clinical, serological, and hematological study. Ann. N. Y. Acad. Sci. 2002, 969, 314-317.
  • 22.Uilenberg G.: Babesia - a historical overview. Vet. Parasitol. 2006, 138, 3-10.
  • 23.Yamasaki M., Asano H., Otsuka Y., Yamato O., Tajima M., Maede Y.: Use of canine red blood cellswith high concentrations of potassium reduced glutathione and free amino acid as host cells for in vitro cultivation of Babesia gibsoni. Am. J. Vet. Res. 2000, 61, 1520-1524.
  • 24.Zahler M., Schein E., Rinder H., Gothe R.: Characteristic genotypes discriminate between Babesia canis isolates of differing vector specificity and pathogenicity to dogs. Parasitol. Res. 1998, 84, 544-548.
  • 25.Zweygarth E., Just M. C., Waal D. T.: In vitro cultivation of Babesia equi: detection of carrier animals and isolation of parasites. Onderstepoort J. Vet. Res. 1997, 64, 51-56.
  • 26.Zweygarth E., Lopez-Rebolla L. M.: Continuous in vitro cultivation of Babesia gibsoni. Parasitol. Res. 2000, 86, 905-907.
  • 27.Zweygarth E., van Niekerk C. J., de Waal D. T.: Continuous in vitro cultivation of Babesia cabali in serum free medium. Parasitol. Res. 1999, 85, 413-416.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-894bc728-24a8-4aa4-abee-60ebaab5ccc2
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.