Determination of 10-deacetylbaccatine III in Taxus baccata needles by micellar electrokinetic chromatography

• Autorzy: Zarek M , Waligorski P.

Warianty tytułu

PL

Oznaczanie zawartosci 10-deacetylobakatyny III w iglach cisa pospolitego za pomoca micelarnej chromatografii elektrokinetycznej

• Języki publikacji: EN

Abstrakty

EN

The optimization of the analytical buffer composition for micellar electrokinetic chromatography in order to make simple separations of 10-deacetylbaccatine III from sample matrices (other taxanes and other compounds) and to determine its concentration in the samples was performed. The samples were prepared from crude Taxus baccata L. needle extracts. The best results were obtained with the buffer containing 25 mmol/dm3 Tris-HCl (pH 9.0), 40 mmol/dm3 sodium dodecyl sulphate and 15% acetonitrile for a wavelength 230 nm (Diode Array Detector). Finally determination of 10-DAB III concentration in T. baccata needles collected from four different populations of Southern Poland was performed (mean concentration respectively: Cisy w Nowej Wsi – 0.135 mg/g d.w., Cisowa Góra – 0.185 mg/g d.w., Zadni Gaj – 0.143 mg/g d.w., Cisy nad Liswartą – 0.150 mg/g d.w.). There were no significant differences in the mean concentration of 10-deacetylbaccatine III among samples from different populations.

PL

W prezentowanej pracy przeprowadzono optymalizację składu buforu umożliwiającego proste i szybkie rozdzielenie 10-deacetylobakatyny III od innych składników oraz oznaczenie jej ilości w analizowanych próbkach igieł cisa pospolitego za pomocą micelarnej chromatografii elektrokinetycznej. Najlepsze rezultaty uzyskano stosując bufor rozdziałowy zawierający: 25 mmol/dm3 Tris-HCl (pH 9,0), 40 mmol/dm3 dodecylosiarczanu sodu (SDS) i 15% acetonitrylu oraz dokonując odczytu przy długości fali 230 nm (Diode Array Detector). W celu sprawdzenia możliwości wykorzystania opracowanej metody przeprowadzono analizę zawartości 10-DAB III w igłach osobników rosnących w 4 rezerwatach w Polsce południowej. Średnie stężenie badanego związku wynosiło odpowiednio: Cisy w Nowej Wsi – 0,135 mg, Cisowa Góra – 0,185 mg, Zadni Gaj – 0,143 mg, Cisy nad Liswartą – 0,150 mg na gram suchej masy. Nie stwierdzono istotnych różnic między średnimi wartościami uzyskanymi dla poszczególnych populacji.

Słowa kluczowe

EN

yew; Taxus baccata; needle; micellar electrokinetic chromatography; 10-deacetylbaccatin; diode array detection; buffer; determination

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2009
• Tom: 55
• Numer: 2
• Opis fizyczny: p.25-35,fig.,ref.

Twórcy

autor

Zarek M

• Agricultural University, 29 Listopada 46, 31-425 Krakow, Poland

autor

Waligorski P.

Bibliografia

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