The effect of carbohydrates and polyethylene glycol on somatic embryo maturation in hybrid fir Abies alba x Abies numidica

• Autorzy: Salaj T , Matusova R. , Salaj J.
• Języki publikacji: EN

Abstrakty

EN

Embryogenic cell line AN 72 derived from immature hybrid fir Abies alba x A. numidica zygotic embryos was subjected to different maturation treatments. The effect of the carbohydrates sucrose, maltose and glucose (each at 3%, 6% and 9%) or PEG-4000 (5.0%, 7.5% and 10%) combined with different carbohydrate sources was tested. PEG-4000 stimulated somatic embryo maturation of hybrid fir. This stimulatory effect was dependent on the carbohydrate source used. Culture medium with maltose as carbohydrate source combined with PEG-4000 produced the highest number of cotyledonary somatic embryos. Carbohydrates supplied alone (mainly at 6% and 9%) exerted an unfavorable effect, increasing the frequency of abnormally shaped somatic embryos without regeneration capacity. The structural organization of morphologically well-developed cotyledonary somatic embryos was similar to that of zygotic embryos. In abnormal somatic embryos the shoot apical meristem and root meristem were very damaged. Electrophoretic separation of denatured proteins using SDS-PAGE showed differences in the accumulation of low molecular weight storage proteins in somatic embryos. Storage protein accumulation was dependent on the concentration of PEG-4000 and the carbohydrate source.

Słowa kluczowe

EN

carbohydrate; polyethylene glycol; somatic embryo; embryo; maturation; hybrid; fir; Abies alba x Abies numidica; embryogenesis

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2004
• Tom: 46
• Opis fizyczny: p.159-167,fig.,ref.

Twórcy

autor

Salaj T

• Slovak Academy of Sciences, Akademicka 2, P.O.Box 39A, 950 07 Nitra 1, Slovak Republic

autor

Matusova R.

autor

Salaj J.

Bibliografia

• Attree SM, Moore D, Sawhney VK, and Fowke LC. 1991. Enhanced maturation and desiccation tolerance of white spruce [Picea glauca (Moench) Voss] somatic embryos: Effects of a non-plasmolysing water stress and abscisic acid. Annals of Botany 68: 519-525.
• Attree SM, and Fowke LC. 1993. Embryology of Gymnosperms: Advances in synthetic seed technology of conifers. Plant Cell, Tissue and Organ Culture 35: 1-35.
• Blanc G, Michaux-Ferriere N, Teisson C, Lardet L, and Carron MP. 1999. Effects of carbohydrate addition on the induction of somatic embryogenesis in Hevea brasiliensis. Plant Cell Tissue and Organ Culture 59: 103-112.
• Blanc G, Lardet L, Martin A, Jacob JL, and Carron MP. 2002. Differential carbohydrate metabolism conducts morphogenesis in embryogenic callus of Hevea brasiliensis (Müll. Arg.). Journal of Experimental Botany 53: 1453-1462.
• Bozkhov P, and von Arnold S. 1998. Polyethylene glycol promotes maturation but inhibits further development of Picea abies somatic embryos. Physiologia Plantarum 104: 211-224.
• Bradford MM. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry 72: 248-254.
• Canhoto JM, and Cruz GS. 1994. Improvement of somatic embryogenesis in Feioja sellowiana Berg. (Myrtaceae) by manipulation of culture media composition. In Vitro Cellular and Developmental Biology - Plant 30: 21-25.
• Erdelský K, and Frič F. 1979. Praktikum a analytické metódy vo fyziológii rastlin. SPN, Bratislava 621.
• Find J, 1997. Changes in endogenous ABA levels in developing somatic embryos of Norway spruce [Picea abies (L.) Karst.] in relation to maturation medium, desiccation and germination. Plant Science 128: 75-83.
• Finer JJ, Kriebel HB, and Becwar MR. 1989. Initiation of embryogenic callus and suspension cultures of eastern white pine (Pinus strobus L.). Plant Cell Reports 8: 203-206.
• Gajdošová A, Vooková B, Kormuták A, Libiaková G, and Doležel J. 1995. Induction, protein composition and DNA ploidy level of embryogenic calli of silver fir and its hybrids. Biologia Plantarum 37: 169-176.
• Gebhardt KH, Weisberger H, and Fröhlich HJ. 1988. In vitro germination and production of embryogenic callus from liquid suspension cultures of Abies alba. International Conifer Tissue Culture Working Group, poster No 17, Saskatoon, Canada.
• Grossnickle SC, and Major JE. 1994. Interior spruce seedlings compared with emblings produced from somatic embryogenesis. III. Physiological response and morphological development on a reforestation site. Canadian Journal of Forest Research 24: 1397-1407.
• Guevin TD, Micah V, and Kirby EG. 1994. Somatic embryogenesis in cultured mature embryos oiAbies balsamea. Plant Cell, Tissue and Organ Culture 37: 205-208.
• Gupta PK, and Durzan DJ. 1985. Shoot multiplication from mature trees of Douglas fir (Pseudotsuga menziesii) and sugar pine (Pinus lambertiana). Plant Cell Reports 4: 177-179.
• Hakman I. 1993. Embryology in Norway spruce (Picea abies). An analysis of seed storage proteins and adaptation of storage reserves during seed development and somatic embryogenesis. Physiologia Plantarum 87: 148-159.
• Häggman H, Jokela A, Krajnakova J, Kauppi A, Niemi K, and Aronen T. 1999. Somatic embryogenesis of Scots pine: cold treatment and characteristics of explants affecting induction. Journal of Experimental Botany 50: 1769-1778.
• Hristoforoglu K, Schmidt J, and Bolhar-Nordenkamp H. 1995. Development and germination of Abies alba somatic embryos. Plant Cell, Tissue and Organ Culture 40: 277-284.
• Hurkman WI, and Tanaka CK. 1986. Solubilization of plant membrane proteins for analysis by two-dimensional gel electrophoresis. Plant Physiology 81: 802-806.
• Iraqi D, and Tremblay FM. 2001. The role of sucrose during maturation of black spruce (Picea mariana) and white spruce (Picea glauca) somatic embryos. Physiologia Plantarum 111: 381-388.
• Jásik J, Salajová T, Kormuták A, and Salaj J. 1999. Somatic embryogenesis in hybrid firs. In: Jain SM, Gupta PK, Newton RJ [eds.], Somatic embryogenesis in woody plants, vol 4, 505-523. Kluwer Academic Publishers, Dordrecht.
• Jensen U, and Lixue C. 1991. Abies seed protein profile divergent from other Pinaceae. Taxon 40: 435-440.
• Klimaszewska K, and Smith DR. 1997. Maturation of somatic embryos of Pinus strobus is promoted by a high concentration of gellan gum. Physiologia Plantarum 100: 949-957.
• Kong L, Attree SM, Evans DE, Binarova P, Young EC, and Fowke LC. 1999. Somatic embryogenesis in white spruce: studies of embryo development and cell biology. In: Jain SM, Gupta PK, Newton RJ [eds.], Somatic embryogenesis in woody plants, vol. 3, 1-28. Kluwer Academic Publishers, Dordrecht.
• Krajňáková J, and Häggman H, 1997. Regeneration of Abies cephalonica through somatic embryogenesis. Identification and control of phase changes in rejuvenation. COST 822 - 4th meeting of WG3. Oct. 15-19, 102-104, Nitra, Slovakia.
• Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
• Leal I, Misra S, Attree SM, and Fowke LC. 1995. Effect of abscisic acid, osmoticum and desiccation on 11S storage protein gene expression in somatic embryos of white spruce. Plant Science 106: 121-128.
• Lelu A-M, Bastien C, Drugeault A, Gouez M-L, and Klimaszewska K. 1999. Somatic embryogenesis and plantlet development in Pinus sylvestris and Pinus pinaster on medium with and without growth regulators. Physiologia Plantarum 105: 719-728.
• Li XY, Huang FH, Murphy JB, and Gbur EE, 1998. Polyethylene glycol and maltose enhance somatic embryo maturation in loblolly pine (Pinus taeda L.). In Vitro Cellular and Developmental Biology - Plant 34: 22-26.
• Liao YK, and Amerson VH. 1995. Slash pine (Pinus elliottii Engelm) somatic embryogenesis. II. Maturation of somatic embryos and plant regeneration. New Forests 10: 165-182.
• Misra S, Attree SM, Leal I, and Fowke LC. 1993. Effect of abscisic acid, osmoticum, and desiccation on synthesis of storage proteins during the development of white spruce somatic embryos. Annals of Botany 71: 11-22.
• Norgaard JV, 1997. Somatic embryo maturation and plantlet regeneration in Abies nordmanniana Lk. Plant Science 124: 211-221.
• Norgaard JV, and Krogstrup P. 1991. Cytokinin-induced somatic embryogenesis from immature embryos of Abies nordmanniana Lk. Plant Cell Reports 9: 509-513.
• Ramarosandratana A, Harvengt L, Bouvet A, Calvayrac R, and Paques M. 2001. Influence of the embryonal suspensor mass (ESM) sampling on development and proliferation of maritime pine somatic embryos. Plant Science 160: 473-479.
• Salajová T, Jásik J, Kormuták A, Salaj J, and Hakman I. 1996. Embryogenic culture initiation and somatic embryo development in hybrid firs (Abies alba x Abies cephalonica and Abies alba x Abies numidica). Plant Cell Reports 15: 527-530.
• Salajová T, Salaj J, and Kormuták A. 1999. Initiation of embryogenic tissues and plantlet regeneration from somatic embryos of Pinus nigra Arn. Plant Science 145: 33-40.
• Schuller A, Reuther G, and Geier T. 1989. Somatic embryogenesis from seed explants of Abies alba. Plant Cell, Tissue and Organ Culture 17: 53-58.
• Sreedhar L, and Bewley JD. 1998. Nitrogen and sulfur-containing compounds enhance the synthesis of storage reserves in developing somatic embryos of alfalfa (Medicago sativa L.). Plant Science 134: 31-44.
• Stasolla C, Van Zyl L, Egertsdotter U, Craig D, Liu W, and Sederoff RR. 2003. The effect of polyethylene glycol on gene expression of developing white spruce somatic embryos. Plant Physiology 131: 49-60.
• Svobodová H, Albrechtová J, Kumstýřová L, Lipavská H, Vágner M, and Vondráková Z. 1999. Somatic embryogenesis in Norway spruce: Anatomical study of embryo development and influence of polyethylene glycol on maturation process. Plant Physiology and Biochemistry 37: 209-221.
• Tremblay L, and Tremblay FM. 1991. Carbohydrate requirements for the development of black spruce [Picea mariana (Mill.) B.S.P] and red spruce (P. rubens Sarg.) somatic embryos. Plant Cell, Tissue and Organ Culture 27: 95-103.
• Tremblay L, Levasseur C, and Tremblay F. 1999. Frequency of somaclonal variation in plants of black spruce (Picea mariana, Pinaceae) and white spruce (P. glauca, Pinaceae) derived from somatic embryogenesis and identification of some factors involved in genetic instability. American Journal of Botany 86: 1317-1381.
• Vooková B, Gajdošová A, and Matúšová R. 1998. Somatic embryogenesis in Abies alba x Abies alba and Abies alba x Abies nordmanniana hybrids. Biologia Plantarum 40: 523-530.
• Vooková B, and Kormuták A. 2001. Effect of sucrose concentration, charcoal, and indole-3-butyric acid on germination of Abies numidica somatic embryos. Biologia Plantarum 44: 181-184.