Hamujace dzialanie dekstranu na adhezje Pseudomonas aeruginosa do komorek nablonka policzka

• Autorzy: Wolska K. , Obrepalska M. , Jakubczak A.

Warianty tytułu

EN

Inhibition of adhesion to buccal epithelial cells of Pseudomonas aeruginosa by dextran

• Języki publikacji: PL

Abstrakty

PL

W pracy badano hamujący wpływ dekstranu na adhezję szczepów Pseudomonas aeruginosa do komórek nabłonka policzka in vitro. Dla stwierdzenia, czy hamujące działanie dekstranu było swoiste, czy też nie, dokonano również oceny innych neutralnych polisacharydów, tj. mannanu i glikogenu.

EN

Adhesion of Pseudomonas aeruginosa strains to buccal epithelial cells appears to be a necessary precondition for colonization and infection of respiratory tract. There are many strategies to prevent host organisms for Pseudomonas aeruginosa. The purpose of these studies was to evaluate the potential for preventing adhesion of Pseudomonas aeruginosa to epithelial cells with dextran. Dextran (5,000 MW) inhibited adhesion of Pseudomonas aeruginosa to buccal cells, at 20 mM was most inhibitory. The inhibitory effect appeared to be nonspecific because other neutral polysaccharides (glycogen and mannan) were also inhibitory. Dextran is an inexpansive and nontoxic agent and may be useful to prevent colonization and infection of respiratory tract with Pseudomonas aeruginosa.

Słowa kluczowe

PL

czlowiek; uklad oddechowy; mikroorganizmy; Pseudomonas aeruginosa; szczepy bakteryjne; adhezja bakterii; twarz; policzki; komorki nablonka; dekstran; mannany; glikogen; dzialanie hamujace; blona sluzowa policzka

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2005
• Tom: 57
• Numer: 3
• Opis fizyczny: s.311-318,rys.,bibliogr.

Twórcy

autor

Wolska K.

• Akademia Podlaska w Siedlcach, ul.B.Prusa 12, 08-110 Siedlce

autor

Obrepalska M.

autor

Jakubczak A.

Bibliografia

• 1. Aronson M, Medalia O, Schorl L i inni. Prevention of colonization of the urinary tract of mice with Escherichia coli by blocking of bacterial adherence with methyl alpha-D-mannopyranoside. J Infect 1979; 139: 329-32.
• 2. Azghani A O, Wiliams I, Holiday D В, Johanson A R. A beta-linked mannan inhibits adherence of Pseudomonas aeruginosa to human lung epithelial cells. Glycobiol 1995; 5: 39-44.
• 3. Barghouthi S, Guerdoud L M, Speert D P. Inhibition by dextran of Pseudomonas aeruginosa adherence to epithelial cells. Am J Respir Crit Care Med 1996; 154: 1788-93.
• 4. Bentzman S de, Roger P, Dupuit F i inni. Asialo GM1 is a receptor for Pseudomonas aeruginosa adherence to regenerating respiratory epithelial cells. Infect Immun 1996; 64: 1582-8.
• 5. Cacalano G, Kays M, Saiman L, Prince A. Production of the Pseudomonas aeruginosa neuraminidase is under hyperosmolar conditions and is regulated by genes involved in alginate expression. J Clin Invest 1992; 89: 1866-74.
• 6. Cronberg S, Robertson B, Nilsson I M, Nilehn J E. Suppresive effect of dextran on platelet adhesiveness. Thromb Diath Hemorrh 1966; 16: 384-94.
• 7. Davies J, Dewar A, Bush A i inni. Reduction in the adherence of Pseudomonas aeruginosa to native cystic fibrosis epithelium with anti-asialoGMl antibody and neuraminidase inhibition. Eur Respir J 1999; 13: 565-70.
• 8. Derrick J R, Guest M M. Dextrans: current concepts of basic actions and clinical applications. Charles C. Thomas, Springfield, IL, 1971.
• 9. Doig P Smith N R, Todd T, Irvin R T. Characterization of the binding of Pseudomonas aeruginosa alginate to human buccal epithelial cells. Infect Immun 1987; 55: 1517-22.
• 10. Doig P, Todd T, Sastry P A, Lee K K i inni. Role of pili in adhesion of Pseudomonas aeruginosa to human respiratory epithelial cells. Infect Immun 1988; 56: 1641-6.
• 11. Johanson W G, Woods D E, Chadhuri T. Association of respiratory tract colonization with adherence of gram-negative bacilli to epithelial cells. J Infect Dis 1979; 139: 667-73.
• 12. Kabat E, Вezer A E. The effect of variation in molecular weight on the antigenicity of dextran in man. Arch Biochem Biophys 1958; 78: 306-18.
• 13. Ljungstrom K.G. Safety of dextran in relation to other colloids-ten years experience with hapten inhibition. Infusionsth Transfusionsmed 1993; 20: 206-10.
• 14. McEachran D W, Irvin R T. Adhesion of Pseudomonas aeruginosa to human buccal cells epithelial cells: evidence for two classes of receptors. Can J Microbiol 1985; 31: 563-9.
• 15. Ramphal R, Pier G B. Role of Pseudomonas aeruginosa mucoid exopolysaccharide in adherence to tracheal cells. Infect Immun 1985; 47: 1-4.
• 16. Saiman L, Cacalano G, Gruenert D, Prince A. Comparison of adherence of Pseudomonas aeruginosa to respiratory epithelial cells from cystic fibrosis patients and healthy subjects. Infect Immun 1992; 60: 2808-14.
• 17. Sato H, Okinaga K. Role of pili in the adherence of Pseudomonas aeruginosa to mouse epidermal cells. Infect Immun 1987; 55: 1774-8.
• 18. Tang H В, Speert D P, Feldman M i inni. 6000 MW dextran reduces rate of acute Pseudomonas aeruginosa pneumonia in mouse model (abstract). Pediatr Pulmonol 1995; 12: 240-1.
• 19. Wolska K, Jakubczak A, Bukowski K. Pathogenicity of Pseudomonas aeruginosa. Adv Agric Sci 2002; 8: 27-38.
• 20. Wolska. K, Jakubczak A. Właściwości hemaglutynacyjne szczepów Pseudomonas aeruginosa. Medycyna Wet 2004; 60: 653-7.
• 21. Woods D E, Straus D C, Johanson WG, Jr, Berry V K, Bass J A. Role of pili in adherence of Pseudomonas aeruginosa to mammalian buccal epithelial cells. Infect Immun 1980; 29: 1146-51.
• 22. Woods D E, Bass J E, Johanson W G, Jr, Straus D C. Role of adherence in the pathogenesis of Pseudomonas aeruginosa lung infection in cystic fibrosis patients. Infect Immun 1980; 30: 694-9.