Neuralna regulacja uwalniania gonadoliberyny u owcy w roznych stanach fizjologicznych; rola receptorow GABA-A

• Autorzy: Tomaszewska-Zaremba D , Mateusiak K. , Przekop F.

Warianty tytułu

EN

The neural regulation of gonadoliberin release in ewes during different physiological stages: the role of GABA-A receptors

• Języki publikacji: PL

Abstrakty

EN

One of the most important inhibitory neurotransmitter in the control of gonadoliberin (GnRH) secretion is gamma-aminobutyric acid (GABA). To examine the role of GABAA receptor mediating systems in the control of GnRH/LH release from the preoptic area and ventromedial hypothalamus of ewes in different reproductive stages (seasonal anestrus, follicular and luteal phase of the estrous cycle) the extracellular concentration of GnRH, ß-endorphin, noradrenaline, dopamine and metabolites of catecholamines: MHPG, DOPAC were quantified during local stimulation or blockade of GABAA receptors with muscimol or bicuculline, respectively. Stimulation of GABAA receptors in the preoptic area and ventromedial hypothalamus markedly attenuatted GnRH/LH release in the anestrous ewes and in sheep during follicular phase of the estrous cycle but did not affect these hormones secretion in animals during the luteal phase of the estrous cycle. The activation of GABAA receptors in these structures in different way affected ß-endorphinergic and catecholaininergic system activity in anestrous ewes arid animals during the follicular phase; lack of changes in the release of ß-endorphin, noradrenaline and dopamine was noted in ewes during the luteal phase. With the exception of preoptic area in anestrous ewes the blockade of GABAA receptors in the preoptic and ventromedial hypothalamus affected in specifical way the activity of ß-endorphinergic and catecholaminergic systems in these structure in all animals. In conclusion: presented results indicate that activation of GABAA receptors in the preoptic area and ventromedial hypothalamus of anestrous ewes and sheep during follicular phase of the estrous cycle inhibits GnRH/LH secretion. Presented results indicate that different neural mechanisms may be involved in the suppression of GnRH/LH by GABA directly through the GABAA receptor mechanism on the perikaria or on the axon terminals of GnRH cells, and/or indirectly through GABAA receptor processes on ß-endorphinergic and catecholaminergic systems. Lack of changes in GnRH/LH release during GABAA receptor activation in ewes during luteal phase of the estrous cycle indicates that the influence of GABA on GnRH-ergic activity depends on physiological state of animals. Blockade of GABAA receptors in the anestrous and cycling ewes does not change GnRH/LH release; it is suggested that blockade of GABAA recreceptors may change GnRH neurons response to ß-endorphin and dopamine.

Słowa kluczowe

PL

noradrenalina; receptory GABA-A; hormony; rozrod; owulacja; neurofizjologia; cykl plciowy; dopamina; fizjologia; gonadoliberyny; neuroprzekazniki; owce; podwzgorze; regulacja

• Wydawca: -
• Czasopismo: Postępy Nauk Rolniczych
• Rocznik: 2005
• Tom: 52
• Numer: 5
• Opis fizyczny: s.7-16,tab.,bibliogr.

Twórcy

autor

Tomaszewska-Zaremba D

• Instytut Fizjologii i Zywienia Zwierzat im. J. Kielanowskiego PAN, ul.Instytucka 3, 05-110 Jablonna

autor

Mateusiak K.

autor

Przekop F.

Bibliografia

• [1] Bourguignon J.P., Gerard A., Purnelle G., Czajkowski V., Yamamoto C., Lemaitre M., Rigo J.M., Moonen G., Franchimont P. 1997. Duality of glutamatergic and GABAergic control of pulsatile GnRH secretion by hypothalamic explants. I. Effects of antisense oligodeoxynucleotides using explants including or excluding the preoptic area. J. Neuroendocrinology 9: 183-191.
• [2] Bowery N. 1989. GABAβ receptors and their significance in mammalian pharmacology. Trends Pharmacol. Sci. 10: 401-411.
• [3] Brooks A.N., Haynes N.B., Yung K. 1986. Ovarian steroids involvement in endogenous opioid modulation of LH secretion in seasonally anestrous mature ewes. J. Reprod. Fert. 76: 709-715.
• [4] Conover C.D., Kuljis S.O., Rabii J., Advis J.P. 1993. ß-endorfin regulation of luteinizing hormone-releasing hormone release at median eminence in ewes: immunocytochemical and physiological evidence. Neuroendocrinology 57: 1182-1195.
• [5] Curlevis J.D., Naylor A.M., Rhind S.M., Mc Neilly A.S. 1991. Effect of beta-endorphin on pulsatile luteinizing hormone and prolactin secretion during the follicular phase in the ewe. J. Neuroendocrinology 3: 123-126.
• [6] Domański E., Chomicka L.K., Ostrowska A., Gajewska A., Mateusiak K. 1991. Release of luteinizing hormone-releasing hormone, ß-endorphin and norepinephrine by nucleus infundibularis-median eminence during periovulatory period in the sheep. Neuroendocrinology 54: 151-158.
• [7] Ebling F.J.P., Lincoln G.A. 1985. Endogenous opioids and the control of seasonal LH secretion in Soy rams. J. Endocrinology 107: 341-353.
• [8] Felder C., Jarry H., Leonhardt S., Wuttke W., Moguilevsky J.A. 1996. The GABAergic control of gonadotrophin-releasing hormone secretion in male rats during sexual maturation involves effects on hypothalamic excitatory and inhibitory amino acids systems. Neuroendocrinology 64: 305-312.
• [9] Ferreira S.A., Scott C.J., Kuehl D.E., Jackson G.L. 1996. Differential regulation of luteinizing hormone release by γ-aminobutyric acid receptor subtypes in the arcuate-ventromedial region of the castrated ram. Endocrinology 137: 3453-3460.
• [10] Goodman R.L. 1996. Neural systems mediating the negative feedback actions of estradiol and progesterone in the ewe. Acta Neurobiol. Exp. 56: 727-741.
• [11] Haies T.G., Sanderson M.J., Charles A.C. 1994. GABA has excitatory effect on GnRH-secreting immortalized hypothalamic (GT 1-7) neurons. Neuroendocrinology 59: 297-308.
• [12] Hartman R.D., He J-R., Barraclough C.A. 1990. γ-aminobutyric acid-A and B receptor antagonists increases luteinizing hormone-releasing hormone neural responsiveness to intracerebroventricular norepinephrine in ovariectomized estrogen treated rats. Endocrinology 127: 1336-1345.
• [13] Havern R.L., Whisnant C.S., Goodman R.L. 1994. Dopaminergic structures in the ovine hypothalamus mediating estradiol negative feedback in anestrous ewes. Endocrinology 134: 1904-1914.
• [14] Jurry H., Leonhardt S., Wuttke W. 1991. γ-aminobutyric acid neurons in the preoptic/anterior hypothalamic area synchronize the phasic activity of the gonadotropin releasing hormone pulse generator in ovariectomized rats. Neuroendocrinology 53: 261-267.
• [15] Jung H., Shannon E.M., Fritschy J-M., Ojeda S.R. 1998. Several GABA receptor subunits are expressed in LHRH neurons of juvenile female rats. Brain Res. 790: 219-229.
• [16] Kaupmann K., Huggel K., Heid J. 1997. Expression cloning GABAß receptors uneovers similarity to metabotropic glutamate receptors. Nature 386: 239-246.
• [17] Li X.F., Bowe J.E., Mitchell J.C., Brain S.D., Lightman S.L., O'Byme K.T. 2004. Stress-induced suppression of the gonadotropin-releasing hormone pulse generator in the female rat: a novel neural action for calcitonin gene-related peptide. Endocrinology 145: 1556-1563.
• [18] Mitsushima D., Hei D.L., Tarasawa E. 1994. γ-aminobutyric acids is an inhibitory neurotransmitter restricting the release of luteinizing hormone-releasing hormone before the onset of puberty. Proc. Natl. Acad. Sci. USA 91: 395-399.
• [19] Mott D.D., Lewis D.W. 1994. The pharmacology and function of central GABAß receptors. Int. Rev. Neurobiol. 36: 97-223.
• [20] Nappi R.E., Rivest S. 1997. Effect of immune and metabolic challenge on the luteinizing hormone-releasing hormone neural system in cycling female rats: an evaluation at the transcriptional level. Endocrinology 138: 1374-1384.
• [21] Pacák K., Palkovits M.2001. Stressor specificity of central neuroendocrine responses implications for stress-related disorders. Endocr. Rev. 22: 502-548.
• [22] Petersen J.E., Mc Crone S., Coy D., Adelman J.P., Mahom L.C. 1993. GABAA receptor subunit mRNA in cells of preoptic area: colocalization with LHRH mRNA using dual-label in situ hybridization histochemistry. Endocr. J. 1: 29-34.
• [23] Przekop F., Wolińska-Witort E., Mateusiak K., Sadowski B., Domański E. 1984. The effect of prolonged stress on the estrous cycles and prolactin secretion in sheep. Anim. Reprod. Sci. 7: 333-342.
• [24] Przekop F., Hamam M.M., Mateusiak K. 1989. Lack of LH response to intraventricularly infused naloxone in anestrous ewes. Endocrinology of Farm Animals. Proceeding of II International Symposium on Farm Animal Endocrinology. Smolenice Castle: 255-260.
• [25] Rabow L.E., Russek S.J., Farb D.H. 1995. From ion currents to genomic analysis: recent advances in GABAA receptor research. Synapse 21: 189-274.
• [26] Rivier C., Rivest S. 1991. Effect of stress on activity of hypothalamic-pituitary-gonadal axis: peripheral and central mechanisms. Biol. Reprod. 45: 523-532.
• [27] Robinson J.E., Kendrick K.M. 1992. Inhibition of luteinizing hormone secretion in the ewe by progesterone: associated changes in the release of gamma-aminobutyric acid and noradrenaline in the preoptic area as measured by intracranial microdialysis. J. Neuroendocrinology 4: 231-236.
• [28] Robinson J.E. 1995. Gamma-aminobutyric acid and the control of GnRH secretion in sheep. J. Reprod. Fertil. Suppl 49: 221-230.
• [29] Robinson J.E., Healey A.E., Harris T.G., Messet E.A., Skinner D.C., Taylor J.A., Evans N.P. 2000. The negative action of progesterone on luteinizing hormone release is not associated with changes in GnRH mRNA expression in the ewe. J. Neuroendocrinology 12: 121-130.
• [30] Scott C.J., Clarke I.J. 1993. Inhibition of luteinizing hormone secretion in ovariectomized ewes during the breeding season by γ-aminobutyric acid (GABA) is mediated by GABAA receptors, but not GABAß receptors. Endocrinology 132: 1789-1796.
• [31] Scott C.J., Clarke I.J. 1993. Evidence that changes in the function of the subtypes of the receptors for gamma-aminobutyric acid may be involved in the seasonal changes in the negative feedbeck effects of estrogen on gonadotropin-releasing hormone secretion and plasma luternizing hormone levels m the ewe. Endocrinology 133: 2904-2912.
• [32] Sieghart W. 1995. Structure and pharmacology of gamma-aminobutyric acid A receptor subtypes. Pharmacol. Rev. 47: 181-234.
• [33] Sin J.A., Skinner M.J., Pape J.R., Herbison A.E. 2000. Late postnatal reorganization of GABA receptor signalling in native GABA neurons. Eur. J. Neurosci. 12: 3497-3504.
• [34] Skinner D.C., Herbison A.E. 1997. Effect of photoperiod onestrogen receptors, tyrosine hydroxylase, neuropeptide Y, and ß-endorphin immunoreactivity in the ewe hypothalamus. Endocrinology 138: 2585-2595.
• [35] Tanabe K., Nishijo H., Muragushi A., Ono T. 2000. Effects of chronic stress onhypothalamic interleukin-1B, interleukin-2, and gonadotropin-releasing hormone gene expression in ovariectomized rats. J. Neuroendocrinology 12: 13-21.
• [36] Tomaszewska D., Mateusiak K., Przekop F. 1999. Changes in extracellular LHRH and ß-endorphin-like immunoreactivity in the nucleus infundibularis-median eminence of anestrous ewes under stress condition. J. Neural. Trans. 106: 265-274.
• [37] Tomaszewska D., Przekop F. 1999. Catecholaminergic activity in the medial preoptic area and nucleus infundibularis-median eminence of anestrous ewes in normal physiological state and under stress condition. J. Neural. Trans. 106: 1031-1043.
• [38] Tomaszewska D., Przekop F., Mateusiak K. 2002. Responses of catecholaminergic, ß-endorphinergic and gonadoliberinergic neuronal systems in the hypothalamus of anestrous ewes to stressful stimuli. W: Stress: Neural, endocrine and molecular studies. R. McCarty, G. Aguilera, E. Sabban, R. Kvetnansky (red,). Taylor & Francis, London New York: 51-55.
• [39] Tomaszewska-Zaremba D., Mateusiak K., Przekop F. 2003. The role of GABAA receptors in the neural systems of the medial preoptic area in the control of GnRH release in ewes during follicular phase. Anim. Reprod. Sci. 77: 71-83.
• [40] Tomaszewska-Zaremba D., Mateusiak K., Przekop F. 2003. The role of GABAA receptors in the neural systems of the ventromedial hypothalamus-nucleus infundibularis region in the control of GnRH release in ewes during follicular phase. Exp. Clin. Endoerinol. Diab. 111: 335-340.
• [41] Tomaszewska-Zaremba D., Mateusiak K., Przekop F. 2002. The involvement of GABAA receptors in the control of GnRH and ß-endorphin release, and catecholaminergic activity in the preoptic area in anestrous ewes. Exp. Clin. Endocrinol. Diab. 110: 336-342.
• [42] Tomaszewska-Zaremba D., Mateusiak K., Przekop F. 2004. The role of GABAA receptors in the neural systems of the medial preoptic area in the control of GnRH release during the luteal phase of the estrous cycle in ewes. J. Anim. Feed Sci. 13: 123-134.
• [43] Tomaszewska-Zaremba D., Przekop F., Mateusiak K. 2001. The involvement of GABAA receptors in the control of GnRH and ß-endorphin release, and catecholaminergic activity in the ventromedial-infundibular region of hypothalamus in anestrous ewes. J. Physiol. Pharmacol. 52: 489-500.
• [44] Tomaszewska-Zaremba D., Mateusiak K., Przekop F. 2002. The role of GABAA receptors tn the neural systems of the ventromedial-infundibular region of the hypothalamus in the control of gonadotrophin release during luteal phase in ewes. J. Physiol. Pharmacol. 53: 1-11.
• [45] Tomaszewska-Zaremba D., Przekop F. 2005. Effects of GABAs receptor modulation on gonadotropin-releasing hormone and ß-endorphin release, and on catecholaminergic activity in the ventromedial hypothalamus-infundibular region of anestrous ewes. J. Neuroendocrinology 17: 49-56.
• [46] Tortonese D.J. 1999. Interaction between hypothalamic dopaminergic and opioidergic systems in the photoperiodic regulation of pulsatile luteinizing hormone secretion in sheep. Endocrinology 140: 750-757.
• [47] Wisden W., Seeburg P.H. 1992. GABAA receptor channels: from subunits to functional entities. Curr. Opin. Neurobiol. 2: 263-269.