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2010 | 66 | 01 |

Tytuł artykułu

Unieszkodliwianie kwasem cytrynowym paleczek Salmonella w podlozach mikrobiologicznych i tuszkach indyczych

Autorzy

Warianty tytułu

EN
Elimination of Salmonella spp. in bacteriological media and in turkey carcasses with citric acid

Języki publikacji

PL

Abstrakty

EN
The aim of this study was to determine the influence of citric acid in different concentrations on Salmonella spp. in microbiological media and in turkey carcasses. The influence of 0.01%, 0.02%, 0.03%, 0.05%, 0.1%, 0.25%, 0.5%, 1%, 1.5%, 2.0% citric acid concentrations in nutrient agar on Salmonella Enteritidis no. 33/66, Salmonella Anatum no. 30/93, Salmonella Typhimurium no. 227/84, was studied. Citric acid in 0.1% concentration agar media totally inhibits the growth of all Salmonella strains studied. At the concentration of 0.05% the bacteria count of S. Anatum, compared with the control, decreased by 2 logarithmic cycles and the count of S. Enteritidis, and S. Typhimurium by 1 logarithmic cycles only. Additional investigations were carried out on 150 turkey breast samples bought from poultry plants. The samples were contaminated with Salmonella Enteritidis no. 33/66. Afterwards, each sample was moved to 1%, and 2% citric acid solution for a period of 15 minutes. The results obtained after immersing turkey carcass elements in citric acid indicate that detecting Salmonella spp. in the samples was dependent on the inoculum of these bacteria on the poultry carcass surface. Salmonella spp. were not found when the surface of an element of a turkey carcass was contaminated with 10¹ Salmonella spp. colony-forming-units (cfu) and immersed for 15 minutes in 1% and 2% citric acid aqueous solution, while at a 10² cfu contamination, Salmonella spp. was detected in a smaller number of samples compared with the control samples. The inhibiting influence of citric acid on Salmonella spp. in bacterial substrates can also occur on poultry carcasses. The unfavourable action of this compound on Salmonella bacteria was stronger in bacterial media than in poultry carcasses.

Wydawca

-

Rocznik

Tom

66

Numer

01

Opis fizyczny

s.59-62,rys.,tab.,bibliogr.

Twórcy

  • Uniwersytet Warminsko-Mazurski w Olsztynie, ul.Warszawska 30, 10-082 Olsztyn

Bibliografia

  • 1.Breen P. J., Salari H., Compadre C. M.: Elimination of Salmonella contamination from poultry tissues by cetylpyridinium chloride solutions. J. Food Prot. 1997, 60, 1019-1021.
  • 2.Conner D. E., Bilgili S. F.: Skin attachment model for improved laboratory evaluation of potential carcass disinfectants for their efficacy against Salmonella attached to broiler skin. J. Food Prot. 1994, 57, 684-688.
  • 3.Dickson J. S.: Acetic acid action on beef tissue surfaces contaminated with Salmonella typhimurium. J. Food Sci. 1992, 57, 297-301.
  • 4.Dickson J. S., Anderson M. E.: Microbiological decontamination of food animal carcasses by washing and sanitizing systems: a review. J. Food Prot. 1992, 55, 133-140.
  • 5.Dickson J. S., Nettles Cutter C. G., Siragusa G. R.: Antimicrobial effects of trisodium phosphate against bacteria attached to beef tissue. J. Food Prot. 1994, 57, 952-955.
  • 6.Dorsa W. J., Cutter C. N., Siragusa G. R.: Effects of acetic acid, lactic acid and trisodium phosphate on the microflora of refrigerated beef carcass surface tissue inoculated with Escherichia coli O157:H7, Listeria innocua, and Clostridium sporogenes. J. Food Prot. 1997, 60, 619-624.
  • 7.ISO 6579: 1993(E). Microbiology - General guidance on methods for the detection of Salmonella. International Organization of Standardization, Geneva, Switzerland.
  • 8.Jetton J. P., Bilgili S. F., Conner D. E., Kotrola J. S., Reiber M. A.: Recovery of salmonellae from chilled carcasses as affected by rinse media and enumeration method. J. Food Prot. 1992, 55, 329-333.
  • 9.Kotula K., Thelappurate R.: Microbiological and sensory attributes of retail cuts of beef treated with acetic and lactic acid solutions. J. Food Prot. 1994, 57, 665-670.
  • 10.Lillard H. S.: Effect of trisodium phosphate on salmonellae attached to chicken skin. J. Food Prot. 1994, 57, 465-469.
  • 11.Mikołajczyk A., Radkowski M.: Elimination of Salmonella spp. by lactic acid. Pol. J. Vet. Sci. 2002, 5, 139-143.
  • 12.PN-ISO 6579 1998. Mikrobiologia. Ogólne zasady metod wykrywania pałeczek Salmonella.
  • 13.Radkowski M., Mikołajczyk A.: Wpływ chlorku heksadecylopirydyniowego na unieszkodliwianie pałeczek Salmonella w mięsie. Medycyna Wet. 2004, 60, 150-253.
  • 14.Rozporządzenie Ministra Zdrowia i Opieki społecznej z dnia 18 września 2008 r. w sprawie dozwolonych substancji dodatkowych - wraz z załącznikami. Dz. U. 2008, nr 177 poz. 1094, z dnia 3 października 2008 r.
  • 15.Russell S. M., Fletcher D. C., Walker J. M., Bailey J. S.: The effect of hydrogen peroxide and sodium bicarbonate rinses on the recovery of bacteria from broiler carcasses. Poult. Sci. 1993, 72 (supp. 1), 190-193.
  • 16.Sawaya W. N., Elnawawy A. S., Al-Zenki S., Al-Otaibi J., Al-Omirah I. I., Al-Amiri H.: Storage stability of chicken as affected by MAP and lactic acid treatment. J. Food Sci. 1995, 60, 611-614.
  • 17.Smulders F. J. M., Upmann M.: Verminderung der bakteriellen Belastung auf frischem Fleisch. Fleischwirtschaft 2000, 80, 27-29.
  • 18.Tamblyn K. C., Conner D. E.: Bactericidal activity of organic acids against Salmonella typhimurium attached to broiler chicken skin. J. Food Prot. 1997, 60, 629-633.
  • 19.Tsai Y. W., Ingham S. C.: Survival of Escherichia coli O157:H7 and Salmonella spp. in Acidic condiments. J. Food Prot. 1997, 60, 751-755.
  • 20.Wang W.-Ch., Li Y., Slavik M., Xiong H.: Trisodium phosphate and cetylpyridinium chloride spraying on chicken skin to reduce attached Salmonella typhimurium. J. Food Prot. 1997, 60, 992-994.
  • 21.Xiong H., Li Y., Slavik M. F., Walker J. T.: Spraying chicken skin with selected chemicals to reduce attached Salmonella typhimurium. J. Food Prot. 1998, 61 , 272-275.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-874b3fe6-5994-4cc6-a017-757bb35fb23e
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