Application of meat juice in diagnosis of brucellosis in hares and wild boars by ELISA

• Autorzy: Szulowski K , Pilaszek J. , Iwaniak W.
• Języki publikacji: EN

Abstrakty

EN

Meat juice and serum samples from hares and wild boars were used in the ELISA for the examination for the presence of anti-Brucella antibodies. A high correlation between meat juice ELISA and serum ELISA was obtained. All 9 meat juice samples from hares with anatomopathological lesions characteristic of brucellosis (from 8 animals Brucella was isolated) were positive in the ELISA, similarly as sera. Out of 46 remaining hares only 1 reacted positively in the meat juice ELISA and it was not confirmed by the serum ELISA and other methods. As concerns the material from wild boars 12.3% of sera (out of 933 samples) and 13.7% of meat juice samples ( out of 336) were positive in the ELISA. Among 48 serum and meat juice samples coming from the same animals, 6 samples were positive both in the serum ELISA and meat juice ELISA and 42 were negative. The meat juice ELISA proved to be a valuable method for testing both hares and wild boars for anti-Brucella antibodies.

Słowa kluczowe

EN

meat juice; serology; wild boar; diagnosis; brucellosis; ELISA test; hare

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2000
• Tom: 44
• Numer: 1
• Opis fizyczny: p.45-52,fig.

Twórcy

autor

Szulowski K

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Pilaszek J.

autor

Iwaniak W.

Bibliografia

• 1. Alton G.G., Jones L.M., Angus R.D., Verger J.M.: Techniques for the Brucellosis Laboratory. INRA, Paris, France, 1988.
• 2. Alton G.G.: Brucella suis. In: Animal Brucellosis, Eds. Nielsen K., Duncan J.R., CRC Press, Boca Raton, Florida, pp. 411-423, 1990.
• 3. Cook D.R., Noble J.W.: Isolation of Brucella suis from cattle. Aust. Vet. J. 61, 263, 1984.
• 4. Davis D.S.: Brucellosis in wildlife. In: Animal Brucellosis, Eds. Nielsen K., Duncan JR., CRC Press, Boca Raton, Florida, pp. 321-334, 1990.
• 5. Fenske G.: Untersuchungen über Bruzellose bei Hasen. Mh. Vet. Med. 18, 380, 1963.
• 6. Godfroid J., Michel P., Uytterhaegen L., De Smedt C., Rasseneur F., Boelaert F., Saegerman C., Patigny X.: Brucellose enzootique a Brucella suis biotype 2 chez le sanglier (Sus scrofa) en Belgique. Ann. Med. Vet. 138, 263, 1994.
• 7. Heck F.C., Williams J.D., Pruett J., Sanders R., Zink D.L.: Enzyme-linked immunosorbant assay for detecting antibodies to Brucella abortus in bovine milk and serum. Am. J. Vet. Res. 41, 2082, 1980.
• 8. Instruction no 52 of the Ministry of Agriculture, Warsaw, 1980.
• 9. Instruction no 55 of the Ministry of Agriculture, Warsaw, 1984.
• 10. Instruction no 2/96 of the Ministry of Agriculture, Warsaw, 1996.
• 11. Joint FAO/WHO Expert Comm. Brucellosis, Sixth Report WHO, Geneva, 1986.
• 12. Manual of Standards for Diagnostic Tests and Vaccines for Lists A and B Diseases of Mammals, Birds and Bees, OIE, Paris, France 1996.
• 13. Pilaszek J., Szulowski K., Truszczyński M.: Bruceloza zajęcy i jej diagnostyka. Życie Wet. 71, 180, 1996.
• 14. Szulowski K., Pilaszek J., Truszczyński M.: Zestaw ELISA do badania surowic świń w kierunku brucelozy. Medycyna Wet. 52, 513, 1996.
• 15. Szulowski K., Iwaniak W., Pilaszek J., Truszczyński M., Chrobocińska M.: The ELISA for the examination of hare sera for anti-Brucella antibodies. Comp. Immun. Microbiol. Infect. Dis. 22, 33, 1999.
• 16. Szulowski K.: Evaluation of the ELISA in diagnosis of bovine brucellosis. Part II - examination of milk. Polish. J. Vet. Sci. 2, 59, 1999.
• 17. Szulowski K.: Diagnosis of Brucella suis infections in pigs and hares by ELISA. Polish. J. Vet. Sci. 2, 65, 1999.
• 18. Szulowski K., Pilaszek J., Iwaniak W.: Wstępne badania nad występowaniem bruclozy u dzików. Życie Wet. 74, 399, 1999.