Oligodeoxyadenylate stimulates the protein kinase activity of anti-DNA sIgA from human milk

• Autorzy: Kit Y. , Kuligina E. , Semenov D. , Richter V.
• Języki publikacji: EN

Abstrakty

EN

Preparations of anti-DNA sIgA were obtained from human milk by sequential chro­matography on protein A-sepharose, DEAE-fractogel and DNA-cellulose. The influ­ence of oligonucleotides on protein kinase activity was investigated. It was discovered that incubation of anti-DNA sIgA with oligodeoxyriboadenylate d(A)12 stimulates the phosphorylation of polypeptides of sIgA in the presence of [γ- 32P]ATP. The greatest was the incorporation of 32P into the sIgA H-chains. We also demonstrated stimula­tion of the casein kinase activity of anti-DNA sIgA by d(A)12. The stimulation of the protein kinase activity of anti-DNA sIgA by oligoriboadenylate r(A)12 was not de­tected.

Słowa kluczowe

EN

anti-DNA antibody; human milk; protein kinase activity; milk; oligonucleotide; protein kinase; antibody

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2002
• Tom: 49
• Numer: 1
• Opis fizyczny: p.291-294,fig.

Twórcy

autor

Kit Y.

• National Academy of Sciences of Ukraine, Dragomanov 14-16, 79005, Lviv, Ukraine

autor

Kuligina E.

autor

Semenov D.

autor

Richter V.

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