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1998 | 44 | 4 |

Tytuł artykułu

Inkubacja doroslych osobnikow Ascaris suum w mediach chemicznie zdefiniowanych

Treść / Zawartość

Warianty tytułu

Języki publikacji

PL

Abstrakty

EN
Adult Ascaris suum were incubated at 37°C in Ringer solution for Ascaris (ARSb) and in ARSb with 0.2 % addition of glucose, maltose, trehalose, saccharose, starch and glycogen. Control worms were incubated in ARSb without natrium acetate (ARS0). The survival rate, motility and elasticity of each individual, changes of the body weight and glycogen contents in muscles were determined. The incubation in ARS0 was finished on the 7th day. The longest (11 days) lived the worms kept in the basic medium ARSb and ARSb with maltose. Regarding their influence on the survival rate of parasites, the rest sugars could be set in order as follows: glycogen and glucose (10 days), trehalose, saccharose and starch (9 days). In the media containing saccharose and starch worms showed reduced motility and elasticity. On the 7th day of incubation the glycogen level in muscles of control worms was reduced to 0.75% of its beginning concentration, whereas in muscles of Ascaris from media with glucose and trehalose over 50% and with maltose 43% of polysaccharide's reserves were preserved. A slight increase (10-15%) in average body weight of worms incubated with glucose, maltose and glycogen was observed, only in the control and medium with saccharose changes of worms' mass were significant, amounting to 30% and 80%, respectively.

Wydawca

-

Rocznik

Tom

44

Numer

4

Opis fizyczny

s.671-679, rys.,tab.,bibliogr.

Twórcy

autor
  • Wyzsza Szkola Pedagogiczna, Zolnierska 14, 10-561 Olsztyn
autor
autor

Bibliografia

  • BEHM C. A. 1997. The role of trehalose in physiology of nematodes. Inter. J. Parasitol. 27: 215-229.
  • BRAND VON T. 1973. Biohemistry of parasites. Sec. ed, Acad. Press, N.Y. - London.
  • DONAHUE M. J., YACOUB N. J., KAEIN M. R., MASARACCHIA R. A., HARRIS B. G. 1981. Glycogen metabolizing enzymes during starvation and feeding of Ascaris suum maintained in a perfusion chamber. J. Parasitol. 67: 505-510.
  • DOUVRES F. W., URBAN J. F. JR. 1986. Development of Ascaris suum from in vivo-derived third-stage larvae to egg-laying adults in vitro. Proc. Helminthol. Soc. Wash. 53: 256-262.
  • - - 1987. Nematoda except parasite of insects. In: A. E. R. TAYLOR, J. R. BAKER [Eds.] In vitro methods for parasite cultivation, Acad. Press, London-Toronto: 318-378.
  • FETTERER R. H. 1986. Transcuticular solute movement in parasite nematodes: Relationship between non-polar solute transport and partition coefficient. Comp. Biochem. Physiol. 84A: 461-466.
  • FLEMING M. W. 1998. In vitro growth of swine roundworm larvae, Ascaris suum: Cultivation techniques and endocrine regulation. J. Helminthol. Soc. Wash. 63: 69-73.
  • - FETIERER R. H. 1984. Ascaris suum: Continous perfusion of the pseudocoelom and nutritient absorption. Exp. Parasitol. 97: 142-148.
  • GRABDA J. 1976. Studies on the life cycle and morphogenesis of Anisakis simplex (Rudolphi, 1809) (Nematoda: Anisakidae) cultured in vitro. Acta Ichthyol. Pisc. 6: 119-141.
  • HO N. F. H., GEARY T. G., BARSUHN C. L., SIMS S. M., THOMPSON D. P. 1992. Mechanistic studies in the transcuticular delivery of antiparasitic drugs. II- ex vivo/in vitro correlation of solute transport by Ascaris suum. Mol. Biochem. Parasitol. 52: 1-14.
  • - - RAUB T. J., BARSHUN C. L., THOMPSON D. P. 1990. Biophysical transport properties of cuticule of Ascaris suum. Ibid. 41: 153-166.
  • IGLESIAS L., VALERO A., ADROHER F. J. 1997. Some factors which influence the in vitro maintenance of Anisakis simplex (Nematoda). Folia Parasitol. 44: 297-301.
  • JABŁONOWSKI Z., PIECHOCKI D. 1988. Metody hodowli pasożytniczych nicieni in vitro. Wiad. Parazytol. 34: 93-111.
  • KARANU F. N., RURANGIRWA F. R., MCGUIRE T. C., JASMER D. P. 1993. Haemonchus contortus: Identification of proteases with diverse characteristics in adut worm excretory-secretory products. Exp. Parasitol. 77: 362-372.
  • LU N. C., GOETSCH K. M. 1993. Carbohydrate requirement of Caenorhabditis elegans and the final development in chemically defined medium. Nematologica 39: 303-311.
  • MARTIN R. J., KUSEL J. R., ROBERTSON S. J., MINTA A., HAUGLAND R. P. 1992. Distribution of a fluorescent ivermectin probe, bodipy ivermectin, in tissues of the nematode parasite Ascaris suum. Parasitol. Res. 78: 341-348.
  • MAYES P. A. 1995. Metabolizm glikogenu. W: R. K. MURRAY, D. K. GRANNER, P. A. MA YES, V. W. RODWELL. Biochemia Harpera, wyd. 3, PZWL, Warszawa: 217-225.
  • PAX R. A., GEARY T. G., BENNETT J. L., THOMPSON D. P. 1995. Ascaris suum; characterization of transmural and hypodermal potentials. Exp. Parasitol. 80: 85-97.
  • RIUX A., KOMUNIECKI R. 1983. Methylvalerate formation in mitochondria of Ascaris suum and its relationship to anaerobic energy generation. J. Comp. Biochem. Physiol. 154: 439-354.
  • SØLLING H., ESMANN V. 1975. A sensitive method of glycogen determination in the presence of interfering substances utilizing the filter-paper technique. Anal. Biochem. 68: 664-668.
  • ŻÓŁTOWSKA K. 1995. Amylazy w układzie pasożyt-żywiciel. WSP, Olsztyn.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-86d999e5-0da8-493f-a087-9dbc86c28549
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