Wykorzystanie testu ELISA do rozpoznawania niektorych chorob zakaznych bydla przy uzyciu prob mleka

• Autorzy: Klimentowski S , Rypula K. , Zieminski R. , Folwarczny J. , Rulka J.
• Języki publikacji: PL

Abstrakty

EN

The aim of the investigations was the development of a milking system for the purpose of its use for control examinations of EEB, brucellosis and IBR using ELISA. The examinations were performed on 18 chosen farms and in 309 individual farms free of brucellosis and generally free of BLV and BHV-1. A total of 2250 milking cows from 4 voivodships in the south-west of Poland were examined. The blood was examined consecutively with by ELISA milk test for EBB by AGID and for brucellosis in the OKAP test. In the case of IBR SN was used only in the cows positive in ELISA milk test. Positive results in the diagnosis of EBB infection were more often noted in the ELISA milk test (30 and 28 cases) than in AGID test using blood sera (17 and 17 cases). Both ELISA milk test and the classic serological test used to diagnose brucellosis were negative. In the examinations for IBR, in all but one case, all positive ELISA milk test results were confirmed by the SN test with blood sera. It is worth noting the presence in all tests of significant differences in color intensity both in positive and negative samples which indicates a high sensitivity and specificity of the tests.

Słowa kluczowe

PL

bydlo; zapalenie nosa i tchawicy bydla; przeciwciala; choroby zakazne; probki mleka; diagnostyka; bruceloza; choroby zwierzat; enzootyczna bialaczka bydla; test ELISA

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 1995
• Tom: 51
• Numer: 02
• Opis fizyczny: s.86-90,rys.,tab.,bibliogr.

Twórcy

autor

Klimentowski S

• Akademia Rolnicza, Pl.Grunwaldzki 45, 50-366 Wroclaw

autor

Rypula K.

autor

Zieminski R.

autor

Folwarczny J.

autor

Rulka J.

Bibliografia

• 1. Bause J.: Schmidt F. W.: Tierarztl. Umsch. 35, 642, 1980.
• 2. Behrens F., Ziegelmeier R., Toth T., Keyserlingk M., Forschner E.: Beri. Munch, tierarztl. Wschr. 92, 527, 1979.
• 3. BottcherJ., Gottschalk E., Greiserwilke J., Moenning V., Bommeli W.: Rev. O IE 12, 461, 1993.
• 4. Eloit M., Vuiellaume A., Duřet C., Prevost P., Toma B.: Reel. Med. Vet. 163, 555, 1987.
• 5. Forschner E., Búnger Dt. tierarztl. Wschr. 93, 112, 1986.
• 6. Forschner E., Búnger Dt. tierarztl. Wschr. 93, 269, 1986.
• 7. Forschner E., Búnger I.: Dt. tierarztl. Wschr. 93, 328, 1986.
• 8. Forschner E., Búnger J., Krause H. P., Kútler DDt. tierarztl. Wschr. 96. 473, 1989.
• 9. Forschner E., Lehmacher W.: Dt. tierarztl. Wschr. 99, 87, 1992.
• 10. Hof-Jorgensen R.: Vet. Immun. Immunpath. 22, 293, 1989.
• 11. Hof-Jorgensen R.: OIE Manual. T. 3, 1991.
• 12. Keyserlingk M.: Untersuchungen zum Verhalten matemaler BLV-Antikórper im Blutserum von Kalbern und ihre Bedeutung in tierseuchenrechtlichen sicht. Praca dokt. Hanower, 1980.
• 13. KnoselH., Hempel E., Forschner E.: Dt. tierarztl. Wschr. 98, 325, 1991.
• 14. MammerickxM., Portetelle D., Bumy A.: Zbl. Vet. Med. В 32, 526, 1985.
• 15. Moenning V., Plagemann P. G. W.: Adv. Virus Res. 41, 53, 1992.
• 16. Niskanen R.: Vet. Rec. 133, 341, 1993.
• 17. Pallesen U.: Dt. tierarztl. Wschr. 93, 319, 1986.
• 18. Straub O. C., Fischer C. W., Frerking H.: Tierarztl. Umsch. 33,657, 1978.
• 19. Council Directive 88/406/EEC: Official Journal EC No L 194/1.
• 20. Council Directive 91/499/ECC: Official Journal EC No L 268/107.