Reconstitution of UV-damaged DNA into chromatin using Xenopus oocyte extracts

• Autorzy: Widlak P.
• Języki publikacji: EN

Abstrakty

EN

Chromatin was reconstituted in vitro using Xenopus oocyte extracts and plasmid DNA containing UV radiation-induced damage. Damaged DNA was assembled into minichromosomes with an efficiency similar to that of control, non-irradiated DNA. Oocyte extracts were competent to carry out DNA repair, which was elicited by nicking damaged templates followed by DNA synthesis during chromatin assembly. Newly synthesized DNA was efficiently reconstituted into nucleosomes.

Słowa kluczowe

EN

chromatin; oocyte extract; DNA photoproduct; DNA synthesis; DNA repair; Xenopus laevis; nucleosome; in vitro; ultraviolet radiation; eukaryotic DNA; oocyte

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1998
• Tom: 45
• Numer: 2
• Opis fizyczny: p.595-603,fig.

Twórcy

autor

Widlak P.

• Institute of Oncology, Wybrzeze Armii Krajowej 15, 44-100 Gliwice, Poland

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