Molecular screening of apple [Malus domestica] cultivars and breeding clones for their resistance to fire blight

• Autorzy: Keller-Przybylkowicz S , Lewandowski M. , Korbin M.

Warianty tytułu

PL

Ocena przydatnosci wybranych markerow molekularnych QTL do szybkiej oceny odmian i klonow hodowlanych jabloni [Malus domestica] jako donorow odpornosci na zaraze ogniowa w programach hodowli

• Języki publikacji: EN

Abstrakty

EN

Three SCAR and two SSR molecular markers located on Lg 3 and Lg7 were used for early selection of apple genotypes. The purpose of the selection was to find a potential donor for resistance to fire blight (FB) in an apple breeding programme. This study was carried out on 35 breeding clones, and registered cultivars originating from seven countries. They were all characterized as having different levels of sus­ceptibility to FB in field conditions. The number of generated markers varied from 1-2 to 4-5, depending on the genotype. For the majority of the tested genotypes, strong interactions were observed between data concerning plant behaviour in the field and the presence/number of DNA markers. For nine genotypes, however, correla­tions between phenotypic and molecular study were not found with selected QTLs.

PL

Trzy markery SCAR i 2 markery SSR z grup sprzężeń Lg 3 i 7 wykorzystano do wczesnej selekcji genotypów jabłoni, będących potencjalnymi donorami genów od­porno ści na zarazę ogniową. Badania prowadzono na 35 genotypach (klony hodowla­ne i zarejestrowane odmiany) z siedmiu krajów, różniących się stopniem podatności na zaraz ę ogniową. Liczba amplikonów generowanych na matrycy DNA pochodzą­cych z testowanych genotypów wynosiła od 1-2 do 4-5 fragmentów. Dla większości testowanych roślin obserwowano ścisłe interakcje między liczbą uzyskanych marke­rów a opisanym przez różnych autorów stopniem podatności tych genotypów na zara­zę ogniową w warunkach polowych. Dla 9 genotypów nie znaleziono korelacji mię­dzy danymi fenotypowymi a wynikami badań molekularnych, opartych na wybranych QTL

Słowa kluczowe

EN

molecular screening; apple; Malus domestica; apple cultivar; breeding clone; resistance; fire blight; SCAR molecular marker; SSR molecular marker; molecular marker; breeding programme; DNA marker

• Wydawca: -
• Czasopismo: Journal of Fruit and Ornamental Plant Research
• Rocznik: 2009
• Tom: 17
• Numer: 2
• Opis fizyczny: p.31-43,fig.,ref.

Twórcy

autor

Keller-Przybylkowicz S

• Research Institute of Pomology and Floriculture, Pomologiczna 18, 96-100 Skierniewice, Poland

autor

Lewandowski M.

autor

Korbin M.

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